Patent US4710461A successfully refreshed from Google Patents.
US4710461A
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Promoter derived from chloroplast DNA
Assignee: Marubeni Corp
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Filing: 1984-08-20
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Granted: 1987-12-01
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Expires: 2004-08-20
Abstract
Abstract A promoter comprises a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 kbp, which is obtained by cleavage by restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene, said DNA fraction being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L. The promoter is integrated with a DNA having the same sequence as that of DNA derived from a procaryote into a vector.
Inventors
- 👤 Tohru Komano
- 👤 Kanji Ohyma
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 06:02
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/79; C12N15/82; C12N15/8216
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
97 words
A promoter comprising a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 kbp, which is obtained by cleavage by restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene, said DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L; said promoter being isolated from the rest of the chloroplast DNA of Marchantia polymorpha L.
Claim 2
Dependent Claim
97 words
A promoter comprising a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 kbp, which is obtained by cleavage by restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene, said DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L; said promoter being isolated from the rest of the chloroplast DNA of Marchantia polymorpha L.
Claim 3
Dependent Claim
126 words
A hybrid DNA comprising the following fractions (A) and (B): (A) a promoter comprising a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 kbp, which is obtained by cleavage by restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene, said DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L., and said promoter being isolated from the rest of the chloroplast DNA of Marchantia polymorpha L; and (B) A DNA having the same base sequence as that of DNA derived from a procaryote.
Claim 4
Dependent Claim
126 words
A hybrid DNA comprising the following fractions (A) and (B): (A) a promoter comprising a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 kbp, which is obtained by cleavage by restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene, said DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L., and said promoter being isolated from the rest of the chloroplast DNA of Marchantia polymorpha L; and (B) A DNA having the same base sequence as that of DNA derived from a procaryote.
Claim 5
Dependent Claim
129 words
A vector comprising a hybrid DNA comprising the following fractions (A) and (B): (A) a promoter comprising a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 Kbp, which is obtained by cleavage by a restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA. gene and a part of a 23SrRNA gene, said DNA fraction comprising all of a 16SrRNA. gene and a part of a 23SrRNA gene being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L., and said promoter being isolated from the rest of the chloroplast DNA of Marchantia polymorpha L; and (B) a DNA having the same sequence as that of DNA. derived from a protarugate.
Claim 6
Dependent Claim
129 words
A vector comprising a hybrid DNA comprising the following fractions (A) and (B): (A) a promoter comprising a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 Kbp, which is obtained by cleavage by a restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA. gene and a part of a 23SrRNA gene, said DNA fraction comprising all of a 16SrRNA. gene and a part of a 23SrRNA gene being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L., and said promoter being isolated from the rest of the chloroplast DNA of Marchantia polymorpha L; and (B) a DNA having the same sequence as that of DNA. derived from a protarugate.
Claim 7
Dependent Claim
28 words
A hybrid DNA as in claim 9 wherein the DNA derived from a procaryote is one which comprises at least a part of a plasmid of Escherichia coli.
Claim 8
Dependent Claim
15 words
A hybrid DNA as in claim 2 which is in the form of a plasmid.
Claim 9
Dependent Claim
27 words
A vector as in claim 3 wherein the DNA derived from a procaryote is one which comprises at least a part of a plasimid of Escherichia coli.
Claim 10
Dependent Claim
14 words
A vector as in claim 3 which is in the form of a plasmid.
Claim 11
Dependent Claim
39 words
A promoter as in claim 1, wherein said promoter has a higher promoter activity in E. coli, which is a procaryote, than that of said DNA fraction comprising all of a 16SrRNA gene and part of a 23SrRNA. gene.
Claim 12
Dependent Claim
40 words
A hybrid DNA as in claim 2, wherein said promoter has a higher promoter activity in E. coli, which is a procaryote, than that of said DNA fraction comprising all of a 16SrRNA gene and part of a 23SrRNA gene.
Claim 13
Dependent Claim
39 words
A vector as in claim 3, wherein said promoter has a higher promoter acitvity in E. coli, which is a procaryote, than that of said DNA fraction comprising all of a 16SrRNA gene and part of a 23SrRNA gene.
Claim 14
Independent Claim
161 words
A method for achieving expression of DNA having the same sequence as that of DNA derived from a eukaryote in a prokaryotic host cell, comprising transforming said host cell with a vector comprising (A) a promoter comprising a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 Kbp, which is obtained by cleavage by a restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA. gene and a part of a 23SrRNA. gene, said DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L. which is a eukaryote, said promoter being isolated from the rest of the chloroplast DNA of Marchantia polymorpha L. (B) a DNA having the same sequence as that of DNA derived from a procaryote, and permitting expression said eukaryotic promoter in said prokaryotic host.
Claim 15
Independent Claim
161 words
A method for achieving expression of DNA having the same sequence as that of DNA derived from a eukaryote in a prokaryotic host cell, comprising transforming said host cell with a vector comprising (A) a promoter comprising a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 Kbp, which is obtained by cleavage by a restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA. gene and a part of a 23SrRNA. gene, said DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L. which is a eukaryote, said promoter being isolated from the rest of the chloroplast DNA of Marchantia polymorpha L. (B) a DNA having the same sequence as that of DNA derived from a procaryote, and permitting expression said eukaryotic promoter in said prokaryotic host.
Claim 16
Dependent Claim
27 words
A method as in claim 11, wherein the DNA derived from a procaryote is one which comprises at least a part of a plasmid of Escherichia coli.
Claim 17
Dependent Claim
16 words
A method as in claim 11 wherein said vector is in the form of a plasmid.
References Cited (Backward Citations)
No citation records indexed.
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