US4545986A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Timothy grass antigen specific anti-idiotypic antibodies
Assignee: RESEARCH Corp A NEW YORK NOT-FOR-PROFIT CORP
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Filing: 1982-06-22
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Granted: 1985-10-08
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Expires: 2002-06-22
Abstract
Abstract This invention provides a number of immunological reagents useful for the suppression of allergic reactions induced by Timothy-grass pollen allergen (or cross-reacting allergens thereof) and for methods of obtaining said reagents. The reagents include: (1) an anti-idiotypic antibody (anti-IgEid) directed to the allergen (Timothy grass antigen) binding site of an IgE immunoglobulin; (2) an anti-idiotypic antibody directed to the allergenic T-cell helper factor (THF) derived from Timothy grass pollen antigen stimulated lymphocytes; (3) unique methods for generating antigen-specific T-suppressor cells (TS); (4) a unique method for producing antigen-specific suppressor T cell factors (TSF); (5) a method for fractionation of TSF; and (6) a specific T cell factor, TSF2. The invention further provides pharmaceutical compositions comprising the above anti-idiotypic antibodies or suppressor factor derived from Ts cells.
Inventors
- 👤 Arthur Malley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:29
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/42; C07K16/4208; A; A61; A61K; A61K40/00; A61K40/10; A61K40/11; A61K40/13; A61K40/20; A61K40/22; A61K40/24; A61K40/40; A61K40/48; A61K40/482; C07K16/18; C07K16/24; A61K2239/00; A61K2239/31; A61K2239/38; A61K38/00; C07K2317/00; C07K2317/70; C07K2317/74; Y; Y10; Y10S; Y10S424/00; Y10S424/805; Y10S424/81; Y10S530/00; Y10S530/806; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/838; Y10S530/868
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
109 words
A method of preparing an anti-idiotypic antibody directed against (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific allergic antibody, IgE, said anti-idiotypic antibody, anti-IgE id , being specifically reactive with the idiotypic determinants on said IgE and (1) antigen B- and (2) cross reactive grass antigen-specific T and B cells comprising: (1) immunizing a first animal species with a source of antigen B, (2) collecting sera from said immunized first animal species, (3) separating IgE from said sera, (4) immunizing a second animal species with said IgE, (5) collecting sera from said immunized second animal species, and (6) separating said anti-IgE id antibody from said sera.
Claim 2
Independent Claim
109 words
A method of preparing an anti-idiotypic antibody directed against (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific allergic antibody, IgE, said anti-idiotypic antibody, anti-IgE id , being specifically reactive with the idiotypic determinants on said IgE and (1) antigen B- and (2) cross reactive grass antigen-specific T and B cells comprising: (1) immunizing a first animal species with a source of antigen B, (2) collecting sera from said immunized first animal species, (3) separating IgE from said sera, (4) immunizing a second animal species with said IgE, (5) collecting sera from said immunized second animal species, and (6) separating said anti-IgE id antibody from said sera.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein said second animal species is different from said first animal species.
Claim 4
Dependent Claim
19 words
The method of claim 1 wherein said first animal species is mouse and said second animal species is rabbit.
Claim 5
Dependent Claim
28 words
The method of claim 1 wherein said step (1) includes the step of hyperimmunizing said first animal species with a source of antigen B prior to the collection
Claim 6
Dependent Claim
5 words
The antibody anti-IgE id .
Claim 7
Dependent Claim
5 words
The antibody anti-IgE id .
Claim 8
Independent Claim
139 words
A method of preparing an anti-idiotypic antibody directed against (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific allergenic T-helper factor, T HF , said anti-idiotypic antibody, anti-T HF , being specifically reactive with the idiotypic determinants on said T HF and (1) antigen B- and (2) cross reactive grass antigen-specific T and B cells comprising: (1) immunizing a first animal species with photooxidized antigen B, (2) collecting T H - cells from said immunized first animal species, (3) culturing said T H -cells with a source of Timothy grass antigen B or cross reactive grass antigen, (4) separating T-helper factor, T HF , from said culture, (5) immunizing a second animal species with said T-helper factor, T HF , (6) collecting sera from said immunized second animal species, and (7) separating said anti-T HF antibody therefrom.
