US4748113A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Compositions and methods for the diagnosis of gastrointestinal disorders involving urease
Assignee: MEDQUEST Pty Ltd
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Filing: 1985-06-13
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Granted: 1988-05-31
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Expires: 2005-06-13
Abstract
Abstract Compositions for diagnosis of gastrointestinal disorders comprising urea, a bactericide, an indicator having a pK a of from about 6.5 to about 8.5, and water, wherein the composition has a pH of from about 5.0 to about 6.5 and wherein the pH is at least about one pH unit lower than the pK a of the indicator. Preferably the compositions contain a gelling agent, such as agar. Also preferably the composition contain a buffer. The invention also provides methods for diagnosis of gastrointestinal disorders in a human or lower animal subject, comprising the steps of obtaining a sample of gastric material from the subject, contacting the sample with a composition of this invention, and observing any color change, wherein a color change indicates the presence of a gastrointestinal disorder. Also provided are devices for use in diagnosis of gastrointestinal disorders.
Inventors
- 👤 Barry J. Marshall
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:22
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/58; C12Q1/02; C12Q1/04; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/205
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
120 words
A composition for the diagnosis of gastrointestinal disorders in a human or lower animal subject by detection of urease in gastric material of the subject, comprising: (a) urea, at a concentration of from about 10 to about 40 grams per liter; (b) a bactericide which substantially inhibits the growth of urease-producing organisms, at a concentration of from about 1 to about 5 grams per liter; (c) an indicator having a pK a of from about 6.5 to about 8.5, at an effective concentration; and (d) water; wherein said composition has a pH of from about 5.0 to about 6.5, and the pH of said composition is at least about one pH unit lower than the pK a of said indicator.
Claim 2
Dependent Claim
120 words
A composition for the diagnosis of gastrointestinal disorders in a human or lower animal subject by detection of urease in gastric material of the subject, comprising: (a) urea, at a concentration of from about 10 to about 40 grams per liter; (b) a bactericide which substantially inhibits the growth of urease-producing organisms, at a concentration of from about 1 to about 5 grams per liter; (c) an indicator having a pK a of from about 6.5 to about 8.5, at an effective concentration; and (d) water; wherein said composition has a pH of from about 5.0 to about 6.5, and the pH of said composition is at least about one pH unit lower than the pK a of said indicator.
Claim 3
Dependent Claim
24 words
A composition, according to claim 1, wherein said indicator is present at a concentration of from about 2 to about 100 milligrams per liter.
Claim 4
Dependent Claim
20 words
A composition, according to claim 2, wherein said indicator has a pK a of from about 7.0 to about 8.0.
Claim 5
Dependent Claim
12 words
A composition, according to claim 3, wherein said indicator is phenol red.
Claim 6
Dependent Claim
19 words
A composition, according to claim 3, wherein said composition has a pH of from about 5.0 to about 6.0.
Claim 7
Dependent Claim
10 words
A composition, according to claim 1, additionally comprising a buffer.
Claim 8
Dependent Claim
24 words
A composition, according to claim 1, additionally comprising a gelling agent, at a concentration of from about 5 to about 50 grams per liter.
Claim 9
Dependent Claim
14 words
A composition, according to claim 7, wherein said gelling agent is a non-nutritive agar.
Claim 10
Dependent Claim
24 words
A composition, according to claim 8, wherein said agar is present at a concentration of from about 10 to about 20 grams per liter.
Claim 11
Independent Claim
157 words
A method for detection of a gastrointestinal disorder in a human or lower animal subject by detection of urease in gastric material of the subject, comprising the steps of: (a) obtaining a sample of gastric material from said subject; (b) contacting said sample with a composition comprising: urea, at a concentration of about 10 to about 40 grams per liter; a bactericide which substantially inhibits the growth of urease-producing organisms, at a concentration of about 1 to about 5 grams per liter; an indicator having a pK a of about 6.5 to about 8.5, at an effective concentration; and water; said composition having a pH of about 5.0 to about 6.5, said pH being about one pH unit less than the pK a of said indicator; and (c) observing the color of said composition; wherein a change of color of said composition indicates the presence of urease and the existence of a gastrointestinal disorder in the subject.
Claim 12
Independent Claim
157 words
A method for detection of a gastrointestinal disorder in a human or lower animal subject by detection of urease in gastric material of the subject, comprising the steps of: (a) obtaining a sample of gastric material from said subject; (b) contacting said sample with a composition comprising: urea, at a concentration of about 10 to about 40 grams per liter; a bactericide which substantially inhibits the growth of urease-producing organisms, at a concentration of about 1 to about 5 grams per liter; an indicator having a pK a of about 6.5 to about 8.5, at an effective concentration; and water; said composition having a pH of about 5.0 to about 6.5, said pH being about one pH unit less than the pK a of said indicator; and (c) observing the color of said composition; wherein a change of color of said composition indicates the presence of urease and the existence of a gastrointestinal disorder in the subject.
References Cited (Backward Citations)
US3145086A
Cited
US3247051A
Cited
GB1112251A
Cited
FR5877M
Cited
US4153685A
Cited
US3873269A
Cited
GB1478742A
Cited
US4101382A
Cited
US4016268A
Cited
FR2442268A1
Cited
EP0018825A1
Cited
US4282316A
Cited
JPS5877663A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-31 | 1991-11-30 | 1992-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-31 | 1995-11-30 | 1996-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-31 | 1999-11-30 | 2000-05-31 | $7700.00 | Window Closed / Lapsed |