US4683295A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the preparation of anti-receptor antibodies
Assignee: Scripps Research Institute
•
Filing: 1984-05-24
•
Granted: 1987-07-28
•
Expires: 2004-05-24
Abstract
Abstract A method is discussed for the production of anti-receptor antibodies and to the detection and isolation of naturally occurring anti-receptor antibodies in animals and humans. The anti-receptor antibodies can be isolated from polyvalent anti-receptor antisera by immunodepletion of anti-immunoglobulin antibodies or by a single affinity purification step using antibody raised to an antigen recognized by the receptor.
Inventors
- 👤 Dennis A. Carson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 05:51
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; A61K39/39533; A61K39/39558; Y; Y10; Y10S; Y10S530/00; Y10S530/863; Y10S530/866
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
146 words
A method of preparing an anti-receptor internal image antibody comprising: (a) administering to a first animal host a receptor-containing composition in an amount sufficient to induce the production of an anti-receptor antiserum containing anti-receptor antibodies; (b) recovering said anti-receptor antibodies from the anti-receptor antiserum of the first animal host in partially purified form; (c) adminstering to a second animal host of the same species as the first animal host a ligand in an amount sufficient to induce the production of anti-ligand antiserum containig anti-ligand antibody, said ligand binding to said receptor; (d) recovering the anti-ligand antibody from the anti-ligand antiserum of the second animal host; (e) combining the anti-receptor antibody and the anti-ligand antibody to form a first admixture containing a first complex; (f) separating the first complex from the first admixture; (g) decomplexing the first complex; and (h) isolating substantially pure internal image anti-receptor antibody.
Claim 2
Independent Claim
146 words
A method of preparing an anti-receptor internal image antibody comprising: (a) administering to a first animal host a receptor-containing composition in an amount sufficient to induce the production of an anti-receptor antiserum containing anti-receptor antibodies; (b) recovering said anti-receptor antibodies from the anti-receptor antiserum of the first animal host in partially purified form; (c) adminstering to a second animal host of the same species as the first animal host a ligand in an amount sufficient to induce the production of anti-ligand antiserum containig anti-ligand antibody, said ligand binding to said receptor; (d) recovering the anti-ligand antibody from the anti-ligand antiserum of the second animal host; (e) combining the anti-receptor antibody and the anti-ligand antibody to form a first admixture containing a first complex; (f) separating the first complex from the first admixture; (g) decomplexing the first complex; and (h) isolating substantially pure internal image anti-receptor antibody.
Claim 3
Dependent Claim
57 words
The method according to claim 1 further including the steps of: (i) combining the substantially pure internal image anti-receptor antibody with the receptor-containing composition of step (a) to form a second admixture containing a second complex; (j) separating the second complex from the second admixture; and (k) decomplexing the second complex and isolating a substantially pure receptor.
Claim 4
Dependent Claim
49 words
The method according to claim 2 further including the step of: (1) administering to a third animal host the substantially pure receptor to produce an antiserum in the third animal host having a greater concentration of anti-receptor antibody than the concentration of anti-receptor antibody produced according to step (a).
Claim 5
Dependent Claim
18 words
The method according to claim 3 wherein the third animal host has been hyperimmunized to the receptor-containing composition.
Claim 6
Dependent Claim
14 words
The method according to claim 1 wherein the receptor-containing composition comprises whole animal cells.
Claim 7
Dependent Claim
18 words
The method according to claim 1 wherein the receptor-containing composition comprises plasma membranes prepared from whole animal cells.
Claim 8
Dependent Claim
15 words
The method according to claim 1 wherein the receptor-containing composition comprises a heterogeneous cell preparation.
Claim 9
Dependent Claim
27 words
The method according to claim 1 wherein the receptor can immunoreact with a ligand selected from the group consisting of a virus, parasite, toxin, drug or hormone.
Claim 10
Independent Claim
188 words
A method of preparing an anti-receptor internal image antibody comprising: (a) administering to a first animal host a receptor-containing composition in an amount sufficient to induce the production of an anti-receptor antiserum containing anti-receptor antibodies; (b) administering to a second animal host a ligand that binds with the receptor in an amount sufficient to induce the production of anti-ligand antiserum containing anti-ligand antibody; (c) recovering said anti-ligand antibody from the anti-ligand antiserum of the second animal host; (d) forming a coating on the surface of a solid phase support as an assay means, said coating comprising a predetermined amount of said anti-ligand antibody, to form a coated assay means; (e) contacting said coated assay means with said anti-receptor antiserum containing said anti-receptor antibody; (f) maintaining said contact for a predetermined period of time sufficient for the coating of said coated assay means to react with said anti-receptor antibody and deplete the anti-receptor antibody contained in the antiserum from the antiserum; (g) separating the depleted anti-receptor antiserum from the coated assay means; and (h) recovering the anti-receptor internal image antibody from the coated assay means in substantially pure form.
Claim 11
Independent Claim
188 words
A method of preparing an anti-receptor internal image antibody comprising: (a) administering to a first animal host a receptor-containing composition in an amount sufficient to induce the production of an anti-receptor antiserum containing anti-receptor antibodies; (b) administering to a second animal host a ligand that binds with the receptor in an amount sufficient to induce the production of anti-ligand antiserum containing anti-ligand antibody; (c) recovering said anti-ligand antibody from the anti-ligand antiserum of the second animal host; (d) forming a coating on the surface of a solid phase support as an assay means, said coating comprising a predetermined amount of said anti-ligand antibody, to form a coated assay means; (e) contacting said coated assay means with said anti-receptor antiserum containing said anti-receptor antibody; (f) maintaining said contact for a predetermined period of time sufficient for the coating of said coated assay means to react with said anti-receptor antibody and deplete the anti-receptor antibody contained in the antiserum from the antiserum; (g) separating the depleted anti-receptor antiserum from the coated assay means; and (h) recovering the anti-receptor internal image antibody from the coated assay means in substantially pure form.
Claim 12
Dependent Claim
14 words
The method according to claim 9 wherein the receptor-containing composition comprises whole animal cells.
Claim 13
Dependent Claim
18 words
The method according to claim 9 wherein the receptor-containing composition comprises plasma membranes prepared from whole animal cells.
Claim 14
Dependent Claim
15 words
The method according to claim 9 wherein the receptor-containing composition comprises a heterogeneous cell perparation.
Claim 15
Dependent Claim
27 words
The method according to claim 9 wherein the receptor can immunoreact with an ligand selected from the group consisting of a virus, parasite, toxin, drug or hormone.
Claim 16
Dependent Claim
24 words
The method in accordance with claim 9 wherein said assay means includes a microtiter tray having a plurality of wells whose surfaces are coated.
Claim 17
Dependent Claim
20 words
The method in accordance with claim 9 wherein said assay means includes a plurality of plastic beads having coated surfaces.
Claim 18
Dependent Claim
37 words
The method according to claim 1 wherein the receptor of said receptor-containing composition of step (a) is human rheumatoid factor and the ligand of said ligand-containing composition of step (c) is a human IgG Fc antibody fragment.
Claim 19
Dependent Claim
33 words
The method according to claim 9 wherein the receptor of said receptor-containing composition of step (a) is human rheumatoid factor and the ligand of step (b) is a human IgG Fc antibody fragment.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-07-27 | 1991-01-26 | 1991-07-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-07-27 | 1995-01-26 | 1995-07-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-07-27 | 1999-01-26 | 1999-07-28 | $7700.00 | Window Closed / Lapsed |