Claim 9
Independent Claim
139 words
A method of preparing an anti-idiotypic antibody directed against (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific allergenic T-helper factor, T HF , said anti-idiotypic antibody, anti-T HF , being specifically reactive with the idiotypic determinants on said T HF and (1) antigen B- and (2) cross reactive grass antigen-specific T and B cells comprising: (1) immunizing a first animal species with photooxidized antigen B, (2) collecting T H - cells from said immunized first animal species, (3) culturing said T H -cells with a source of Timothy grass antigen B or cross reactive grass antigen, (4) separating T-helper factor, T HF , from said culture, (5) immunizing a second animal species with said T-helper factor, T HF , (6) collecting sera from said immunized second animal species, and (7) separating said anti-T HF antibody therefrom.
Claim 10
Dependent Claim
17 words
The method of claim 6 wherein said second animal species is different from said first animal species.
Claim 11
Dependent Claim
19 words
The method of claim 6 wherein said first animal species is mouse and said second animal species is rabbit.
Claim 12
Dependent Claim
20 words
The method of claim 6 wherein said T H -cells are collected from the spleen of said first animal species.
Claim 13
Dependent Claim
19 words
The method of claim 6 wherein said first animal species is immunized with photooxidized antigen B adsorbed on alum.
Claim 14
Dependent Claim
25 words
The method of claim 6 wherein said culture medium containing T HF is centrifuged and T HF isolated from the cell-free supernatant by affinity chromatography.
Claim 15
Dependent Claim
16 words
The method of claim 6 wherein said anti-T HF is isolated from sera by affinity chromatography.
Claim 16
Dependent Claim
5 words
The antibody, anti-T HF .
Claim 17
Dependent Claim
5 words
The antibody, anti-T HF .
Claim 18
Dependent Claim
57 words
A method of producing (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific T suppressor cells, T S , comprising: (1) culturing the antibody of claim 5 or 13 with normal cells of an animal to induce the formation of T S cells in said culture and (2) separating said T S from said culture.
Claim 19
Dependent Claim
12 words
The method of claim 14 wherein said normal cells comprise spleen cells.
Claim 20
Dependent Claim
14 words
The method of claim 14 wherein said normal cells comprise human peripheral blood lymphocytes.
Claim 21
Independent Claim
37 words
A method of producing Timothy grass antigen B- and crossreactive grass antigen-specific T suppressor cells, T S , comprising: 1. immunizing an animal species with anti-IgE id and, 2. collecting T s cells from said animal species.
Claim 22
Independent Claim
37 words
A method of producing Timothy grass antigen B- and crossreactive grass antigen-specific T suppressor cells, T S , comprising: 1. immunizing an animal species with anti-IgE id and, 2. collecting T s cells from said animal species.
Claim 23
Dependent Claim
21 words
The method of claim 17 wherein said T S - cells are derived from the spleen of said immunized animal species.
Claim 24
Independent Claim
37 words
A method of producing (1) Timothy grass antigen B- and (2) cross reactive grass-antigen specific suppressor T-cell factor, T SF , by repeatedly freezing and thawing Ts cells and separating said Ts cells from T SF .
Claim 25
Independent Claim
37 words
A method of producing (1) Timothy grass antigen B- and (2) cross reactive grass-antigen specific suppressor T-cell factor, T SF , by repeatedly freezing and thawing Ts cells and separating said Ts cells from T SF .
Claim 26
Dependent Claim
14 words
The method of claim 19 wherein said T SF is isolated by affinity chromatography.
Claim 27
Independent Claim
44 words
A method for producing (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific suppression T-cell factor, T SF , comprising: (1) culturing T suppressor cells, T S , with antigen-IgE id , and (2) separating T SF from said cell containing culture.
