US4417046A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for isolating oligonucleotide product from a coupling reaction mixture
Assignee: Eli Lilly and Co
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Filing: 1981-08-24
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Granted: 1983-11-22
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Expires: 2001-08-24
Abstract
Abstract A polynucleotide product produced by coupling in the presence of a coupling agent (1) a nucleotide or oligonucleotide having a blocked 5'-hydroxyl group and a 3'-phosphate diester group and (2) a nucleotide or oligonucleotide having a blocked 3'-hydroxyl or a blocked 3'-phosphate diester group and a free 5'-hydroxyl group is recovered from the reaction mixture by addition of an organic solvent or mixture of organic solvents which precipitates the polynucleotide product from the reaction mixture while retaining the coupling agent in solution.
Inventors
- 👤 Hansen M. Hsiung
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 08:05
CPC Classifications
C; C07; C07H; C07H21/00
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
91 words
A process for recovering from a reaction mixture polynucleotide product produced by coupling in the presence of a coupling agent (1) a nucleotide or oligonucleotide having a blocked 5'-hydroxyl group and a 3'-phosphate diester group and (2) a nucleotide or oligonucleotide having a blocked 3'-hydroxyl or a blocked 3'-phosphate diester group and a free 5'-hydroxyl group, which comprises adding to the reaction mixture an organic solvent or a mixture of organic solvents comprising a substantially anhydrous ether thereby precipitating polynucleotide product from the reaction mixture while retaining coupling agent in solution.
Claim 2
Dependent Claim
91 words
A process for recovering from a reaction mixture polynucleotide product produced by coupling in the presence of a coupling agent (1) a nucleotide or oligonucleotide having a blocked 5'-hydroxyl group and a 3'-phosphate diester group and (2) a nucleotide or oligonucleotide having a blocked 3'-hydroxyl or a blocked 3'-phosphate diester group and a free 5'-hydroxyl group, which comprises adding to the reaction mixture an organic solvent or a mixture of organic solvents comprising a substantially anhydrous ether thereby precipitating polynucleotide product from the reaction mixture while retaining coupling agent in solution.
Claim 3
Dependent Claim
13 words
Process of claim 1, in which the substantially anhydrous ether is ethyl ether.
Claim 4
Dependent Claim
18 words
Process of claim 2, in which ethyl ether is used in combination with a low boiling aliphatic hydrocarbon.
Claim 5
Dependent Claim
15 words
Process of claim 3, in which the low boiling aliphatic hydrocarbon is pentane or hexane.
References Cited (Backward Citations)
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US3433782A
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US3534017A
Cited
US3709873A
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Cited
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Cited
US4210746A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-21 | 1987-05-23 | 1987-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-21 | 1991-05-23 | 1991-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-21 | 1995-05-23 | 1995-11-22 | $7700.00 | Window Closed / Lapsed |