US4686319A
Expired / Lapsed
US
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Synthesis of genetic females and their use in hybrid seed production
Assignee: Rutgers, The State University of New Jersey
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Filing: 1985-09-25
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Granted: 1987-08-11
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Expires: 2005-09-25
Abstract
Abstract A synthetic method is described for the development of genetic females in squash (Cucurbita pepo L.) through crosses of monoecious varieties or inbreds followed by selection. The genetic females and true-breeding female lines can be propagated sexually following chemical induction of male flowers. Such female lines can be used as seed parents in production of hybrids as well as germplasm for future breeding programs. The new synthetic method is applicable to other monoecious species of plants.
Inventors
- 👤 Oved Shifriss
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:04
CPC Classifications
A; A01; A01H; A01H1/00; A01H1/02; Y; Y10; Y10S; Y10S47/00; Y10S47/01
Claims Analysis
Total Claims: 69
Claim 1
Independent Claim
129 words
A process for synthesizing true-breeding female lines of squash comprising: (a) selecting one monocious variety or inbred as seed parent (P 1 ) and another monoecious variety or inbred as the pollen parent (P 2 ), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup; (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self pollination of the F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (d); (f) selecting an F 2 segregate which has very strong female expression or is female; and (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generation.
Claim 2
Dependent Claim
129 words
A process for synthesizing true-breeding female lines of squash comprising: (a) selecting one monocious variety or inbred as seed parent (P 1 ) and another monoecious variety or inbred as the pollen parent (P 2 ), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup; (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self pollination of the F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (d); (f) selecting an F 2 segregate which has very strong female expression or is female; and (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generation.
Claim 3
Dependent Claim
18 words
A process of claim 1 wherein the squash parents are selected from a single species, Cucurbita pepo L.
Claim 4
Dependent Claim
17 words
A process of claim 2 wherein one of squash parents is a breeding line known as NJ260.
Claim 5
Dependent Claim
27 words
A process of claim 2 wherein one parent is a variety known as Jersey Golden Acorn, and the other parent is a breeding line known as NJ260.
Claim 6
Dependent Claim
13 words
A process of claim 4 wherein the synthesized true-breeding female line is NJ1.
Claim 7
Dependent Claim
13 words
A process of claim 4 wherein the synthesized true-breeding female line is NJ33-G.
Claim 8
Dependent Claim
20 words
A process of claim 2 wherein the fruits of the synthesized true-breeding female line are green at early bud stage.
Claim 9
Dependent Claim
20 words
A process of claim 2 wherein the fruits of the synthesized true-breeding female line are yellow at early bud stage.
Claim 10
Dependent Claim
18 words
A process of claim 1 wherein the squash parents are selected from a single species, Cucurbita ficifolia Bouche.
Claim 11
Dependent Claim
18 words
A process of claim 1 wherein the squash parents are selected from a single species, Cucurbita maxima Duch.
Claim 12
Dependent Claim
18 words
A process of claim 1 wherein the squash parents are selected from a single species, Cucurbita mixta Pang.
Claim 13
Dependent Claim
18 words
A process of claim 1 wherein the squash parents are selected from a single species, Cucurbita moschata Poir.
Claim 14
Dependent Claim
12 words
A process of claim 1 wherein both squash parents are strongly female.
Claim 15
Dependent Claim
15 words
A process of claim 1 wherein the squash parents belong to different species of Cucurbita.
Claim 16
Dependent Claim
29 words
A process of claim 1 in which hybrid sterility in F 1 generation is overcome by backcrossing to either one of the parents or to another variety or inbred.
Claim 17
Dependent Claim
128 words
A process for synthesizing true-breeding female lines of the Curcurbitaceae family comprising: (a) selecting one monoecious variety or inbred as seed parent (P 1 ) and another monoecious variety or inbred as pollen parent (P 2 ), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup: (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self-pollinating of F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (d); (f) selecting an F 2 segregate which has very strong female expression or is female; and (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generations.
Claim 18
Dependent Claim
128 words
A process for synthesizing true-breeding female lines of the Curcurbitaceae family comprising: (a) selecting one monoecious variety or inbred as seed parent (P 1 ) and another monoecious variety or inbred as pollen parent (P 2 ), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup: (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self-pollinating of F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (d); (f) selecting an F 2 segregate which has very strong female expression or is female; and (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generations.
Claim 19
Dependent Claim
128 words
A process for synthesizing true-breeding female lines in monoecious species of crop plants comprising: (a) selecting one monoecious variety or inbred as seed parent (P 1 ) and another monoecious variety or inbred as pollen parent (P2), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup. (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self-pollinating of F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (b); (f) selecting an F 2 segregate which has very strong female expression or is female; and (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generations.
Claim 20
Dependent Claim
128 words
A process for synthesizing true-breeding female lines in monoecious species of crop plants comprising: (a) selecting one monoecious variety or inbred as seed parent (P 1 ) and another monoecious variety or inbred as pollen parent (P2), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup. (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self-pollinating of F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (b); (f) selecting an F 2 segregate which has very strong female expression or is female; and (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generations.
