US4738927A
Expired / Lapsed
US
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Gene coded for interleukin-2 polypeptide, recombinant DNA carrying the said gene, a living cell line possessing the recombinant DNA, and method for producing interleukin-2 using the said cell
Assignee: Ajinomoto Co Inc
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Filing: 1983-02-03
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Granted: 1988-04-19
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Expires: 2003-02-03
Abstract
Abstract A gene coded for a polypeptide which possesses interleukin-2 activity is isolated, and connected with a vector DNA which is capable of replicating in a procaryotic or eucaryotic cell at a position downstream of a promoter gene in the vector obtaining a recombinant DNA, with which the cell is transformed to produce interleukin-2.
Inventors
- 👤 Tadatsugu Taniguchi
- 👤 Masami Muramatsu
- 👤 Haruo Sugano
- 👤 Hiroshi Matsui
- 👤 Nobukazu Kashima
- 👤 Junji Hamuro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 20:46
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/55; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/79; C12N15/80; C12N15/81; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/141
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
49 words
A gene comprising a recombinant DNA molecule encoding a polypeptide possessing a biological activity of interleukin-2, wherein said biological activity is promotion of growth of a cytotoxic T-lymphocyte cell line, wherein said polypeptide has 132 to 134 amino acids in total in the amino acid sequence of the polypeptide.
Claim 2
Dependent Claim
49 words
A gene comprising a recombinant DNA molecule encoding a polypeptide possessing a biological activity of interleukin-2, wherein said biological activity is promotion of growth of a cytotoxic T-lymphocyte cell line, wherein said polypeptide has 132 to 134 amino acids in total in the amino acid sequence of the polypeptide.
Claim 3
Dependent Claim
17 words
The gene of claim 1, which encodes a polypeptide having Amino Acid Sequence II of FIG. 2(b).
Claim 4
Dependent Claim
17 words
The gene of claim 1, which encodes a polypeptide having Amino Acid Sequence III of FIG. 2(b).
Claim 5
Dependent Claim
18 words
The gene of claim 1, containing a base sequence corresponding to bases 108 to 506 in FIG. 2(a).
Claim 6
Dependent Claim
18 words
The gene of claim 1, containing a base sequence corresponding to bases 111 to 506 in FIG. 2(a).
Claim 7
Dependent Claim
79 words
A recombinant DNA which comprises a recombinant DNA encoding a polypeptide which possesses a biological activity of interleukin-2, wherein said activity is promotion of growth of a cytotoxic T-lymphocyte cell line, and a vector DNA capable of propagating in a procaryotic or eucaryotic cell, the coding sequence of said gene being located at a position downstream of a promoter sequence, wherein said polypeptide has 132 to 134 amino acids in total in the amino acid sequence of the polypeptide.
Claim 8
Dependent Claim
79 words
A recombinant DNA which comprises a recombinant DNA encoding a polypeptide which possesses a biological activity of interleukin-2, wherein said activity is promotion of growth of a cytotoxic T-lymphocyte cell line, and a vector DNA capable of propagating in a procaryotic or eucaryotic cell, the coding sequence of said gene being located at a position downstream of a promoter sequence, wherein said polypeptide has 132 to 134 amino acids in total in the amino acid sequence of the polypeptide.
Claim 9
Dependent Claim
13 words
The recombinant DNA of claim 6, wherein said procaryotic cell is Escherichia coli.
Claim 10
Dependent Claim
15 words
The recombinant DNA of claim 6, wherein said eucaryotic cell belongs to the genus Saccharomyces.
Claim 11
Dependent Claim
13 words
The recombinant DNA of claim 6, wherein said eucaryotic cell is Saccharomyces cereviceae.
Claim 12
Dependent Claim
22 words
The recombinant DNA of claim 6, wherein said eucaryotic cell is a monkey cell transformed with SV-40 constitutively expressing large T antigen.
Claim 13
Dependent Claim
23 words
The recombinant DNA of claim 6, wherein the base sequence of said recombinant DNA encodes the Amino Acid Sequence II of FIG. 2(b).
Claim 14
Dependent Claim
23 words
The recombinant DNA of claim 6, wherein the base sequence of said recombinant DNA encodes the Amino Acid Sequence III of FIG. 2(b).
Claim 15
Dependent Claim
23 words
The recombinant DNA of claim 6, wherein said recombinant DNA contains a base sequence corresponding to bases 108 to 506 in FIG. 2(a).
Claim 16
Dependent Claim
23 words
The recombinant DNA of claim 6, wherein said recombinant DNA contains the base sequence corresponding to bases 111 to 506 in FIG. 2(a).
Claim 17
Dependent Claim
86 words
A cell of a eucaryote or procaryote transformed with a recombinant DNA comprising a recombinant DNA encoding a polypeptide possessing a biological activity of interleukin-2 wherein said biological activity is promotion of growth of a cytotoxic T-lymphocyte cell line and wherein said polypeptide has 132 to 134 amino acids in total in the amino acid sequence of the polypeptide, and a vector DNA capable of propagating in said cell, and the coding sequence of said gene being located at a position downstream of a promoter sequence.
Claim 18
Dependent Claim
86 words
A cell of a eucaryote or procaryote transformed with a recombinant DNA comprising a recombinant DNA encoding a polypeptide possessing a biological activity of interleukin-2 wherein said biological activity is promotion of growth of a cytotoxic T-lymphocyte cell line and wherein said polypeptide has 132 to 134 amino acids in total in the amino acid sequence of the polypeptide, and a vector DNA capable of propagating in said cell, and the coding sequence of said gene being located at a position downstream of a promoter sequence.
Claim 19
Dependent Claim
14 words
The cell of claim 15, wherein said procaryotic cell belongs to the genus Escherichia.
Claim 20
Dependent Claim
12 words
The cell of claim 15, wherein said procaryotic cell is Escherichia coli.
Claim 21
Dependent Claim
14 words
The cell of claim 15, wherein said eucaryotic cell belongs to the genus Saccharomyces.
Claim 22
Dependent Claim
12 words
The cell of claim 15, wherein said eucaryotic cell is Saccharomyces cereviceae.
Claim 23
Dependent Claim
21 words
The cell of claim 15, wherein said eucaryotic cell is a monkey cell transformed with SV-40 constitutively expressing large T antigen.
Claim 24
Dependent Claim
22 words
The cell of claim 15, wherein the base sequence of said recombinant DNA encodes the Amino Acid Sequence II in FIG. 2(b).
Claim 25
Dependent Claim
22 words
The cell of calim 15, wherein the base sequence of said recombinant DNA encodes the Amino Acid Sequence III in FIG. 2(b).
Claim 26
Dependent Claim
22 words
The cell of calim 15, wherein the base sequence of said recombinant DNA encodes the Amino Acid Sequence III in FIG. 2(b).
Claim 27
Dependent Claim
22 words
The cell of claim 15, wherein said recombinant DNA contains a base sequence corresponding to bases 108 to 506 in FIG. 2(a).
Claim 28
Dependent Claim
22 words
The cell of claim 15, wherein said recombinant DNA contains a base sequence corresponding to bases 111 to 506 in FIG. 2(a).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-19 | 1991-10-19 | 1992-04-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-19 | 1995-10-19 | 1996-04-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-19 | 1999-10-19 | 2000-04-19 | $7700.00 | Window Closed / Lapsed |