Novel mutation of the c-K-ras oncogene activated in a human lung carcinoma

Claim 2

Purified DNA from a human c-K ras gene capable of transforming NIH3T3 mouse fibroblast cells to tumorigneic cells, said DNA containing a mutation in codon 61 of the second exon thereof.

Claim 3

Purified DNA of claim 1 wherein the c-K ras gene is derived from the human lung carcinoma line PR 310 (ATCC CRL- 8477).

Claim 4

Purified DNA of claim 1 wherein the mutation is a transversion in said codon 61.

Claim 5

Purified DNA of claim 3 wherein the transversion is in the third base of said codon 61.

Claim 6

Purified DNA of claim 4 wherein the transversion is an A→T transversion.

Claim 7

Purified DNA comprising the nucleotide sequence: __________________________________________________________________________ ATG ACT GAA TAT AAA CTT GTG GTA GTT GGA GCT GGT GGC GTA GGC AAG AGT GCC TTG ACG ATA CAG CTA ATT CAG AAT CAT TTT GTG GAC GAA TAT GAT CCA ACA ATA GAG GAT TCC TAC AGG AAG CAA GTA GTA ATT GAT GGA GAA ACC TGT CTC TTG GAT ATT CTC GAG ACA GCA GGT CAT GAG GAG TAC AGT GCA ATG AGG GAC CAG TAC ATG AGG ACT GGG GAG GGC TTT CTT TGT GTA TTT GCC ATA AAT AAT ACT AAA TCA TTT GAA GAT ATT CAC CAT TAT AGA GAA CAA ATT AAA AGA GTT AAG GAC TCT GAA GAT GTA CCT ATG GTC CTA GTA GGA AAT AAA TGT GAT TTG CCT TCT AGA ACA GTA GAC ACA AAA CAG GCT CAG GAC TTA GCA AGA AGT TAT GGA ATT CCT TTT ATT GAA ACA TCA GCA AAG ACA AGA __________________________________________________________________________ CAG

Claim 8

Purified DNA comprising the nucleotide sequence: __________________________________________________________________________ ATG ACT GAA TAT AAA CTT GTG GTA GTT GGA GCT GGT GGC GTA GGC AAG AGT GCC TTG ACG ATA CAG CTA ATT CAG AAT CAT TTT GTG GAC GAA TAT GAT CCA ACA ATA GAG GAT TCC TAC AGG AAG CAA GTA GTA ATT GAT GGA GAA ACC TGT CTC TTG GAT ATT CTC GAG ACA GCA GGT CAT GAG GAG TAC AGT GCA ATG AGG GAC CAG TAC ATG AGG ACT GGG GAG GGC TTT CTT TGT GTA TTT GCC ATA AAT AAT ACT AAA TCA TTT GAA GAT ATT CAC CAT TAT AGA GAA CAA ATT AAA AGA GTT AAG GAC TCT GAA GAT GTA CCT ATG GTC CTA GTA GGA AAT AAA TGT GAT TTG CCT TCT AGA ACA GTA GAC ACA AAA CAG GCT CAG GAC TTA GCA AGA AGT TAT GGA ATT CCT TTT ATT GAA ACA TCA GCA AAG ACA AGA __________________________________________________________________________ CAG

Claim 9

Purified DNA of claim 1 having the partial nucleotide sequence of the second coding exon.

Claim 10

Purified DNA of claim 2 having the partial nucleotide sequence of the second coding exon.

Claim 11

Purified DNA of claim 6 having the partial nucleotide sequence of the second coding exon.

Claim 12

A cloning vector containing the DNA of claim 1.

Claim 13

The cloning vector of claim 10 which is a bacterial plasmid or bacteriophage.

Claim 14

The cloning vector of claim 11 which is a pBR322 plasmid.

Claim 15

A cloning vector containing the DNA of claim 2.

Claim 16

The cloning vector of claim 13 which is a bacterial plasmid or bacteriophage.

Claim 17

The cloning vector of claim 14 which is a pBR322 plasmid.

Claim 18

A cloning vector containing the DNA of claim 6.

Claim 19

The cloning vector of claim 16 which is a bacterial plasmid or bacteriophage.

Claim 20

The cloning vector of claim 17 which is a pBR322 plasmid.