Patent US4716120A successfully refreshed from Google Patents.
US4716120A
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US
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Stable allergenic extracts and methods
Assignee: BIOWHITTRAKER Inc A CORP OF
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Filing: 1985-11-25
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Granted: 1987-12-29
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Expires: 2005-11-25
Abstract
Abstract A storage-stable, high potency allergenic extract is prepared by ultrafiltration, retaining fractions having molecular weights of from 1000 to 100,000, and treating the solution with gel polymer and/or carbon absorbents. The extract is dried to a moisture content of less than one weight percent water. The purified solution and aqueous reconstituted solutions of the dried extract provide a transparent, colorless solution which has greatly increased stability, remaining transparent and colorless for extended periods.
Inventors
- 👤 Yuh-Geng Tsay
- 👤 Myron A. Beigler
- 👤 Emanuel Calenoff
- 👤 Gerald L. Friesen
- 👤 James L. Nichols
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:38
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/35; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/809
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
70 words
A substantially dried, purified allergenic extract substantially free from extracted components with molecular weights outside the range of 1000 to 100,000 daltons, having an allergen composition substantially the same as the original extract, and which upon aqueous reconstitution yields a transparent, colorless solution which has an increase in absorbance in the range of 400 to 700 nm of less than 0.01 O.D. after storage for 18 days at 22° C.
Claim 2
Dependent Claim
70 words
A substantially dried, purified allergenic extract substantially free from extracted components with molecular weights outside the range of 1000 to 100,000 daltons, having an allergen composition substantially the same as the original extract, and which upon aqueous reconstitution yields a transparent, colorless solution which has an increase in absorbance in the range of 400 to 700 nm of less than 0.01 O.D. after storage for 18 days at 22° C.
Claim 3
Dependent Claim
14 words
An insoluble support to which a purified allergenic extract of claim 1 is adhered.
Claim 4
Dependent Claim
21 words
An insoluble support to which a conjugate of the purified allergenic extract of claim 1 and a water-soluble protein is adhered.
Claim 5
Dependent Claim
33 words
The purified stabilized allergenic extract of claim 1 wherein the extract is obtained from a member selected from the group consisting of pollens, epidermals, glandular elements, molds, smuts, insects, insect venoms and foods.
Claim 6
Dependent Claim
11 words
The purified allergenic extract of claim 4 obtained from a pollen.
Claim 7
Dependent Claim
17 words
The purified allergenic extract of claim 4 obtained from an epidermal or glandular element of an animal.
Claim 8
Dependent Claim
11 words
The purified allergenic extract of claim 4 obtained from a mold.
Claim 9
Dependent Claim
11 words
The purified allergenic extract of claim 4 obtained from a smut.
Claim 10
Dependent Claim
11 words
The purified allergenic extract of claim 4 obtained from an insect.
Claim 11
Dependent Claim
12 words
The purified allergenic extract of claim 4 obtained from an insect venom.
Claim 12
Dependent Claim
11 words
The purified allergenic extract of claim 4 obtained from a food.
Claim 13
Dependent Claim
140 words
A process for increasing stability of an allergenic extract comprising (a) passing an aqueous solution of an allergenic extract through 100,000 and 1000 dalton ultrafilters and retaining substantially all of the fraction having a molecular weight of from 1000 to 100,000 to yield an allergenic extract; (b) contacting the allergenic extract obtained in Step (a) with a quantity of a carbon absorbent and with a gel polymer absorbent which forms a cage structure in aqueous media which is sufficient to clarify the extract, to yield a retained fraction; and (c) substantially drying the retained fraction obtained in Step (b) to yield an allergenic product which upon aqueous reconstitution yields a transparent, colorless solution which has an increase in absorbance in the range of 400 to 700 nm of less than 0.01 O.D. after storage for 18 days at 22° C.
Claim 14
Dependent Claim
140 words
A process for increasing stability of an allergenic extract comprising (a) passing an aqueous solution of an allergenic extract through 100,000 and 1000 dalton ultrafilters and retaining substantially all of the fraction having a molecular weight of from 1000 to 100,000 to yield an allergenic extract; (b) contacting the allergenic extract obtained in Step (a) with a quantity of a carbon absorbent and with a gel polymer absorbent which forms a cage structure in aqueous media which is sufficient to clarify the extract, to yield a retained fraction; and (c) substantially drying the retained fraction obtained in Step (b) to yield an allergenic product which upon aqueous reconstitution yields a transparent, colorless solution which has an increase in absorbance in the range of 400 to 700 nm of less than 0.01 O.D. after storage for 18 days at 22° C.
Claim 15
Dependent Claim
12 words
A purified allergenic extract consisting essentially of the product of claim 12.
Claim 16
Dependent Claim
19 words
The process of claim 12 wherein the carbon absorbent has an average particle size of less than 200 mesh.
Claim 17
Dependent Claim
12 words
A purified allergenic extract consisting essentially of the product of claim 14.
Claim 18
Dependent Claim
34 words
The process of claim 12 wherein the allergenic extract is a fat-free, aqueous extract of a member selected from the group consisting of pollens, epidermals, glandular element, molds, smuts, insects, insect venoms and foods.
Claim 19
Dependent Claim
12 words
A purified allergenic extract consisting essentially of the product of claim 16.
Claim 20
Dependent Claim
23 words
The process of claim 12 wherein the swelled polymer is selected from the group consisting of a polysaccharide, polyacrylamide, polyvinylpyrrolidone and mixtures thereof.
Claim 21
Dependent Claim
12 words
A purified allergenic extract consisting essentially of the product of claim 18.
Claim 22
Dependent Claim
17 words
The process of claim 18 wherein the swelled polymer is a polysaccharide derived from dextran or agarose.
Claim 23
Dependent Claim
12 words
A purified allergenic extract consisting essentially of the product of claim 20.
Claim 24
Dependent Claim
29 words
The process of claim 20 wherein the carbon absorbent is activated charcoal having a particle size of less than 200 mesh and the swelled polymer is derived from dextran.
Claim 25
Dependent Claim
12 words
A purified allergenic extract consisting essentially of the product of claim 22.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-12-28 | 1991-06-29 | 1991-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-12-28 | 1995-06-29 | 1995-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-12-28 | 1999-06-29 | 1999-12-29 | $7700.00 | Window Closed / Lapsed |