Patent US5311523A successfully refreshed from Google Patents.
Patent US4737464A successfully refreshed from Google Patents.
US4737464A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Solid-state optical assay imaging apparatus
Assignee: Molecular Devices LLC
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Filing: 1985-09-26
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Granted: 1988-04-12
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Expires: 2005-09-26
Abstract
Abstract An apparatus for determining a characteristic of a signal modulating component related to an analyte of interest at an assay site by measuring light modulated by the component. A light source uniformly illuminates the assay site and a reference site. The light is directed from the sites onto a group of memory cells on the surface of a random access memory. The memory cells will decay from a logical 1 to a logical 0 due to the light over a period of time which is a function of the intensity of the light and the reference voltage of the memory cells marking the difference between a 1 and a 0. The digital state of each of the memory cells in the group is measured a predetermined exposure time. The measured states of a group of memory cells corresponding to the assay site are compared to the memory cell states for a reference group. The measured states are then analyzed to determine a characteristic of the analyte.
Inventors
- 👤 Harden M. McConnell
- 👤 Jonathan Briggs
- 👤 John W. Parce
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 11:10
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/251; G01N21/253; G01N21/27; G01N21/272; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N2201/00; G01N2201/04; G01N2201/0461; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10T; Y10T436/00; Y10T436/11
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
167 words
An apparatus for determining a characteristic of an analyte in a sample by measuring the light transmitted from an assay site in relation to the amount of signal modulating component present at said assay site, wherein the amount of said component is related to the presence of said analyte in said sample, said apparatus comprising: means for irradiating an assay site and a standard reference site with light while correcting for any nonuniformity in the light; a dynamic random access memory; means for directing light from said sites onto a first and a second group of memory cells, respectively, of said dynamic random access memory; means for exposing said memory cells to said directed light for a predetermined period of time; means for measuring the states of said memory cells after said exposure of said group of memory cells; means for comparing said measured states of said first group and said second group; and means for analyzing said compared states to determine said characteristic of said analyte.
Claim 2
Independent Claim
167 words
An apparatus for determining a characteristic of an analyte in a sample by measuring the light transmitted from an assay site in relation to the amount of signal modulating component present at said assay site, wherein the amount of said component is related to the presence of said analyte in said sample, said apparatus comprising: means for irradiating an assay site and a standard reference site with light while correcting for any nonuniformity in the light; a dynamic random access memory; means for directing light from said sites onto a first and a second group of memory cells, respectively, of said dynamic random access memory; means for exposing said memory cells to said directed light for a predetermined period of time; means for measuring the states of said memory cells after said exposure of said group of memory cells; means for comparing said measured states of said first group and said second group; and means for analyzing said compared states to determine said characteristic of said analyte.
Claim 3
Dependent Claim
27 words
The apparatus of claim 1 further comprising means for measuring the state of said memory cells at a plurality of different times to produce a gray scale.
Claim 4
Dependent Claim
29 words
The apparatus of claim 1 further comprising means for varying a reference voltage of said memory cells so that the sensitivity to light of said memory cells is varied.
Claim 5
Dependent Claim
38 words
The apparatus of claim 3 further comprising means for adjusting said reference voltage to a plurality of levels and means for measuring the states of said memory cells at each of said levels to produce a gray scale.
Claim 6
Dependent Claim
24 words
The apparatus of claim 1 further comprising means for focussing said light from said assay and reference sites onto said group of memory cells.
Claim 7
Dependent Claim
44 words
The apparatus of claim 1 further comprising means for irradiating a plurality of assay sites simultaneously and means for directing light from each said assay site to a different one of a plurality of groups of memory cells of said dynamic random access memory.
Claim 8
Dependent Claim
29 words
The apparatus of claim 1 further comprising means for changing the memory cells composing said group so that light from said assay site is substantially centered in said group.
Claim 9
Independent Claim
180 words
An apparatus for determining a characteristic of a plurality of analytes in a plurality of samples by measuring the light transmitted from a plurality of assay sites on a solid support in relation to the amount of signal modulating component present at each said assay site, wherein the amount of said component is related to the presence of analyte in a sample, said apparatus comprising: a light source for uniformly directing light to a plurality of assay sites and to a reference site; a dynamic random access memory having a plurality of memory cells, said memory having an exposed surface covered by a light-transmitting non-reflective plate; a lens for focussing light from each said site onto a separate block of said memory cells; a sequencer for sequentially reading said memory cells after a predetermined exposure time; means for comparing the states of said memory cells for each said block; means for comparing said measured states for a reference site block to each of the other blocks; and means for analyzing said compared states to determine said characteristic of said analytes.
Claim 10
Independent Claim
180 words
An apparatus for determining a characteristic of a plurality of analytes in a plurality of samples by measuring the light transmitted from a plurality of assay sites on a solid support in relation to the amount of signal modulating component present at each said assay site, wherein the amount of said component is related to the presence of analyte in a sample, said apparatus comprising: a light source for uniformly directing light to a plurality of assay sites and to a reference site; a dynamic random access memory having a plurality of memory cells, said memory having an exposed surface covered by a light-transmitting non-reflective plate; a lens for focussing light from each said site onto a separate block of said memory cells; a sequencer for sequentially reading said memory cells after a predetermined exposure time; means for comparing the states of said memory cells for each said block; means for comparing said measured states for a reference site block to each of the other blocks; and means for analyzing said compared states to determine said characteristic of said analytes.
Claim 11
Dependent Claim
24 words
The apparatus of claim 8 further comprising a computer for controlling the operation of said apparatus and analyzing the states of said memory cells.
Claim 12
Dependent Claim
61 words
The apparatus of claim 8 further comprising a plurality of lenses arranged to focus an image from a single site onto a plurality of blocks of memory cells on said dynamic random access memory and a plurality of filters, each filter being arranged to filter an image projected by one of said lenses onto one of said blocks of memory cells.
Claim 13
Dependent Claim
18 words
The apparatus of claim 8 further comprising means for applying reagents to said sites on said solid support.
Claim 14
Independent Claim
113 words
A method for determining a characteristic of an analyte in a sample, said method comprising the steps of: irradiating an assay site and a standard reference site with light while correcting for any non-uniformity in the light; directing light from said sites onto a first and a second group of memory cells, respectively, of a dynamic random access memory; exposing said memory cells to said directed light for a predetermined period of time; measuring the states of said memory cells after said exposure of said group of memory cells; comparing said measured states of said first group and said second group; and analyzing said compared states to determined said characteristic of said analyte.
Claim 15
Independent Claim
113 words
A method for determining a characteristic of an analyte in a sample, said method comprising the steps of: irradiating an assay site and a standard reference site with light while correcting for any non-uniformity in the light; directing light from said sites onto a first and a second group of memory cells, respectively, of a dynamic random access memory; exposing said memory cells to said directed light for a predetermined period of time; measuring the states of said memory cells after said exposure of said group of memory cells; comparing said measured states of said first group and said second group; and analyzing said compared states to determined said characteristic of said analyte.
Claim 16
Dependent Claim
29 words
The method of claim 12 wherein said step for measuring the state of said memory cells is performed at a plurality of different times to produce a gray scale.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-12 | 1991-10-12 | 1992-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-12 | 1995-10-12 | 1996-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-12 | 1999-10-12 | 2000-04-12 | $7700.00 | Window Closed / Lapsed |