US4692433A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and composition for regulating serum calcium levels of mammals
Assignee: University of California
•
Filing: 1985-08-01
•
Granted: 1987-09-08
•
Expires: 2005-08-01
Abstract
Abstract Method for regulating serum calcium levels in mammals by orally administering a composition which includes a polypeptide hormone such as parathyroid hormone (PTH) or calcitonin. The polypeptide hormone is protected from destruction in the digestive tract by encapsulation in liposomes. The liposome preferably includes a cationic amphiphilic agent so that the surface of the liposome has a net positive charge. Agents for enhancing the action of the liposome encapsulated hormones are also disclosed.
Inventors
- 👤 Karl Y. Hostetler
- 👤 Leonard J. Deftos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 11:40
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/22; A61K38/23; A61K38/29; A61K9/00; A61K9/10; A61K9/127
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
53 words
A method for regulating blood calcium levels in a mammal which comprises: orally administering to said mammal a blood calcium-regulating effective amount of a composition consisting essentially of an aqueous solution of a polypeptide hormone selected from the group consisting of parathyroid hormone and calcitonin, said polypeptide hormone being encapsulated in a liposome.
Claim 2
Independent Claim
53 words
A method for regulating blood calcium levels in a mammal which comprises: orally administering to said mammal a blood calcium-regulating effective amount of a composition consisting essentially of an aqueous solution of a polypeptide hormone selected from the group consisting of parathyroid hormone and calcitonin, said polypeptide hormone being encapsulated in a liposome.
Claim 3
Dependent Claim
13 words
A method according to claim 1 wherein said polypeptide hormone is parathyroid hormone.
Claim 4
Dependent Claim
18 words
A method according to claim 2 wherein said polypeptide hormone is the hPTH 1-34 fragment of parathyroid hormone.
Claim 5
Dependent Claim
12 words
A method according to claim 1 wherein said polypeptide hormone is calcitonin.
Claim 6
Dependent Claim
37 words
A method according to claim 1 wherein said liposome contains from about 1 to 20 molar percentage of an alkylamine having the general formula C n H 2n+1 NH 2 , where n is 12 to 20.
Claim 7
Dependent Claim
11 words
A method according to claim 5 wherein said alkylamine is stearylamine.
Claim 8
Dependent Claim
34 words
A method according to claim 1 including the additional step of administering salicylate, salicylamide, acetylsalicylate or methylsalicylate to said mammal to improve the blood calcium-regulating action of said polypeptide hormone encapsulated in said liposome.
Claim 9
Dependent Claim
23 words
A method according to claim 1 wherein said liposome is made from phospholipids selected from the group consisting of phosphatidylcholine, dimyristoylphosphatidylcholine and dipalmitoylphosphatidylcholine.
Claim 10
Dependent Claim
69 words
A composition for use in oral administration in mammals to regulate blood calcium levels, said composition consisting essentially of a polypeptide hormone selected from the group consisting of parathyroid hormone and calcitonin, said polypeptide hormone being encapsulated in a liposome, said liposome containing from about 1 to 20 molar percentage of an alkylamine having the general formula C n H 2n+1 NH 2 ,where n is 12 to 20.
Claim 11
Dependent Claim
69 words
A composition for use in oral administration in mammals to regulate blood calcium levels, said composition consisting essentially of a polypeptide hormone selected from the group consisting of parathyroid hormone and calcitonin, said polypeptide hormone being encapsulated in a liposome, said liposome containing from about 1 to 20 molar percentage of an alkylamine having the general formula C n H 2n+1 NH 2 ,where n is 12 to 20.
Claim 12
Dependent Claim
13 words
A composition according to claim 9 wherein said polypeptide hormone is parathyroid hormone.
Claim 13
Dependent Claim
12 words
A composition according to claim 9 wherein said polypeptide hormone is calcitonin.
Claim 14
Dependent Claim
20 words
A composition according to claim 9 wherein said polypeptide hormone is the hPTH 1-34 amino acid fragment of parathyroid hormone.
Claim 15
Dependent Claim
11 words
A composition according to claim 9 wherein said alkylamine is stearylamine.
Claim 16
Dependent Claim
23 words
A composition according to claim 9 wherein said liposome is made from phospholipids selected from the group consisting of phosphatidylcholine, dimyristoylyphosphatidylcholine and dipalmitoylphosphatidylcholine.
Claim 17
Dependent Claim
30 words
A method according to claim 1 including the additional step of administering aprotinin to said mammal to improve the blood calcium-regulating action of said polypeptide hormone encapsulated in said liposome.
Claim 18
Dependent Claim
13 words
A method according to claim 4 wherein said polypeptide hormone is salmon calcitonin.
Claim 19
Dependent Claim
13 words
A composition according to claim 11 wherein said polypeptide hormone is salmon calcitonin.
Claim 20
Dependent Claim
82 words
A composition for oral administration in mammals to regulate blood calcium levels, said composition comprising: a polypeptide hormone selected from the group consisting of parathyroid hormone and calcitonin, said polypeptide hormone being encapsulated in a liposome, said liposome containing from about 1 to 20 molar percentage of an alkylamine having a general formula C n H 2n+1 NH 2 , where n is 12 to 20; and a pharmaceutically acceptable aqueous buffer solution have a pH of between about 4.0 and 8.0.
Claim 21
Dependent Claim
82 words
A composition for oral administration in mammals to regulate blood calcium levels, said composition comprising: a polypeptide hormone selected from the group consisting of parathyroid hormone and calcitonin, said polypeptide hormone being encapsulated in a liposome, said liposome containing from about 1 to 20 molar percentage of an alkylamine having a general formula C n H 2n+1 NH 2 , where n is 12 to 20; and a pharmaceutically acceptable aqueous buffer solution have a pH of between about 4.0 and 8.0.
Claim 22
Dependent Claim
20 words
A composition for oral administration according to claim 18 wherein cysteine is added to said buffer solution as a preservative.
Claim 23
Dependent Claim
39 words
A composition for oral administration according to claim 18 wherein aprotinin, salicylate, salicylamide, acetylsalicylate or methylsalicylate is added to said buffer solution in an amount sufficient to improve the calcium-regulating action of said polypeptide hormone encapsulated in said liposome.
Claim 24
Dependent Claim
15 words
A composition for oral administration according to claim 18 wherein said polypeptide hormone is calcitonin.
Claim 25
Dependent Claim
14 words
A composition for oral administration according to claim 18 wherein said alkylamine is stearylamine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-09-07 | 1991-03-09 | 1991-09-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-09-07 | 1995-03-09 | 1995-09-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-09-07 | 1999-03-09 | 1999-09-08 | $7700.00 | Window Closed / Lapsed |