Patent US4707448A successfully refreshed from Google Patents.
US4707448A
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Immortal line of human fetal glial cells
Assignee: United States Department of Health and Human Services
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Filing: 1984-10-04
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Granted: 1987-11-17
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Expires: 2004-10-04
Abstract
Abstract The present invention discloses permanent establishment of an immortal line of fetal glial cells. The fetal glial cell line is capable of mitotically proliferating and continually growing in vitro under suitable culture media and environmental conditions. A method of producing immortalized human fetal glial cell line is also disclosed. The method comprises transfecting primary human fetal glial cells with an origin-defective mutant of SV40 virus, passaging the resulting astroglial cells through suitable number of cycles and obtaining the desired cell line. The cell line of the present invention is capable of supporting multiplication of JC virus.
Inventors
- 👤 Eugene O. Major
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:51
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0618; C12N5/0622; C12N15/00; C12N2510/00; C12N2510/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
26 words
A continuous and permanently established line of human fetal glial cell being a eucaryotic host for the growth of human viruses and producing homogeneous astroglial cells.
Claim 2
Dependent Claim
26 words
A continuous and permanently established line of human fetal glial cell being a eucaryotic host for the growth of human viruses and producing homogeneous astroglial cells.
Claim 3
Dependent Claim
13 words
The cell line of claim 1 at least supporting multiplication of JC virus.
Claim 4
Dependent Claim
10 words
The cell line of claim 1 being ATCC CRL 8621.
Claim 5
Independent Claim
74 words
A method of producing immortalized human fetal glial cell line comprising transfecting primary human fetal glial cells with an origin-defective mutant of SV40 virus, passaging the resulting astroglial cells through sufficient number of cycles to obtain a mitotically proliferating and continually growing fetal glial cells and recovering a continuous and permanently established line of human fetal glial cell for producing homogeneous astroglial cells and acting as eucaryotic host for the growth of human viruses.
Claim 6
Independent Claim
74 words
A method of producing immortalized human fetal glial cell line comprising transfecting primary human fetal glial cells with an origin-defective mutant of SV40 virus, passaging the resulting astroglial cells through sufficient number of cycles to obtain a mitotically proliferating and continually growing fetal glial cells and recovering a continuous and permanently established line of human fetal glial cell for producing homogeneous astroglial cells and acting as eucaryotic host for the growth of human viruses.
Claim 7
Dependent Claim
17 words
The method of claim 4 wherein said fetal glial cells support multiplication at least of JC virus.
Claim 8
Dependent Claim
19 words
An immortal line of human fetal glial cell being a eucaryotic host produced by the method of claim 4.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-16 | 1991-05-18 | 1991-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-16 | 1995-05-18 | 1995-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-16 | 1999-05-18 | 1999-11-17 | $7700.00 | Window Closed / Lapsed |