Claim 21
Independent Claim
32 words
A method of producing hybrid squash seed using a true-breeding female line as seed parent in pollinating proximity to another line of squash which serves as pollen parent and harvesting the seed.
Claim 22
Independent Claim
32 words
A method of producing hybrid squash seed using a true-breeding female line as seed parent in pollinating proximity to another line of squash which serves as pollen parent and harvesting the seed.
Claim 23
Dependent Claim
17 words
A method of claim 18 wherein the species of the true-breeding female line is Cucurbita pepo L.
Claim 24
Dependent Claim
29 words
A method of claim 18 wherein the true-breeding female line is a descendant of a cross in which one of the parents was a breeding line known as NJ260.
Claim 25
Dependent Claim
39 words
A method of claim 18 wherein the true-breeding female line is a descendant of a cross in which one parent was a variety known as Jersey Golden Acorn and the other parent was a breeding line known as NJ260.
Claim 26
Dependent Claim
12 words
A method of claim 18 wherein the true-breeding female line is NJ1.
Claim 27
Dependent Claim
12 words
A method of claim 18 wherein the true-breeding female line is NJ33-G.
Claim 28
Dependent Claim
26 words
Hybrid squash seed produced by using a true-breeding female line as seed parent in pollinating proximity to another line of squash which served as pollen parent.
Claim 29
Dependent Claim
26 words
Hybrid squash seed produced by using a true-breeding female line as seed parent in pollinating proximity to another line of squash which served as pollen parent.
Claim 30
Dependent Claim
15 words
Hybrid squash seed of claim 24 wherein the true-breeding female line is Cucurbita pepo L.
Claim 31
Dependent Claim
29 words
Hybrid squash seed of claim 24 wherein the true-breeding line is a descendant of a cross in which one of the parents was a breeding line known as NJ260.
Claim 32
Dependent Claim
42 words
Hybrid squash seed of claim 24 wherein the true-breeding female line is a descendant of a cross in which one of the parents was the variety known as Jersey Golden Acorn and the other parent was a breeding line known as NJ260.
Claim 33
Dependent Claim
13 words
Hybrid squash seed of claim 24 wherein the true-breeding female line is NJ1.
Claim 34
Dependent Claim
12 words
Hybrid squash seed of claim 24 wherein the true-breeding line is NJ33-G.
Claim 35
Dependent Claim
36 words
A first generation squash plant, the plant having been grown from the seed resulting from crossing a true-breeding female line as seed parent in pollinating proximity to another line of squash which serves as pollen parent.
Claim 36
Dependent Claim
36 words
A first generation squash plant, the plant having been grown from the seed resulting from crossing a true-breeding female line as seed parent in pollinating proximity to another line of squash which serves as pollen parent.
Claim 37
Dependent Claim
20 words
A first generation squash plant of claim 30 wherein the parents are selected from a single species, Cucurbita pepo L.
Claim 38
Dependent Claim
32 words
A first generation squash plant of claim 30 wherein the true-breeding female line is a descendant of a cross in which one of the parents was a breeding line known as NJ260.
Claim 39
Dependent Claim
44 words
A first generation squash plant of claim 30 wherein the true-breeding female line is a descendant of a cross in which one of the parents was the variety known as Jersey Golden Acorn and the other parent was a breeding line known as NJ260.
Claim 40
Dependent Claim
16 words
A first generation squash plant of claim 30 in which the true-breeding female line is NJ1.
Claim 41
Dependent Claim
16 words
A first generation squash plant of claim 30 in which the true-breeding female line is NJ33-G.
Claim 42
Dependent Claim
10 words
Squash seed which when grown yields a true-breeding female line.
Claim 43
Dependent Claim
10 words
Squash seed which when grown yields a true-breeding female line.
Claim 44
Dependent Claim
19 words
Squash seed of claim 36 which when grown yields a true-breeding female line of the species Cucurbita peco L.
Claim 45
Dependent Claim
26 words
Squash seed of claim 36 which when grown yields a true-breeding female line which is a descendant of a cross in which NJ260 was a parent.
Claim 46
Dependent Claim
45 words
Squash seed of claim 36 which when grown yields a true-breeding female line which is a descendant of a cross in which one of the parents was the variety known as Jersey Golden Acorn and the other parent was a breeding line known as NJ260.
Claim 47
Dependent Claim
14 words
Squash seed of claim 36 which when grown yields the true-breeding female line NJ1.
Claim 48
Dependent Claim
14 words
Squash seed of claim 36 which when grown yields the true-breeding female line NJ33-G.
Claim 49
Dependent Claim
32 words
A true-breeding female line of squash selected from the following species of the botannical genus Cucurbita: C. ficifolia Bouche, C. maxima Duch., C. mixta Pang., C. moschata Poir, and C. peno L.
