US4683203A
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US
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Immobilized enzymes, processes for preparing same, and use thereof
Assignee: Redco NV SA
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Filing: 1985-03-27
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Granted: 1987-07-28
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Expires: 2005-03-27
Abstract
Abstract Immobilized enzymes covalently bound to an inorganic carrier by an amino group through a bifunctional spacer have improved properties when the carrier is formed of amorphous, approximately spherical silica particles obtained from synthetic calcium silicate. The silica particles have an average particle size of from 15 to 80 mu m, an apparent particle volume of from 1.3 to 3 cm3/g, and a specific surface area of from 250 to 800 m2/g.
Inventors
- 👤 Octavian Anton
- 👤 Robert Crichton
- 👤 Jean-Pierre Lenders
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 09:04
CPC Classifications
B; B01; B01J; B01J23/00; B01J23/38; B01J23/40; B01J21/00; B01J21/06; B01J21/08; B01J23/42; B01J23/46; B01J23/462; B01J23/70; B01J23/74; B01J23/755; C; C01; C01B; C01B33/00; C01B33/113; C01B33/12; C01B33/18; C01B33/187; C07; C07C; C07C209/00; C07C209/30; C07C209/32; C07C209/36; C07C4/00; C07C4/02; C07C4/06; C07C5/00; C07C5/02; C07C5/10; C07C5/32; C07C5/373; C07C5/393; C07C5/41; C07C5/415; C07C5/417; C12; C12N; C12N11/00; C12N11/14; C12P; C12P19/00; C12P19/14; C12P19/24; B01J35/00; B01J35/40; B01J35/60; B01J35/61; B01J35/615; B01J35/617; B01J35/63; B01J35/638; C01P; C01P2002/00; C01P2002/02; C01P2004/00; C01P2004/01; C01P2004/03; C01P2004/30; C01P2004/32; C01P2006/00; C01P2006/12; C01P2006/80; C07C2521/00; C07C2521/06; C07C2521/08; C07C2523/00; C07C2523/38; C07C2523/40; C07C2523/42; C07C2523/44; C07C2523/46; C07C2523/70; C07C2523/74; C07C2523/755; C07C2601/00; C07C2601/12; C07C2601/14
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
87 words
An immobilized enzyme covalently bound to an inorganic carrier by an amino group through a bifunctional spacer, said carrier consisting of amorphous, approximately spherical silica particles obtained by the acidic hydrolysis of an approximately spherical synthetic calcium silicate having a particle size of from 20 to 120 μm, said silica particles having an average particle size of from 15 to 80 μm, an apparent particle volume of from 1.3 to 3 cm 3 /g, and a specific surface area of from 250 to 800 m 2 /g.
Claim 2
Dependent Claim
87 words
An immobilized enzyme covalently bound to an inorganic carrier by an amino group through a bifunctional spacer, said carrier consisting of amorphous, approximately spherical silica particles obtained by the acidic hydrolysis of an approximately spherical synthetic calcium silicate having a particle size of from 20 to 120 μm, said silica particles having an average particle size of from 15 to 80 μm, an apparent particle volume of from 1.3 to 3 cm 3 /g, and a specific surface area of from 250 to 800 m 2 /g.
Claim 3
Dependent Claim
18 words
The immobilized enzyme of claim 1, wherein said carrier consists of silica particles treated with 3-aminopropyl triethoxysilane (APTS).
Claim 4
Dependent Claim
16 words
The immobilized enzyme of claim 2, wherein said carrier consists of silica particles treated with glutaraldehyde.
Claim 5
Dependent Claim
108 words
A process for the preparation of an immobilized enzyme covalently bound to an inorganic carrier by an amino group through a bifunctional spacer, comprising (a) providing amorphous, approximately spherical silica particles obtained by the acidic hydrolysis of an approximately spherical synthetic calcium silicate having a particle size of from 20 to 120 μm, said silica particles having an average particle size of from 15 to 80 μm, an apparent particle volume of from 1.3 to 3 cm 3 /g, and a specific surface area of from 250 to 800 m 2 /g; and (b) reacting the particles of step (a) with 3-aminopropyl triethoxysilane (APTS), glutaraldehyde, and an enzyme.
