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US4692883A Expired / Lapsed US ⏱️ 0.0 years remaining

Automatic digital wavelength calibration system for a spectrophotometer

Assignee: Applied Biosystems Inc
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Filing: 1985-02-21
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Granted: 1987-09-08
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Expires: 2005-02-21
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Abstract

Abstract This invention is directed to a digital wavelength calibration system for a spectrophotometer, which includes a photodiode array that forms an output detector for the spectrophotometer, a light source for generating at least one reference emission line of known peak wavelength, a computer controlled mechanism for causing the peak of said reference line to fall in close proximity to the center of a preselected pixel of said photodiode array, peak location computing apparatus for computing the exact position data of the peak relative to the pixel center in terms of the pixel spacing and the ordinal number of the preselected pixel and for retaining this position data for subsequent wavelength computation, wavelength identification computing apparatus for computing the numerical relationship data of the ordinate number of each pixel to the wavelength falling on that pixel and for retaining this relationship data for subsequent correlation with data generated by the pixel, and apparatus for computational processing and display of the data correlated with the associated wavelength value.

Inventors

  • 👤 Michael R. Nelson
  • 👤 Roy E. Boostrom
  • 👤 Jerry E. Cahill
  • 👤 Tzong Hwang

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-27 13:15
CPC Classifications
G; G01; G01J; G01J3/00; G01J3/28; G01J3/02; G01J3/10; G01J2003/2866; G01J3/2803

Claims Analysis

Total Claims: 9
Claim 1
Independent Claim 173 words
A digital wavelength calibration system for a spectrophotometer comprising, in combination: a photodiode array forming an output detector for said spectrophotometer and having an ordered number of spaced pixels, each pixel being identifiable by an ordinal number; a light source for generating at least one reference emission line of known peak wavelength; means for causing the peak of said reference line to fall in close proximity to the center of a preselected pixel of said photodiode array; peak location means for computing the exact position data of said peak relative to said pixel center in terms of pixel spacing and the ordinal number of said preselected pixel and for retaining said position data for subsequent wavelength computation; wavelength identification means for computing the numerical relationship data of the ordinate number of each pixel to the wavelength falling on that pixel and for retaining said relationship data for subsequent correlation with data generated by said pixel, and means for computational processing and display of said pixel generated data correlated with the associated wavelength value.
Claim 2
Dependent Claim 173 words
A digital wavelength calibration system for a spectrophotometer comprising, in combination: a photodiode array forming an output detector for said spectrophotometer and having an ordered number of spaced pixels, each pixel being identifiable by an ordinal number; a light source for generating at least one reference emission line of known peak wavelength; means for causing the peak of said reference line to fall in close proximity to the center of a preselected pixel of said photodiode array; peak location means for computing the exact position data of said peak relative to said pixel center in terms of pixel spacing and the ordinal number of said preselected pixel and for retaining said position data for subsequent wavelength computation; wavelength identification means for computing the numerical relationship data of the ordinate number of each pixel to the wavelength falling on that pixel and for retaining said relationship data for subsequent correlation with data generated by said pixel, and means for computational processing and display of said pixel generated data correlated with the associated wavelength value.
Claim 3
Dependent Claim 63 words
A digital wavelength calibrating system according to claim 1 wherein said means for causing the peak of said reference line to fall in close proximity to the center of a preselected pixel includes mechanical interchange means for placing one of a plurality of diffraction gratings into position for dispersing the optical beam through the spectrophotometer when so directed by a programmed calibration routine.
Claim 4
Dependent Claim 27 words
A digital wavelength calibration system claim 2 further comprising means for controlling in preselected sequence a multiplicity of interchangeable filters and shutters in cooperation programmed calibration routine.
Claim 5
Dependent Claim 27 words
A digital wavelength calibratopm system according to claim 1 wherein said calibration system is connected to a grating spectrophotometer having at least one diffraction grating for dispersion.
Claim 6
Dependent Claim 38 words
A digital wavelength calibration system according to claim 4 wherein said at least one diffraction grating defines a zero order and the zero order of said at least one diffraction grating is also used as a reference line.
Claim 7
Dependent Claim 31 words
A digital wavelength calibration system according to claim 1 wherein said peak location means computes said position data based on a quadratic fit to the intensity envelope of said reference line.
Claim 8
Dependent Claim 42 words
A digital wavelength calibration system according to claim 1 wherein said wavelength identification means computes said numerical relationship data based on a quadratic fit to pixel ordinal number vs wavelength relationship in the grating spectrum spread across the face of said array.
Claim 9
Dependent Claim 36 words
A digital wavelength calibration system according to claim 1 wherein said light source is a deuterium arc lamp generating a 656.1 line and said reference emission line is the 656.1 line of said deuterium arc lamp.

References Cited (Backward Citations)

US3646331A Cited
US3922091A Cited
US4249244A Cited
US4323309A Cited
US4488811A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1990-09-07 1991-03-09 1991-09-08 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1994-09-07 1995-03-09 1995-09-08 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1998-09-07 1999-03-09 1999-09-08 $7700.00 Window Closed / Lapsed

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