Claim 50
Dependent Claim
32 words
A true-breeding female line of squash selected from the following species of the botannical genus Cucurbita: C. ficifolia Bouche, C. maxima Duch., C. mixta Pang., C. moschata Poir, and C. peno L.
Claim 51
Dependent Claim
16 words
A true-breeding female line of squash of claim 42 wherein said line is Cucurbita ceoo L.
Claim 52
Dependent Claim
24 words
A true-breeding female line of squash of claim 42 wherein said line is a descendant of a cross in which NJ260 was a parent.
Claim 53
Dependent Claim
43 words
A true-breeding female line of squash of claim 42 wherein said line is a descendant of a cross in which one of the parents was the variety known as Jersey Golden Acorn and the other parent was a breeding line known as NJ260.
Claim 54
Dependent Claim
12 words
A true-breeding female line of squash of claim 42 which is NJ1.
Claim 55
Dependent Claim
12 words
A true-breeding female line of squash of claim 42 which is NJ33-G.
Claim 56
Dependent Claim
122 words
A process for synthesizing a true-breeding female line of squash comprising: (a) selecting an existing true-breeding female line as seed parent (P 1 ) and another line as pollen parent (P 2 ); (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self-pollination of the F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (d); (f) selecting an F 2 segregate which has intense female expression or is female and which has one or more improved horticultural characteristics; and (g) developing a true-breeding female line having the one or more improved horticultural characteristics, from said segregate through selection and self-pollination in subsequent filial generations.
Claim 57
Dependent Claim
122 words
A process for synthesizing a true-breeding female line of squash comprising: (a) selecting an existing true-breeding female line as seed parent (P 1 ) and another line as pollen parent (P 2 ); (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self-pollination of the F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (d); (f) selecting an F 2 segregate which has intense female expression or is female and which has one or more improved horticultural characteristics; and (g) developing a true-breeding female line having the one or more improved horticultural characteristics, from said segregate through selection and self-pollination in subsequent filial generations.
Claim 58
Dependent Claim
18 words
A process of claim 48 wherein the squash parents are selected from a single species, Cucurbita pepo L.
Claim 59
Dependent Claim
25 words
A process of claim 48 wherein the existing true-breeding female line is a descendant of a cross in which one of the parents was NJ260.
Claim 60
Dependent Claim
42 words
A process of claim 48 wherein the existing true-breeding female line is a descendant of a cross in which one of the parents was the variety known as Jersey Golden Acorn and the other parent was a breeding line known as NJ260.
Claim 61
Dependent Claim
13 words
A process of claim 48 wherein the existing true-breeding female line is NJ1.
Claim 62
Dependent Claim
13 words
A process of claim 48 wherein the existing true-breeding female line is NJ33-G.
Claim 63
Dependent Claim
112 words
A process for synthesizing new monoecious varieties of squash comprising: (a) selecting an existing true-breeding female line as seed parent (P 1 ) and a monoecious variety as pollen parent (P 2 ); (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self-pollination of the F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (d); (f) selecting an F 2 monoecious segregate having one or more improved horticultural characteristics; and (g) developing a monoecious variety having the one or more improved horticultural characteristics from said segregate through selection and self-pollination in subsequent filial generations.
Claim 64
Dependent Claim
112 words
A process for synthesizing new monoecious varieties of squash comprising: (a) selecting an existing true-breeding female line as seed parent (P 1 ) and a monoecious variety as pollen parent (P 2 ); (b) crossing P 1 and P 2 ; (c) growing F 1 generation from the seed resulting from step (b); (d) self-pollination of the F 1 generation; (e) growing F 2 generation plants from the seeds resulting from step (d); (f) selecting an F 2 monoecious segregate having one or more improved horticultural characteristics; and (g) developing a monoecious variety having the one or more improved horticultural characteristics from said segregate through selection and self-pollination in subsequent filial generations.
Claim 65
Dependent Claim
18 words
A process of claim 54 wherein the squash parents are selected from a single species, Cucurbita pepo L.
Claim 66
Dependent Claim
25 words
A process of claim 54 wherein the existing true-breeding female line is a descendant of a cross in which one of the parents was NJ260.
Claim 67
Dependent Claim
42 words
A process of claim 54 wherein the existing true-breeding female line is a descendant of a cross in which one of the parents was the variety known as Jersey Golden Acorn and the other parent was a breeding line known as NJ260.
Claim 68
Dependent Claim
13 words
A process of claim 54 wherein the existing true-breeding female line is NJ1.
Claim 69
Dependent Claim
13 words
A process of claim 54 wherein the existing true-breeding female line is NJ33-G.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-08-10 | 1991-02-09 | 1991-08-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-08-10 | 1995-02-09 | 1995-08-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-08-10 | 1999-02-09 | 1999-08-11 | $7700.00 | Window Closed / Lapsed |