US4078049A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Acetylcholine assay
Assignee: Hoffmann La Roche Inc
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Filing: 1977-02-28
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Granted: 1978-03-07
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Expires: 1997-02-28
Abstract
Abstract A sensitive immunoassay for acetylcholine is described. To prepare the acetylcholine selective antiserum, an antigen is made comprising 6-aminocaproic acid (2-trimethylaminoethyl) ester covalently bonded to an immunogenic carrier material through a peptide bond formed from said 6-amino group and carboxyl groups contained in said immunogenic carrier material and the antigen is injected into a suitable host animal to elicit the desired antiserum.
Inventors
- 👤 Arthur Martin Felix
- 👤 Sidney Spector
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 18:58
CPC Classifications
C; C07; C07C; C07C271/00; C07C271/06; C07C271/08; C07C271/10; C07C271/22; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/536; G01N33/537; G01N33/539; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/815; Y10S436/823
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
39 words
An antigen consisting essentially of a hapten, 6-aminocaproic acid (2-trimethylaminoethyl) ester and acid addition salts thereof, covalently bonded to an immunogenic carrier material through peptide bonds formed between said 6-amino group and carboxyl groups on said immunogenic carrier material.
Claim 2
Dependent Claim
39 words
An antigen consisting essentially of a hapten, 6-aminocaproic acid (2-trimethylaminoethyl) ester and acid addition salts thereof, covalently bonded to an immunogenic carrier material through peptide bonds formed between said 6-amino group and carboxyl groups on said immunogenic carrier material.
Claim 3
Dependent Claim
14 words
The antigen of claim 1 wherein said immunogenic carrier material is bovine serum albumin.
Claim 4
Dependent Claim
16 words
The antigen of claim 1 wherein said hapten is 6-aminocaproic acid (2-trimethylaminoethyl) ester p-toluenesulfonate hydrogen bromide.
Claim 5
Dependent Claim
59 words
An antibody specific to acetylcholine prepared by innoculating a host animal with an antigen consisting essentially of a hapten, 6-aminocaproic acid (2-trimethylaminoethyl) ester and acid addition salts thereof, covalently bonded to an immunogenic carrier material through peptide bonds formed between said 6-amino group and carboxyl groups on said immunogenic carrier material and collecting the serum from said host animal.
Claim 6
Dependent Claim
59 words
An antibody specific to acetylcholine prepared by innoculating a host animal with an antigen consisting essentially of a hapten, 6-aminocaproic acid (2-trimethylaminoethyl) ester and acid addition salts thereof, covalently bonded to an immunogenic carrier material through peptide bonds formed between said 6-amino group and carboxyl groups on said immunogenic carrier material and collecting the serum from said host animal.
Claim 7
Dependent Claim
24 words
The antibody of claim 4 wherein said antigen consists essentially of 6-aminocaproic acid (2-trimethylaminoethyl) ester p-toluenesulfonate hydrogen bromide covalently bonded to bovine serum albumin.
Claim 8
Independent Claim
94 words
A method for the assay of acetylcholine in a sample, which method comprises mixing said sample with a known amount of labelled acetylcholine and an antibody which will selectively complex with acetylcholine, measuring the degree of binding of said labelled acetylcholine compound with said antibody, and determining the amount of acetylcholine present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of acetylcholine with fixed amounts of said labelled acetylcholine and said antibody and determining the degree of binding for each known amount of acetylcholine.
Claim 9
Independent Claim
94 words
A method for the assay of acetylcholine in a sample, which method comprises mixing said sample with a known amount of labelled acetylcholine and an antibody which will selectively complex with acetylcholine, measuring the degree of binding of said labelled acetylcholine compound with said antibody, and determining the amount of acetylcholine present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of acetylcholine with fixed amounts of said labelled acetylcholine and said antibody and determining the degree of binding for each known amount of acetylcholine.
Claim 10
Dependent Claim
13 words
The method of claim 6 wherein said labelled acetylcholine is acetylcholine- 3 H.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-03-06 | 1981-09-05 | 1982-03-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-03-06 | 1985-09-05 | 1986-03-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-03-06 | 1989-09-05 | 1990-03-07 | $7700.00 | Window Closed / Lapsed |