Patent US4748113A successfully refreshed from Google Patents.
US4748113A
Expired / Lapsed
US
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Compositions and methods for the diagnosis of gastrointestinal disorders involving urease
Assignee: MEDQUEST Pty Ltd
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Filing: 1985-06-13
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Granted: 1988-05-31
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Expires: 2005-06-13
Abstract
Abstract Compositions for diagnosis of gastrointestinal disorders comprising urea, a bactericide, an indicator having a pK a of from about 6.5 to about 8.5, and water, wherein the composition has a pH of from about 5.0 to about 6.5 and wherein the pH is at least about one pH unit lower than the pK a of the indicator. Preferably the compositions contain a gelling agent, such as agar. Also preferably the composition contain a buffer. The invention also provides methods for diagnosis of gastrointestinal disorders in a human or lower animal subject, comprising the steps of obtaining a sample of gastric material from the subject, contacting the sample with a composition of this invention, and observing any color change, wherein a color change indicates the presence of a gastrointestinal disorder. Also provided are devices for use in diagnosis of gastrointestinal disorders.
Inventors
- 👤 Barry J. Marshall
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/58; C12Q1/02; C12Q1/04; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/205
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
120 words
A composition for the diagnosis of gastrointestinal disorders in a human or lower animal subject by detection of urease in gastric material of the subject, comprising: (a) urea, at a concentration of from about 10 to about 40 grams per liter; (b) a bactericide which substantially inhibits the growth of urease-producing organisms, at a concentration of from about 1 to about 5 grams per liter; (c) an indicator having a pK a of from about 6.5 to about 8.5, at an effective concentration; and (d) water; wherein said composition has a pH of from about 5.0 to about 6.5, and the pH of said composition is at least about one pH unit lower than the pK a of said indicator.
Claim 2
Dependent Claim
120 words
A composition for the diagnosis of gastrointestinal disorders in a human or lower animal subject by detection of urease in gastric material of the subject, comprising: (a) urea, at a concentration of from about 10 to about 40 grams per liter; (b) a bactericide which substantially inhibits the growth of urease-producing organisms, at a concentration of from about 1 to about 5 grams per liter; (c) an indicator having a pK a of from about 6.5 to about 8.5, at an effective concentration; and (d) water; wherein said composition has a pH of from about 5.0 to about 6.5, and the pH of said composition is at least about one pH unit lower than the pK a of said indicator.
Claim 3
Dependent Claim
24 words
A composition, according to claim 1, wherein said indicator is present at a concentration of from about 2 to about 100 milligrams per liter.
Claim 4
Dependent Claim
20 words
A composition, according to claim 2, wherein said indicator has a pK a of from about 7.0 to about 8.0.
Claim 5
Dependent Claim
12 words
A composition, according to claim 3, wherein said indicator is phenol red.
Claim 6
Dependent Claim
19 words
A composition, according to claim 3, wherein said composition has a pH of from about 5.0 to about 6.0.
Claim 7
Dependent Claim
10 words
A composition, according to claim 1, additionally comprising a buffer.
Claim 8
Dependent Claim
24 words
A composition, according to claim 1, additionally comprising a gelling agent, at a concentration of from about 5 to about 50 grams per liter.
Claim 9
Dependent Claim
14 words
A composition, according to claim 7, wherein said gelling agent is a non-nutritive agar.
Claim 10
Dependent Claim
24 words
A composition, according to claim 8, wherein said agar is present at a concentration of from about 10 to about 20 grams per liter.
Claim 11
Independent Claim
157 words
A method for detection of a gastrointestinal disorder in a human or lower animal subject by detection of urease in gastric material of the subject, comprising the steps of: (a) obtaining a sample of gastric material from said subject; (b) contacting said sample with a composition comprising: urea, at a concentration of about 10 to about 40 grams per liter; a bactericide which substantially inhibits the growth of urease-producing organisms, at a concentration of about 1 to about 5 grams per liter; an indicator having a pK a of about 6.5 to about 8.5, at an effective concentration; and water; said composition having a pH of about 5.0 to about 6.5, said pH being about one pH unit less than the pK a of said indicator; and (c) observing the color of said composition; wherein a change of color of said composition indicates the presence of urease and the existence of a gastrointestinal disorder in the subject.
Claim 12
Independent Claim
157 words
A method for detection of a gastrointestinal disorder in a human or lower animal subject by detection of urease in gastric material of the subject, comprising the steps of: (a) obtaining a sample of gastric material from said subject; (b) contacting said sample with a composition comprising: urea, at a concentration of about 10 to about 40 grams per liter; a bactericide which substantially inhibits the growth of urease-producing organisms, at a concentration of about 1 to about 5 grams per liter; an indicator having a pK a of about 6.5 to about 8.5, at an effective concentration; and water; said composition having a pH of about 5.0 to about 6.5, said pH being about one pH unit less than the pK a of said indicator; and (c) observing the color of said composition; wherein a change of color of said composition indicates the presence of urease and the existence of a gastrointestinal disorder in the subject.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-05-31 | 1991-11-30 | 1992-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-31 | 1995-11-30 | 1996-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-31 | 1999-11-30 | 2000-05-31 | $7700.00 | Window Closed / Lapsed |