Claim 28
Independent Claim
44 words
A method for producing (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific suppression T-cell factor, T SF , comprising: (1) culturing T suppressor cells, T S , with antigen-IgE id , and (2) separating T SF from said cell containing culture.
Claim 29
Dependent Claim
29 words
The method of claim 21 wherein said T SF containing medium is centrifuged to obtain a cell-free supernatant and T SF is recovered from said supernatant by affinity chromatography.
Claim 30
Independent Claim
97 words
A method of fractionating T SF into separate factors, T SF .sbsb.1 and T SF .sbsb.2, comprising the steps of: (1) subjecting T SF to affinity chromatography on an adsorbent comprising Timothy grass antigen D 1 on an inert carrier substrate; (2) collecting the non-adsorbing fraction; (3) eluting T SF .sbsb.1 adsorbed on said adsorbent and separating T SF .sbsb.1 from the eluant; (4) subjecting the non-adherent fraction to affinity chromotagraphy on an adsorbent comprising T SF .sbsb.1 on an inert carrier substrate, eluting the T SF .sbsb.2 therefrom and separating T SF .sbsb.2 from the eluant.
Claim 31
Independent Claim
97 words
A method of fractionating T SF into separate factors, T SF .sbsb.1 and T SF .sbsb.2, comprising the steps of: (1) subjecting T SF to affinity chromatography on an adsorbent comprising Timothy grass antigen D 1 on an inert carrier substrate; (2) collecting the non-adsorbing fraction; (3) eluting T SF .sbsb.1 adsorbed on said adsorbent and separating T SF .sbsb.1 from the eluant; (4) subjecting the non-adherent fraction to affinity chromotagraphy on an adsorbent comprising T SF .sbsb.1 on an inert carrier substrate, eluting the T SF .sbsb.2 therefrom and separating T SF .sbsb.2 from the eluant.
Claim 32
Dependent Claim
6 words
The protein fraction T SF .sbsb.2.
Claim 33
Dependent Claim
6 words
The protein fraction T SF .sbsb.2.
Claim 34
Independent Claim
46 words
A method of producing Timothy grass antigen B-and cross reactive grass antigen-specific T suppressor cells, T S , comprising: (1) culturing T SF .sbsb.1 or T SF .sbsb.2 with normal cells of an animal species, and (2) separating T S from said normal cell containing culture.
Claim 35
Independent Claim
46 words
A method of producing Timothy grass antigen B-and cross reactive grass antigen-specific T suppressor cells, T S , comprising: (1) culturing T SF .sbsb.1 or T SF .sbsb.2 with normal cells of an animal species, and (2) separating T S from said normal cell containing culture.
Claim 36
Dependent Claim
48 words
A pharmaceutical composition in unit dosage form for suppressing the allergic response of an animal sensitive to Timothy grass antigen B or a cross reactive grass antigen comprising an anti-allergic response effective amount of the product of claims 5, 13, or 24 and a pharmaceutically acceptable carrier therefor.
Claim 37
Independent Claim
39 words
A method for suppressing the allergic response of an animal sensitive to Timothy grass antigen B or a cross reactive grass antigen comprising administering thereto an anti-allergic response effective amount of the product of claims 5, 13, or 24.
Claim 38
Dependent Claim
46 words
A pharmaceutical composition in unit dosage form for suppressing the allergic response of an animal sensitive to Timothy grass antigen B or a cross reactive grass antigen comprising an anti-allergic response effective amount of the product when made by the process of claims 19 or 23.
Claim 39
Independent Claim
43 words
A method for suppressing the allergic response of an animal sensitive to Timothy grass antigen B or a cross reactive grass antigen comprising administering thereto an anti-allergic response effective amount of the product when made by the process of claims 19 or 23.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-10-07 | 1989-04-08 | 1989-10-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-10-07 | 1993-04-08 | 1993-10-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-10-07 | 1997-04-08 | 1997-10-08 | $7700.00 | Window Closed / Lapsed |