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US4722892A
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Monoclonal antibodies against metal chelates
Assignee: Hybritech Inc
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Filing: 1984-08-31
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Granted: 1988-02-02
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Expires: 2004-08-31
Abstract
Abstract Monoclonal Antibodies which are specific for a complex of a chelating agent and a metallic ion are described. The antibody has an association constant (K a ) for the complex which is at least about ten times greater than the K a for the chelating agent alone or its complex with another metal.
Inventors
- 👤 Claude F. Meares
- 👤 Gary S. David
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 18:42
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K51/10; A61K51/1093; C; C07; C07K; C07K16/00; C07K16/44; C07K16/46; C07K16/468; A61K2121/00; A61K38/00; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/813; Y10S530/00; Y10S530/808
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
56 words
A monoclonal antibody which exhibits specificity for a complex comprising a chelating agent and a first metallic ion, the antibody having an association constant (K A ) for the complex at least about ten times greater than the K A of the antibody for the chelating agent itself or its complex with any other metallic ion.
Claim 2
Dependent Claim
56 words
A monoclonal antibody which exhibits specificity for a complex comprising a chelating agent and a first metallic ion, the antibody having an association constant (K A ) for the complex at least about ten times greater than the K A of the antibody for the chelating agent itself or its complex with any other metallic ion.
Claim 3
Dependent Claim
14 words
A monoclonal antibody according to claim 1 wherein the metallic ion is a radionuclide.
Claim 4
Dependent Claim
42 words
A monoclonal antibody according to claims 1 or 2 wherein the monoclonal antibody is a bifunctional antibody, one specificity of which is against the complex of chelating agent and first metallic ion and the second against an antigen other than said complex.
Claim 5
Dependent Claim
17 words
A monoclonal antibody according to claim 3 wherein the second specificity is against a tumor associated antigen.
Claim 6
Dependent Claim
54 words
A monoclonal antibody according to claim 4 wherein the first metallic ion is a radionuclide whose product of radioactive decay is a metallic ion of a different atomic number and the K A of the antibody with the complex of decay product and chelating agent is less than about 1×10 7 M -1 .
Claim 7
Dependent Claim
34 words
A monoclonal antibody according to claim 5 wherein the K A of the antibody and the complex of the decay product and the chelating agent is less than about 1×10 6 M -1 .
Claim 8
Dependent Claim
74 words
A process for the treatment of a tumor having an antigen associated therewith comprising administering to a subject a monoclonal antibody according to claim 4 and allowing the antibody to localize at the situs of the tumor and then adminstering to the subject a composition comprising the chelate complex comprising the chelating agent and the first metallic ion, the metallic ion being a radionuclide that decays by emission of an electron or an alpha-particle.
Claim 9
Dependent Claim
12 words
A process according to claim 7 wherein the radionuclide is 90 Y.
Claim 10
Dependent Claim
53 words
A process according to claim 7 wherein the chelating agent is conjugated with a chemotherapeutic agent and the radionuclide decays to a product having a different atomic number and the K A of the antibody with the complex of decay product and chelating agent is less than about 1×10 7 M -1 .
Claim 11
Dependent Claim
30 words
A process according to claim 9 wherein the K A of the antibody with complex of decay product and chelating agent is less than about 1×10 6 M -1 .
Claim 12
Dependent Claim
92 words
A process for the detection of a metal ion in a fluid comprising: (a) contacting the fluid with a chelating agent bound to a solid phase under conditions which will form a chelate complex with the metal; (b) separating the fluid from the solid phase; (c) contacting the solid phase with a monoclonal antibody according to claim 1 which antibody has a label which permits its presence to be detected and/or quantified; (d) separating unbound antibody from antibody bound to the complex; (e) detecting the labeled antibody bound to the chelate complex.
Claim 13
Dependent Claim
27 words
A process according to claim 11 wherein the amount of bound or unbound labeled antibody is determined and related to the concentration of ion in the fluid.
Claim 14
Dependent Claim
20 words
A process according to claim 11 or 12 wherein the label is a radioactive element, fluorescent element or an enzyme.
References Cited (Backward Citations)
US4474893A
Cited
US4472509A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-02-01 | 1991-08-03 | 1992-02-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-02-01 | 1995-08-03 | 1996-02-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-02-01 | 1999-08-03 | 2000-02-02 | $7700.00 | Window Closed / Lapsed |