Claim 6
Dependent Claim
108 words
A process for the preparation of an immobilized enzyme covalently bound to an inorganic carrier by an amino group through a bifunctional spacer, comprising (a) providing amorphous, approximately spherical silica particles obtained by the acidic hydrolysis of an approximately spherical synthetic calcium silicate having a particle size of from 20 to 120 μm, said silica particles having an average particle size of from 15 to 80 μm, an apparent particle volume of from 1.3 to 3 cm 3 /g, and a specific surface area of from 250 to 800 m 2 /g; and (b) reacting the particles of step (a) with 3-aminopropyl triethoxysilane (APTS), glutaraldehyde, and an enzyme.
Claim 7
Dependent Claim
12 words
The process of claim 4, wherein said enzyme is cellobiase or glucose-isomerase.
Claim 8
Independent Claim
108 words
A method for preparing glucose from cellobiose comprising providing immobilized cellobiase covalently bound to an inorganic carrier by an amino group through a bifunctional spacer, said carrier consisting of amorphous, approximately spherical silica particles obtained by the acidic hydrolysis of an approximately spherical synthetic calcium silicate having a particle size of from 20 to 120 μm, said silica particles having an average particle size of from 15 to 80 μm, an apparent particle volume of from 1.3 to 3 cm 3 /g, and a specific surface area of from 250 to 800 m 2 /g, subjecting said immobilized cellobiase to treatment to provide glucose, and recovering said glucose.
Claim 9
Independent Claim
108 words
A method for preparing glucose from cellobiose comprising providing immobilized cellobiase covalently bound to an inorganic carrier by an amino group through a bifunctional spacer, said carrier consisting of amorphous, approximately spherical silica particles obtained by the acidic hydrolysis of an approximately spherical synthetic calcium silicate having a particle size of from 20 to 120 μm, said silica particles having an average particle size of from 15 to 80 μm, an apparent particle volume of from 1.3 to 3 cm 3 /g, and a specific surface area of from 250 to 800 m 2 /g, subjecting said immobilized cellobiase to treatment to provide glucose, and recovering said glucose.
Claim 10
Independent Claim
107 words
A method for preparing fructose from glucose comprising providing immobilized glucose-isomerase covalently bound to an inorganic carrier by an amino group through a bifunctional spacer, said carrier consisting of amorphous, approximately spherical silica particles obtained by the acidic hydrolysis of an approximately spherical synthetic calcium silicate having a particle size of from 20 to 120 μm, said silica particles having an average particle size of from 15 to 80 μm, an apparent particle volume of from 1.3 to 3 cm 3 /g, and a specific surface area of from 250 to 800 m 2 /g, subjecting said immobilized glucose-isomerase treatment to provide fructose, and recovering said fructose.
Claim 11
Independent Claim
107 words
A method for preparing fructose from glucose comprising providing immobilized glucose-isomerase covalently bound to an inorganic carrier by an amino group through a bifunctional spacer, said carrier consisting of amorphous, approximately spherical silica particles obtained by the acidic hydrolysis of an approximately spherical synthetic calcium silicate having a particle size of from 20 to 120 μm, said silica particles having an average particle size of from 15 to 80 μm, an apparent particle volume of from 1.3 to 3 cm 3 /g, and a specific surface area of from 250 to 800 m 2 /g, subjecting said immobilized glucose-isomerase treatment to provide fructose, and recovering said fructose.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-07-27 | 1991-01-26 | 1991-07-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-07-27 | 1995-01-26 | 1995-07-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-07-27 | 1999-01-26 | 1999-07-28 | $7700.00 | Window Closed / Lapsed |