US4746551A
Expired / Lapsed
US
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Rehydratable polyacrylamide gels
Assignee: Proteome Sciences PLC
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Filing: 1984-08-24
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Granted: 1988-05-24
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Expires: 2004-08-24
Abstract
Abstract The present invention relates to rehydratable polyacrylamide gels. The rehydratable gels can be stored at room temperature for long periods of time and rehydrated with various materials to provide a suitable matrix for various electrophoretic separations.
Inventors
- 👤 Robert C. Allen
- 👤 Bertold J. Radola
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 08:27
CPC Classifications
C; C08; C08J; C08J3/00; C08K; C08K5/00; C08K5/0008; C08K5/005; C08K5/04; C08K5/05; C08K5/053; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; C08J2333/00; C08J2333/24; C08J2333/26
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
66 words
A process for the production of a rehydratable polyacrylamide gel comprising: (a) polymerizing acrylamide monomers on a hydrophilic support material to form a gel, wherein the resulting polyacrylamide is covalently bonded to the support; (b) removing from the gel any residual components of the polymerization; (c) adding a stabilizing amount of a stabilizer which consists essentially of one or more polysaccharides; and (d) drying the gel.
Claim 2
Dependent Claim
66 words
A process for the production of a rehydratable polyacrylamide gel comprising: (a) polymerizing acrylamide monomers on a hydrophilic support material to form a gel, wherein the resulting polyacrylamide is covalently bonded to the support; (b) removing from the gel any residual components of the polymerization; (c) adding a stabilizing amount of a stabilizer which consists essentially of one or more polysaccharides; and (d) drying the gel.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein said polysaccharide is added in the form of a solution.
Claim 4
Dependent Claim
20 words
The process of claim 1 wherein said polysaccharide is added in a rehydration step following partial drying of the gel.
Claim 5
Dependent Claim
10 words
The process of claim 1 wherein said polysaccharide is dextran.
Claim 6
Dependent Claim
27 words
The process of claim 1 wherein polysaccharide is added in amounts of from about 5% to about 30% by volume of the total volume of the mixture.
Claim 7
Dependent Claim
28 words
The process of claim 7 wherein said polysaccharide is added in amounts of from about 7% to about 10% by volume of the total volume of the mixture.
Claim 8
Dependent Claim
25 words
The process of claim 1 wherein said support material is a hydrophilic polyester sheet or glass plate that has been treated with a silane compound.
Claim 9
Dependent Claim
16 words
The process of claim 1 wherein said polyacrylamide gel is in the form of a sheet.
Claim 10
Dependent Claim
18 words
The process of claim 8 wherein said sheet is from about 30 microns to 500 microns in thickness.
Claim 11
Dependent Claim
19 words
The process of claim 9 wherein said sheet is from about 50 microns to about 300 microns in thickness.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-24 | 1991-11-23 | 1992-05-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-24 | 1995-11-23 | 1996-05-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-24 | 1999-11-23 | 2000-05-24 | $7700.00 | Window Closed / Lapsed |