US4749780A
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Biologically active fragments of human antihemophilic factor and method for preparation thereof
Assignee: Pfizer Health AB
•
Filing: 1986-03-04
•
Granted: 1988-06-07
•
Expires: 2006-03-04
Abstract
Abstract Novel, biologically active fragments of human antihemophilic factor, processes for their preparation, pharmaceutical preparations containing them and the use of such fragments in the treatment of patients suffering from hemophilia.
Inventors
- 👤 Lars-Olof Andersson
- 👤 Nanna Forsman
- 👤 Kerstin E. I. Larsen
- 👤 Annelie B. Lundin
- 👤 Bohdan Pavlu
- 👤 Inga H. Sandberg
- 👤 Karin M. Sewerin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 00:34
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61P; A61P7/00; A61P7/04; E; E02; E02D; E02D31/00; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S930/00; Y10S930/01; Y10S930/10
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
104 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weights 90,000 daltons and 80,000 daltons, respectively and having the aminoterminal amino acid sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Arg-Thr-Thr-, respectively and having the aminoacid composition: ______________________________________
90 kD 80 kD
______________________________________
Cys 1.4 1.3
Asp 6.8 4.7
Asn 4.2 4.5
Thr 5.3 6.1
Ser 7.8 8.3
Glu 6.1 5.7
Gln 3.2 5.7
Pro 5.4 4.5
Gly 5.8 5.7
Ala 5.0 4.8
Val 6.8 5.4
Met 2.6 3.5
Ile 4.7 5.7
Leu 9.6 7.7
Tyr 5.1 4.4
Phe 4.9 5.7
Trp 1.8 2.2
Lys 5.5 5.4
His 3.4 3.5
Arg 4.7 5.0
______________________________________
Claim 2
Dependent Claim
104 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weights 90,000 daltons and 80,000 daltons, respectively and having the aminoterminal amino acid sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Arg-Thr-Thr-, respectively and having the aminoacid composition: ______________________________________
90 kD 80 kD
______________________________________
Cys 1.4 1.3
Asp 6.8 4.7
Asn 4.2 4.5
Thr 5.3 6.1
Ser 7.8 8.3
Glu 6.1 5.7
Gln 3.2 5.7
Pro 5.4 4.5
Gly 5.8 5.7
Ala 5.0 4.8
Val 6.8 5.4
Met 2.6 3.5
Ile 4.7 5.7
Leu 9.6 7.7
Tyr 5.1 4.4
Phe 4.9 5.7
Trp 1.8 2.2
Lys 5.5 5.4
His 3.4 3.5
Arg 4.7 5.0
______________________________________
Claim 3
Dependent Claim
102 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weight 115,000 daltons and 80,000 daltons, respectively and having the aminoterminal sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Thr-Arg-Thr-Thr-, respectively and having the aminoacid composition: ______________________________________
80 kD 115 kD
______________________________________
Cys 1.3 1.1
Asp 4.7 6.7
Asn 4.5 4.5
Thr 6.1 6.2
Ser 8.3 9.4
Glu 5.7 5.8
Gln 5.7 3.5
Pro 4.5 6.1
Gly 5.7 5.6
Ala 4.8 4.8
Val 5.4 5.8
Met 3.5 2.6
Ile 5.7 4.4
Leu 7.7 9.9
Tyr 4.4 4.3
Phe 5.7 4.7
Trp 2.2 1.5
Lys 5.4 5.4
His 3.5 3.4
Arg 5.0 4.4
______________________________________
Claim 4
Dependent Claim
102 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weight 115,000 daltons and 80,000 daltons, respectively and having the aminoterminal sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Thr-Arg-Thr-Thr-, respectively and having the aminoacid composition: ______________________________________
80 kD 115 kD
______________________________________
Cys 1.3 1.1
Asp 4.7 6.7
Asn 4.5 4.5
Thr 6.1 6.2
Ser 8.3 9.4
Glu 5.7 5.8
Gln 5.7 3.5
Pro 4.5 6.1
Gly 5.7 5.6
Ala 4.8 4.8
Val 5.4 5.8
Met 3.5 2.6
Ile 5.7 4.4
Leu 7.7 9.9
Tyr 4.4 4.3
Phe 5.7 4.7
Trp 2.2 1.5
Lys 5.4 5.4
His 3.5 3.4
Arg 5.0 4.4
______________________________________
Claim 5
Dependent Claim
102 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weight 130,000 daltons and 80,000 daltons, respectively and having the aminoterminal aminoacid sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Arg-Thr-Thr-, respectively and the aminoacid composition: ______________________________________
80 kD 130 kD
______________________________________
Cys 1.3 1.0
Asp 4.7 6.5
Asn 4.5 5.0
Thr 6.1 6.3
Ser 8.3 10.0
Glu 5.7 5.9
Gln 5.7 3.3
Pro 4.5 5.8
Gly 5.7 5.6
Ala 4.8 4.6
Val 5.4 5.5
Met 3.5 2.6
Ile 5.7 4.4
Leu 7.7 10.3
Tyr 4.4 3.8
Phe 5.7 4.5
Trp 2.2 1.5
Lys 5.4 5.9
His 3.5 3.3
Arg 5.0 4.3
______________________________________
Claim 6
Dependent Claim
102 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weight 130,000 daltons and 80,000 daltons, respectively and having the aminoterminal aminoacid sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Arg-Thr-Thr-, respectively and the aminoacid composition: ______________________________________
