US4716102A
Expired / Lapsed
US
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Purified AIDS-associated virus ARV-2
Assignee: University of California
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Filing: 1984-08-15
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Granted: 1987-12-29
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Expires: 2004-08-15
Abstract
Abstract A newly discovered family of AIDS-associated viruses, designated ARV, is described. The viruses were isolated from AIDS patients from San Francisco and (a) are type D retroviruses; (b) have Mg++-dependent reverse transcriptase activity; (c) induce human multinucleated cells without immortalizing the cells; (d) are replicable in HUT-78 human T cells; and (e) induce viral protein(s) in HUT-78 that binds to Ig from AIDS patients. The infected HUT-78 cells and immunogenic polypeptides derived from the viruses are useful for diagnosing AIDS.
Inventors
- 👤 Jay A. Levy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 22:06
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; C07; C07K; C07K16/00; C07K16/08; C07K16/10; C07K16/112; C07K16/114; C12N; C12N7/00; C12Q1/68; C12Q1/6813; C12Q1/6841; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56988; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16021; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/974; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/809; Y10S436/811; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
26 words
Biologically pure AIDS-associated retrovirus, ARV-2, said retrovirus having all the identifying characteristics of the ARV-2 retrovirus that has infected the human T-cell line, ATCC CRL 8597.
Claim 2
Dependent Claim
26 words
Biologically pure AIDS-associated retrovirus, ARV-2, said retrovirus having all the identifying characteristics of the ARV-2 retrovirus that has infected the human T-cell line, ATCC CRL 8597.
Claim 3
Dependent Claim
16 words
An immortal human T cell line chronically infected with an AIDS-associated retrovirus ARV-2, of claim 1.
Claim 4
Dependent Claim
19 words
The immortal human T cell line of claim 2 wherein the line is the human T cell line HUT-78.
Claim 5
Dependent Claim
158 words
A method of detecting antibodies to AIDS-associated retroviruses in a sample of an immunoglobulin-containing body fluid of a patient comprising: (a) incubating at a pH of about 7.2 to 7.4 the T cell line of claim 2 in a fixed form or a solid phase immunogenic ARV-2 polypeptide, derived from the retrovirus of claim 1, with the sample under conditions that promote antigen-antibody binding; (b) thereafter separating the cells or solid phase from the body fluid and washing the cells or solid phase to remove any residual unbound body fluid therefrom; (c) incubating the washed cells or solid phase with a labeled antibody to human Ig under conditions that promote antibody-antigen binding; (d) thereafter separating the cells or solid phase from the labeled antibody and washing the cells or solid phase to remove unbound labeled antibody therefrom; and (e) detecting immune complexes that include said AIDS-associated antibodies on or in the cells or solid phase via the label.
Claim 6
Dependent Claim
15 words
The method of claim 4 wherein the T cell line is HUT-78 infected with ARV-2.
Claim 7
Dependent Claim
22 words
The method of claim 4 wherein the label is a fluorochrome and the detecting is based on the fluorescence of the fluorochrome.
Claim 8
Dependent Claim
27 words
The method of claim 6 wherein the label is an enzyme and the detecting is done by reading the activity of the enzyme on a substrate spectrophotometrically.
Claim 9
Dependent Claim
44 words
A kit for detecting antibodies to ARV in a sample of an immunoglobulin-containing body fluid comprising: (a) fixed cells of the cell line of claim 2; (b) incubation medium for incubating the fixed cells with the body fluid; and (c) labeled anti-human Ig antibody.
Claim 10
Dependent Claim
44 words
A kit for detecting antibodies to ARV in a sample of an immunoglobulin-containing body fluid comprising: (a) fixed cells of the cell line of claim 3; (b) incubation medium for incubating the fixed cells with the body fluid; and (c) labeled anti-human Ig antibody.
References Cited (Backward Citations)
EP0138667B1
Cited
US4520113A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-12-28 | 1991-06-29 | 1991-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-12-28 | 1995-06-29 | 1995-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-12-28 | 1999-06-29 | 1999-12-29 | $7700.00 | Window Closed / Lapsed |