US4739044A
Expired / Lapsed
US
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Method for derivitization of polynucleotides
Assignee: Amgen Inc
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Filing: 1985-06-13
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Granted: 1988-04-19
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Expires: 2005-06-13
Abstract
Abstract A method for the preparation of a 3' end functionalized polynucleotide is disclosed. An amine-functionalized solid phase support is treated sequentially with an anhydride, then with an ω-hydroxylamine. A polynucleotide is chemically synthesized on the treated support and is subsequently cleaved therefrom by hydrolysis of the amide bonds. A polynucleotide having a 3' free primary amine is recovered for use in hybridization assays and other uses.
Inventors
- 👤 Yitzhak Stabinsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 00:29
CPC Classifications
C; C07; C07H; C07H21/00
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
70 words
A method for preparing a functionalized polynucleotide comprising the steps of: forming an amide bond between a support-bound carboxyl group and an amino group of a hydroxylamine linker molecule; initiating synthesis of a polynucleotide on the hydroxyl group of the hydroxylamine linker molecule; hydrolyzing the amide bound between the amine and the support-bound carboxyl group; and recovering the polynucleotide connected to the amine group by way of the linker molecule.
Claim 2
Independent Claim
70 words
A method for preparing a functionalized polynucleotide comprising the steps of: forming an amide bond between a support-bound carboxyl group and an amino group of a hydroxylamine linker molecule; initiating synthesis of a polynucleotide on the hydroxyl group of the hydroxylamine linker molecule; hydrolyzing the amide bound between the amine and the support-bound carboxyl group; and recovering the polynucleotide connected to the amine group by way of the linker molecule.
Claim 3
Dependent Claim
15 words
The method according to claim 1 wherein said polynucleotide sequence is a deoxyribonucleic acid sequence.
Claim 4
Dependent Claim
15 words
The method according to claim 1 wherein said polynucleotide sequence is a ribonucleic acid sequence.
Claim 5
Dependent Claim
16 words
The method according to claim 1 wherein said hydroxylamine contains from 1 to 10 carbon atoms.
Claim 6
Dependent Claim
26 words
The method according to claim 4 wherein said hydroxylamine is selected from the group comprising methanolamine, ethanolamine, propanolamine, butanolamine, pentanolamine, hexanolamine, heptanolamine, octanolamine, nonanolamine and decanolamine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-19 | 1991-10-19 | 1992-04-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-19 | 1995-10-19 | 1996-04-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-19 | 1999-10-19 | 2000-04-19 | $7700.00 | Window Closed / Lapsed |