US4474893A
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Recombinant monoclonal antibodies
Assignee: The University of Texas MD Anderson Cancer Center
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Filing: 1981-07-01
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Granted: 1984-10-02
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Expires: 2001-07-01
Abstract
Abstract Antibodies having binding affinity for two desired antigens, hereinafter "recombinant monoclonal antibodies"; recombinant monoclonal antibodies produced by a quadroma cell or a trioma cell; and methods for producing recombinant monoclonal antibodies by means of a quadroma cell or a trioma cell, wherein a quadroma cell is the fusion product of a hybridoma cell which produces an antibody having specific binding affinity to one desired antigen and a hybridoma cell which produces an antibody having specific binding affinity for another desired antigen, and wherein a trioma cell is the fusion product of a hybridoma cell which produces an antibody having specific binding affinity to one desired antigen and a lymphocyte which produces an antibody having specific binding affinity to another desired antigen.
Inventors
- 👤 Christopher L. Reading
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:24
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/46; C07K16/468; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S436/00; Y10S436/804; Y10S436/819; Y10S530/00; Y10S530/806; Y10S530/808; Y10S530/809; Y10S530/863; Y10S530/864
Claims Analysis
Total Claims: 55
Claim 1
Independent Claim
10 words
An antibody with binding affinity for two different desired antigens.
Claim 2
Dependent Claim
10 words
An antibody with binding affinity for two different desired antigens.
Claim 3
Dependent Claim
15 words
The antibody of claim 1, wherein one of the two antigens is a tumor-specific antigen.
Claim 4
Dependent Claim
15 words
The antibody of claim 1, wherein one of the two antigens is a cell-specific antigen.
Claim 5
Dependent Claim
15 words
The antibody of claim 1, wherein one of the two antigens is a tissue-specific antigen.
Claim 6
Dependent Claim
20 words
The antibody of any of claims 1, 2, 3 or 4, wherein one of the two antigens is an enzyme.
Claim 7
Dependent Claim
20 words
The antibody of any of claims 1, 2, 3 or 4, wherein one of the two antigens is a hapten.
Claim 8
Dependent Claim
21 words
The antibody of claim 1 or 2, wherein one of the two antigens is the toxic subunit of Ricinus communis toxin.
Claim 9
Dependent Claim
17 words
The antibody of claim 1 or 2, wherein one of the two antigens is specific for trinitrophenol.
Claim 10
Dependent Claim
10 words
The antibody of claim 6, wherein the hapten is radioactively-labeled.
Claim 11
Dependent Claim
26 words
The antibody of claim 1, wherein one of the two antigens is the B subunit of human chorionic gonadotropin and the other antigen is a hapten.
Claim 12
Dependent Claim
26 words
The antibody of claim 1, wherein one of the two antigens is the B subunit of human chorionic gonadotropin and the other antigen is horseradish peroxidase.
Claim 13
Dependent Claim
17 words
The antibody of claim 1 or 2, wherein one of the two antigens is fluorescein or rhodamine.
Claim 14
Dependent Claim
19 words
The antibody of claim 1, wherein one of the two antigens is fluorescein and the other antigen is rhodamine.
Claim 15
Dependent Claim
61 words
An antibody produced by a quadroma cell, wherein said antibody has specific binding affinity for two desired antigens and wherein said quadroma cell comprises the fusion product of: (a) a hyridoma cell which produces an antibody having specific binding affinity to one desired antigen, and (b) a hybridoma cell which produces an antibody having specific binding affinity for another desired antigen.
Claim 16
Dependent Claim
61 words
An antibody produced by a quadroma cell, wherein said antibody has specific binding affinity for two desired antigens and wherein said quadroma cell comprises the fusion product of: (a) a hyridoma cell which produces an antibody having specific binding affinity to one desired antigen, and (b) a hybridoma cell which produces an antibody having specific binding affinity for another desired antigen.
Claim 17
Dependent Claim
60 words
An antibody produced by a trioma cell, wherein said antibody has specific binding affinity for two desired antigens and wherein said trioma cell comprises the fusion product of: (a) a hybridoma cell which produces an antibody having specific binding affinity to one desired antigen, and (b) a lymphocyte which produces an antibody having specific binding affinity to another desired antigen.
Claim 18
Dependent Claim
60 words
An antibody produced by a trioma cell, wherein said antibody has specific binding affinity for two desired antigens and wherein said trioma cell comprises the fusion product of: (a) a hybridoma cell which produces an antibody having specific binding affinity to one desired antigen, and (b) a lymphocyte which produces an antibody having specific binding affinity to another desired antigen.
Claim 19
Dependent Claim
15 words
The antibody of claim 14, wherein one of the desired antigens is a tumor-specific antigen.
Claim 20
Dependent Claim
15 words
The antibody of claim 14, wherein one of the desired antigens is a cell-specific antigen.
Claim 21
Dependent Claim
15 words
The antibody of claim 14, wherein one of the desired antigens is a tissue-specific antigen.
Claim 22
Dependent Claim
18 words
The antibody of claim 14, 16, 17 or 18, wherein one of the desired antigens is an enzyme.
Claim 23
Dependent Claim
18 words
The antibody of claim 14, 16, 17 or 18, wherein one of the desired antigens is a hapten.
Claim 24
Dependent Claim
21 words
The antibody of claim 14 or 16, wherein one of the desired antigens is a toxic subunit of Ricinus communis toxin.
