US4725557A
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Production of fucosyl antigens and antibodies for recognizing same determination of cancer associated carbohydrate linkage using same and kit for the determination
Assignee: Otsuka Pharmaceutical Co Ltd
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Filing: 1983-05-28
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Granted: 1988-02-16
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Expires: 2003-05-28
Abstract
Abstract PCT No. PCT/JP83/00169 Sec. 371 Date Jan. 19, 1984 Sec. 102(e) Date Jan. 19, 1984 PCT Filed May 28, 1983 PCT Pub. No. WO83/04311 PCT Pub. Date Dec. 8, 1983.The invention relates to a process for producing a fucosyl antigen charcterized in that an oligosaccharide contaning an alpha -fucoyransoyl-(1->3)-, -(1->4)- or -(1->6)-galactoyransoyl group and serving as a hapten is reacted wtih a carrier protein to obtain a carbohydrate antigen and to a process for producing from the antigen an antibody having specific reactivity with cells of cancers of the digestive system, especially human colon carcinoma cells, and murine teratocarcinoma cells. The invention also relates to a method of determining a cancer associated crabohydrate linkage with use of such antibody capable of specifically recognizing specific carbohydrate linkage and to a cancer diagnosing kit containing the antibody.
Inventors
- 👤 Teruo Miyauchi
- 👤 Suguru Yonezawa
- 👤 Masayuki Ozawa
- 👤 Eiichi Sato
- 👤 Takashi Muramatsu
- 👤 Setsuzo Tejima
- 👤 Taku Chiba
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 15:30
CPC Classifications
C; C07; C07H; C07H21/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/575; G01N33/5756; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
23 words
A carbohydrate antigen comprising a complex of a carrier protein and an oligosacoharide selected from the group consisting of 3'-α-L-fucopyranosyl-α-lactose, 4'-α-L-fucopyranosyl-α-lactose and 6'-α-L-fucopyranosyl-α-lactose.
Claim 2
Dependent Claim
23 words
A carbohydrate antigen comprising a complex of a carrier protein and an oligosacoharide selected from the group consisting of 3'-α-L-fucopyranosyl-α-lactose, 4'-α-L-fucopyranosyl-α-lactose and 6'-α-L-fucopyranosyl-α-lactose.
Claim 3
Dependent Claim
14 words
The carbohydrate antigen as defined in claim 1 in which said oligosaccharide is 3'-α-L-fucopyranosyl-α-lactose.
Claim 4
Dependent Claim
17 words
The carbohydrate antigen as defined in claim 1 produced by binding the oligosaccharide with the carrier protein.
Claim 5
Dependent Claim
21 words
An antibody which is characterized by specifically recognizing an antigen selected from the group consisting of O-α-L-fucopyranoayl-(1→?3)-, -(1→4) and-(1→6) -O-α-D-galactopyranosyl group.
Claim 6
Dependent Claim
21 words
An antibody which is characterized by specifically recognizing an antigen selected from the group consisting of O-α-L-fucopyranoayl-(1→?3)-, -(1→4) and-(1→6) -O-α-D-galactopyranosyl group.
Claim 7
Dependent Claim
9 words
An antibody specific for the antigen of claim 1.
Claim 8
Dependent Claim
29 words
The antibody as defined in claim 4 in which said antibody is produced by administering to a mammal the carbohydrate antigen of claim 1 and collecting the resulting antibody.
Claim 9
Dependent Claim
12 words
The antibody as defined in claim 6 which specifically recognizes O-α-L-fucypyranosyl-(1→3)-O-β-D-galactopyranosyl group.
Claim 10
Dependent Claim
29 words
The antibody as defined in claim 4 which specifically recognizes a cancer associated carbohydrate linkage having a group selected from the group consisting of O-α-fucopyranosyl-(1→3)-, -(1→4)- and -(1→6)-O-β-D-galactopyranosyl group.
Claim 11
Dependent Claim
27 words
The antibody as defined in claim 8 in which said cancer associated carbohydrate linkage is produced by a human colon carcinoma cell or a murine teratocarcinoma cell.
Claim 12
Dependent Claim
32 words
The antibody as defined in claim 6 in which there is administered to a rabbit a carbohydrate antigen produced by reacting 3'-α-L-fucopyranosyl-α-lactose with bovine serum albumin according to an isothicyanate coupling method.
Claim 13
Dependent Claim
32 words
The antibody as defined in claim 6 in which there is administered to a rabbit a carbohydrate antigen produced by reacting 4'-α-L-fucopyranosyl-α-lactose with bovine serum albumin according to an isothiocyanate coupling method.
Claim 14
Dependent Claim
32 words
The antibody as defined in claim 6 in which there is administered to a rabbit a carbohydrate antigen produced by reacting 6'-α-L-fucopyranosyl-α-lactose with bovine serum albumin according to an isothiocyanate coupling method.
Claim 15
Dependent Claim
71 words
A method of determining a cancer associated carbohydrate linkage having a group selected from the group consisting of an O-β-fucopyranosyl-(1→3)-, -(1→4)- and -(1→6)O-β-D-galactopyranosyl group which comprises contacting the antibody of claim 4 with a boilogical sample and determining the presence or absence of the antibody combined with the sample as an indication of the presence of a cancer associated linkage selected from the group consisting of O-α-fucopyranosyl(1→3)-, -(1→4)- and -(1→6)-O-β-D-galactopyranosyl group.
Claim 16
Dependent Claim
17 words
The method as defined in claim 13 in which the antibody is the antibody of claim 6.
Claim 17
Dependent Claim
13 words
A cancer diagnosing kit comprising a container containing the antibody of claim 4.
Claim 18
Dependent Claim
13 words
A cancer diagnosing kit comprising a container containing the antibody of claim 5.
Claim 19
Dependent Claim
13 words
A cancer diagnosing kit comprising a container containing the antibody of claim 6.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-02-15 | 1991-08-17 | 1992-02-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-02-15 | 1995-08-17 | 1996-02-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-02-15 | 1999-08-17 | 2000-02-16 | $7700.00 | Window Closed / Lapsed |