80 kD 130 kD
______________________________________
Cys 1.3 1.0
Asp 4.7 6.5
Asn 4.5 5.0
Thr 6.1 6.3
Ser 8.3 10.0
Glu 5.7 5.9
Gln 5.7 3.3
Pro 4.5 5.8
Gly 5.7 5.6
Ala 4.8 4.6
Val 5.4 5.5
Met 3.5 2.6
Ile 5.7 4.4
Leu 7.7 10.3
Tyr 4.4 3.8
Phe 5.7 4.5
Trp 2.2 1.5
Lys 5.4 5.9
His 3.5 3.3
Arg 5.0 4.3
______________________________________
Claim 7
Dependent Claim
101 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weights 150,000 daltons and 80,000 daltons, respectively, and having the aminoterminal sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Arg-Thr-Thr-, respectively, and the aminoacid composition: ______________________________________
80 kD 150 kD
______________________________________
Cys 1.3 0.8
Asp 4.7 6.0
Asn 4.5 5.7
Thr 6.1 6.2
Ser 8.3 9.7
Glu 5.7 6.3
Gln 5.7 3.8
Pro 4.5 5.8
Gly 5.7 5.6
Ala 4.8 4.4
Val 5.4 5.7
Met 3.5 2.7
Ile 5.7 4.3
Leu 7.7 10.1
Tyr 4.4 3.3
Phe 5.7 4.4
Trp 2.2 1.4
Lys 5.4 6.6
His 3.5 3.3
Arg 5.0 4.0
______________________________________
Claim 8
Dependent Claim
101 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weights 150,000 daltons and 80,000 daltons, respectively, and having the aminoterminal sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Arg-Thr-Thr-, respectively, and the aminoacid composition: ______________________________________
80 kD 150 kD
______________________________________
Cys 1.3 0.8
Asp 4.7 6.0
Asn 4.5 5.7
Thr 6.1 6.2
Ser 8.3 9.7
Glu 5.7 6.3
Gln 5.7 3.8
Pro 4.5 5.8
Gly 5.7 5.6
Ala 4.8 4.4
Val 5.4 5.7
Met 3.5 2.7
Ile 5.7 4.3
Leu 7.7 10.1
Tyr 4.4 3.3
Phe 5.7 4.4
Trp 2.2 1.4
Lys 5.4 6.6
His 3.5 3.3
Arg 5.0 4.0
______________________________________
Claim 9
Dependent Claim
101 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weights 160,000 daltons and 80,000 daltons, respectively, and having the aminoterminal sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Thr-Arg-Thr-Thr-, respectively, and the aminoacid composition: ______________________________________
80 kD 160 kD
______________________________________
Cys 1.3 0.8
Asp 4.7 5.8
Asn 4.5 5.9
Thr 6.1 6.5
Ser 8.3 9.6
Glu 5.7 6.3
Gln 5.7 4.0
Pro 4.5 5.5
Gly 5.7 5.6
Ala 4.8 4.4
Val 5.4 5.5
Met 3.5 2.6
Ile 5.7 4.1
Leu 7.7 10.1
Tyr 4.4 3.3
Phe 5.7 4.5
Trp 2.2 1.3
Lys 5.4 6.8
His 3.5 3.2
Arg 5.0 4.2
______________________________________
Claim 10
Dependent Claim
101 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weights 160,000 daltons and 80,000 daltons, respectively, and having the aminoterminal sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Thr-Arg-Thr-Thr-, respectively, and the aminoacid composition: ______________________________________
80 kD 160 kD
______________________________________
Cys 1.3 0.8
Asp 4.7 5.8
Asn 4.5 5.9
Thr 6.1 6.5
Ser 8.3 9.6
Glu 5.7 6.3
Gln 5.7 4.0
Pro 4.5 5.5
Gly 5.7 5.6
Ala 4.8 4.4
Val 5.4 5.5
Met 3.5 2.6
Ile 5.7 4.1
Leu 7.7 10.1
Tyr 4.4 3.3
Phe 5.7 4.5
Trp 2.2 1.3
Lys 5.4 6.8
His 3.5 3.2
Arg 5.0 4.2
______________________________________
Claim 11
Dependent Claim
102 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weights 180,000 daltons and 80,000 daltons, respectively, and having the aminoterminal aminoacid sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Thr-Arg-Thr-Thr-, respectively, and the aminoacid composition: ______________________________________
80 kD 180 kD
______________________________________
Cys 1.3 0.8
Asp 4.7 5.7
Asn 4.5 5.8
Thr 6.1 6.6
Ser 8.3 10.0
Glu 5.7 6.2
Gln 5.7 4.2
Pro 4.5 5.7
Gly 5.7 5.3
Ala 4.8 4.5
Val 5.4 5.3
Met 3.5 2.4
Ile 5.7 4.4
Leu 7.7 10.0
Tyr 4.4 3.2
Phe 5.7 4.4
Trp 2.2 1.2
Lys 5.4 6.9
His 3.5 3.2
Arg 5.0 4.3
______________________________________
Claim 12
Dependent Claim
102 words
Active fragment of human Factor VIII characterized by containing two peptide chains having molecular weights 180,000 daltons and 80,000 daltons, respectively, and having the aminoterminal aminoacid sequences Ala-Thr-Arg-Arg-Tyr-Tyr- and Glu-Ile-Thr-Arg-Thr-Thr-, respectively, and the aminoacid composition: ______________________________________
80 kD 180 kD
______________________________________
Cys 1.3 0.8
Asp 4.7 5.7
Asn 4.5 5.8
Thr 6.1 6.6
Ser 8.3 10.0
Glu 5.7 6.2
Gln 5.7 4.2
Pro 4.5 5.7
Gly 5.7 5.3
Ala 4.8 4.5
Val 5.4 5.3
Met 3.5 2.4
Ile 5.7 4.4
Leu 7.7 10.0
Tyr 4.4 3.2
Phe 5.7 4.4
Trp 2.2 1.2
Lys 5.4 6.9
His 3.5 3.2
Arg 5.0 4.3
______________________________________
References Cited (Backward Citations)
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