Claim 25
Dependent Claim
17 words
The antibody of claim 14 or 16, wherein one of the desired antigens is specific for trinitriphenol.
Claim 26
Dependent Claim
11 words
The antibody of claim 20, wherein the hapten is radioactively labelled.
Claim 27
Dependent Claim
26 words
The antibody of claim 14, wherein one of the desired antigens is the B subunit of human chorionic gonadotropin and the other antigen is a hapten.
Claim 28
Dependent Claim
27 words
The antibody of claim 14, wherein one of the two desired antigens is the B subunit of human chorionic gonadotropin and the other antigen is horseradish peroxidase.
Claim 29
Dependent Claim
16 words
The antibody of claim 14, wherein one of the two desired antigens is flourescein or rhodamine.
Claim 30
Dependent Claim
22 words
The antibody of claim 14 and 16, wherein one of the two desired antigens is fluorescein and the other antigen is rhodamine.
Claim 31
Dependent Claim
15 words
The antibody of claim 15, wherein one of the desired antigens is a tumor-specific antigen.
Claim 32
Dependent Claim
15 words
The antibody of claim 15, wherein one of the desired antigens is a cell-specific antigen.
Claim 33
Dependent Claim
15 words
The antibody of claim 15, wherein one of the desired antigens is a tissue-specific antigen.
Claim 34
Dependent Claim
18 words
The antibody of claim 15, 28, 29 and 30, wherein one of the desired antigens is an enzyme.
Claim 35
Dependent Claim
18 words
The antibody of claim 15, 28, 29 or 30, wherein one of the desired antigens is a hapten.
Claim 36
Dependent Claim
20 words
The antibody of claim 15 or 28, wherein one of the antigens is a toxic subunit of Ricinus communis toxin.
Claim 37
Dependent Claim
17 words
The antibody of claim 15 or 28, wherein one of the desired antigens is specific for trinitrophenol.
Claim 38
Dependent Claim
11 words
The antibody of claim 32, wherein the hapten is radioactively labelled.
Claim 39
Dependent Claim
26 words
The antibody of claim 15, wherein one of the desired antigens is the B subunit of human chorionic gonadotropin and the other antigen is a hapten.
Claim 40
Dependent Claim
27 words
The antibody of claim 15, wherein one of the two desired antigens is the B subunit of human chorionic gonadotropin and the other antigen is horseradish peroxidase.
Claim 41
Dependent Claim
18 words
The antibody of claim 15 or 28, wherein one of the two desired antigens is flourescein or rhodamine.
Claim 42
Dependent Claim
20 words
The antibody of claim 15, wherein one of the two desired antigens is fluorescein and the other antigen is rhodamine.
Claim 43
Dependent Claim
12 words
The antibody of claim 1, wherein said antibody is an IgM molecule.
Claim 44
Dependent Claim
12 words
The antibody of claim 14, wherein said antibody is an IgM molecule.
Claim 45
Dependent Claim
12 words
The antibody of claim 15, wherein said antibody is an IgM molecule.
Claim 46
Dependent Claim
12 words
The antibody of claim 1, wherein said antibody is an IgG molecule.
Claim 47
Dependent Claim
12 words
The antibody of claim 14, wherein said antibody is an IgG molecule.
Claim 48
Dependent Claim
12 words
The antibody of claim 15, wherein said antibody is an IgG molecule.
Claim 49
Independent Claim
35 words
A method of producing a recombinant monoclonal antibody comprising incubating a quadroma cell in culture or in the peritoneal cavity of a mouse, and separating soluble protein from the culture supernatant or ascites fluid, respectively.
Claim 50
Independent Claim
35 words
A method of producing a recombinant monoclonal antibody comprising incubating a quadroma cell in culture or in the peritoneal cavity of a mouse, and separating soluble protein from the culture supernatant or ascites fluid, respectively.
Claim 51
Dependent Claim
52 words
The method of claim 46, further comprising the step of passing the soluble protein sequentially through two affinity chromatographic columns each bearing one of two antigens for which the recombinant monoclonal antibody has binding affinity, immobilized on the column and eluting recombinant monoclonal antibody from each column with a chaotropic ion solution.
Claim 52
Dependent Claim
32 words
The method of claim 46, wherein the quadroma cell is formed by fusing two hybridoma cell lines under conditions permitting selection of the fusion product in the presence of the parental hybridomas.
Claim 53
Independent Claim
35 words
A method of producing a recombinant monoclonal antibody comprising incubating a trioma cell in culture or in the peritoneal cavity of a mouse, and separating soluble protein from the culture supernatant or ascites fluid, respectively.
Claim 54
Independent Claim
35 words
A method of producing a recombinant monoclonal antibody comprising incubating a trioma cell in culture or in the peritoneal cavity of a mouse, and separating soluble protein from the culture supernatant or ascites fluid, respectively.
Claim 55
Dependent Claim
52 words
The method of claim 49, further comprising the step of passing the soluble protein sequentially through two affinity chromatographic columns each bearing one of two antigens for which the recombinant monoclonal antibody has binding affinity, immobilized on the column and eluting recombinant monoclonal antibody from each column with a chaotropic ion solution.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-02 | 1988-04-02 | 1988-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-02 | 1992-04-02 | 1992-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-02 | 1996-04-02 | 1996-10-02 | $7700.00 | Window Closed / Lapsed |