Patent US4745069A successfully refreshed from Google Patents.
US4745069A
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Cloning vectors for expression of exogenous protein
Assignee: Eli Lilly and Co
•
Filing: 1984-03-06
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Granted: 1988-05-17
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Expires: 2004-03-06
Abstract
Abstract A recombinant DNA cloning vector useful for expressing exogenous protein is described, which comprises (a) a DNA segment containing a functional origin of replication; (b) one or more DNA segments, each of which conveys to a transformable host cell a property useful for selection when said vector is transformed into said host cell; and (c) a DNA segment comprising a sequence that defines, in tandem, (1) the promoter of a lipoprotein expression control sequence, (2) the 5' untranslated region of a lipoprotein expression control sequence and (3) a translation start codon followed, without interposition of a portion or all of a nucleotide sequence coding for endogenous protein, by a nucleotide sequence coding for an enterokinase cleavage site to which is joined, without interruption, a nucleotide sequence coding for an exogenous protein.
Inventors
- 👤 Nancy G. Mayne
- 👤 J. Paul Burnett
- 👤 Ramamoorthy Belegaje
- 👤 Hansen M. Hsiung
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:28
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/66; C12N15/70; C07; C07K; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/50; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
136 words
A recombinant DNA cloning vector useful for expressing exogenous protein, which comprises (a) a DNA segment containing a functional origin of replication; (b) one or more DNA segments, each of which conveys to a transformable host cell a property useful for selection when said vector is transformed into said host cell; and (c) a DNA segment comprising a sequence that defines in tandem, (1) the promoter of a lipoprotein gene (2) the 5' untranslated region of a lipoprotein gene and (3) a translation start codon followed, without interposition of a portion or all of a nucleotide sequence coding for endogenous protein, by a nucleotide sequence coding for an enterokinase cleavage site said site being a sequence of amino acid residues comprising Asp-Asp-Asp-Asp-Lys, to which is joined, without interruption, a nucleotide sequence coding for an exogenous protein.
Claim 2
Dependent Claim
136 words
A recombinant DNA cloning vector useful for expressing exogenous protein, which comprises (a) a DNA segment containing a functional origin of replication; (b) one or more DNA segments, each of which conveys to a transformable host cell a property useful for selection when said vector is transformed into said host cell; and (c) a DNA segment comprising a sequence that defines in tandem, (1) the promoter of a lipoprotein gene (2) the 5' untranslated region of a lipoprotein gene and (3) a translation start codon followed, without interposition of a portion or all of a nucleotide sequence coding for endogenous protein, by a nucleotide sequence coding for an enterokinase cleavage site said site being a sequence of amino acid residues comprising Asp-Asp-Asp-Asp-Lys, to which is joined, without interruption, a nucleotide sequence coding for an exogenous protein.
Claim 3
Dependent Claim
21 words
Cloning vector of claim 1, in which the exogenous protein nucleotide sequence codes for human growth hormone or bovine growth hormone.
Claim 4
Dependent Claim
27 words
Cloning vector of claim 1, in which the source of the nucleotide sequence of the promoter and that of the 5' untranslated region is a gram-negative bacterium.
Claim 5
Dependent Claim
28 words
Cloning vector of claim 3, in which the source of the nucleotide sequence of the promoter and that of the 5' untranslated region is the same gram-negative bacterium.
Claim 6
Dependent Claim
35 words
Cloning vector of claim 4, which contains in whole or in part the 3' untranslated region of a lipoprotein gene, said 3' untranslated region being located downstream of the sequence coding for said exogenous protein.
Claim 7
Dependent Claim
35 words
Cloning vector of claim 4, which contains in whole or in part the transcription termination region of a lipoprotein gene, said transcription termination region being located downstream of the sequence coding for said exogenous protein.
Claim 8
Dependent Claim
32 words
Cloning vector of claim 5, which contains in whole or in part the transcription termination region of a lipoprotein gene, said transcription termination region being located downstream of said 3' untranslated region.
Claim 9
Dependent Claim
26 words
Cloning vector of claim 4, in which the source of the nucleotide sequence of the promoter and that of the 5' untranslated region is E. coli.
Claim 10
Dependent Claim
18 words
Cloning vector of claim 8, in which the coding sequence for exogenous protein is for human growth hormone.
Claim 11
Dependent Claim
18 words
Cloning vector of claim 8, in which the coding sequence for exogenous protein is for bovine growth hormone.
Claim 12
Dependent Claim
26 words
Cloning vector of claim 7 in which the source of the nucleotide sequence of the promoter and that of the 5' untranslated region is E. coli.
Claim 13
Dependent Claim
18 words
Cloning vector of claim 11, in which the coding sequence for exogenous protein is for human growth hormone.
Claim 14
Dependent Claim
18 words
Cloning vector of claim 11, in which the coding sequence for exogenous protein is for bovine growth hormone.
Claim 15
Dependent Claim
27 words
Cloning vector of claim 2, in which the source of the nucleotide sequence of the promoter and that of the 5' untranslated region is a gram-negative bacterium.
Claim 16
Dependent Claim
28 words
Cloning vector of claim 14, in which the source of the nucleotide sequence of the promoter and that of the 5' untranslated region is the same gram-negative bacterium.
Claim 17
Dependent Claim
35 words
Cloning vector of claim 15, which contains in whole or in part the 3' untranslated region of a lipoprotein gene, said 3' untranslated region being located downstream of the sequence coding for said exogenous protein.
Claim 18
Dependent Claim
32 words
Cloning vector of claim 16, which contains in whole or in part the transcription termination region of a lipoprotein gene, said transcription termination region being located downstream of said 3' untranslated region.
Claim 19
Dependent Claim
26 words
Cloning vector of claim 17, in which the source of the nucleotide sequence of the promoter and that of the 5' untranslated region is E. coli.
Claim 20
Dependent Claim
18 words
Cloning vector of claim 1, in which the DNA sequence coding for the enterokinase cleavage site comprises ##STR3##
Claim 21
Dependent Claim
14 words
Cloning vector of claim 19, in which the enterokinase cleavage site codes for Phe-Pro-Leu-Asp-Asp-Asp-Asp-Lys.
Claim 22
Dependent Claim
18 words
Cloning vector of claim 20, in which the DNA sequence coding for the enterokinase cleavage site is ##STR4##
Claim 23
Dependent Claim
2 words
Plasmid pNM702.
Claim 24
Dependent Claim
2 words
Plasmid pNM702.
Claim 25
Dependent Claim
2 words
Plasmid pNM789.
Claim 26
Dependent Claim
2 words
Plasmid pNM789.
Claim 27
Dependent Claim
6 words
DNA sequence of the formula ##STR5##
Claim 28
Dependent Claim
6 words
DNA sequence of the formula ##STR5##
Claim 29
Dependent Claim
6 words
DNA sequence of the formula ##STR6##
Claim 30
Dependent Claim
6 words
DNA sequence of the formula ##STR6##
Claim 31
Dependent Claim
6 words
DNA sequence of the formula ##STR7##
Claim 32
Dependent Claim
6 words
DNA sequence of the formula ##STR7##
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-17 | 1991-11-16 | 1992-05-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-17 | 1995-11-16 | 1996-05-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-17 | 1999-11-16 | 2000-05-17 | $7700.00 | Window Closed / Lapsed |