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US4677080A
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Rapid particle agglutination test for enterotoxigenic bacteria
Assignee: MISSOURI 227 UNIVERSITY HALL COLUMBIA MO 65201 A CORP OF, University of, Curators of
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Filing: 1984-05-15
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Granted: 1987-06-30
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Expires: 2004-05-15
Abstract
Abstract A rapid technique is disclosed for the identification of enteric bacteria which elaborate cholera-related heat-labile enterotoxin antigens which have the ability to cause diarrheal disease in man or animals. The invention includes a sensitized carrier particle for use as a reagent in an agglutination test for heat-labile enterotoxins and the use of said reagent in immunological determinations.
Inventors
- 👤 Richard A. Finkelstein
- 👤 Yang Zhengshi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 02:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56911; G01N33/56938; G01N33/56916; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/811
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
106 words
In an agglutination test, which test comprises sensitizing microscopic carrier particles with an antibody to a cholera-related heat-labile enterotoxin antigen and bringing this combination into contact with a specimen suspected to contain the corresponding antigen and observing the thus formed mixture for evidence of the formation of a macroscopic insoluble complex which indicates a positive reaction, the improvement in the method which comprises isolating colonies of the specimen from a primary culture, suspending the colonies in a medium, pretreating the colonies in the medium to liberate the enterotoxin from the cells, precipitating the cells and testing the supernatant containing the heat-labile enterotoxin liberated by the cells.
Claim 2
Dependent Claim
106 words
In an agglutination test, which test comprises sensitizing microscopic carrier particles with an antibody to a cholera-related heat-labile enterotoxin antigen and bringing this combination into contact with a specimen suspected to contain the corresponding antigen and observing the thus formed mixture for evidence of the formation of a macroscopic insoluble complex which indicates a positive reaction, the improvement in the method which comprises isolating colonies of the specimen from a primary culture, suspending the colonies in a medium, pretreating the colonies in the medium to liberate the enterotoxin from the cells, precipitating the cells and testing the supernatant containing the heat-labile enterotoxin liberated by the cells.
Claim 3
Dependent Claim
10 words
The method of claim 1 wherein the antibodies are immunopurified.
Claim 4
Dependent Claim
37 words
The method of claim 2 wherein the colonies isolated from the primary culture and suspended in the medium are pretreated in the medium with an antibiotic which alters the permeability of the cell walls releasing the enterotoxins.
Claim 5
Dependent Claim
10 words
The method of claim 3 wherein the antibiotic is polymyxin.
Claim 6
Dependent Claim
25 words
The method of claim 3 wherein the particles are latex and wherein the antibodies are directed to a human heat-labile enterotoxin antigen of Escherichia coli.
Claim 7
Dependent Claim
26 words
The method of claim 5 wherein the particles have a size in the range of about 0.5 to 1.5 micron and wherein the antibodies are immunopurified.
Claim 8
Dependent Claim
25 words
The method of claim 3 wherein the particles are latex and wherein the antibodies are directed to a procine heat-labile enterotoxin antigen of Escherichia coli.
Claim 9
Dependent Claim
26 words
The method of claim 7 wherein the particles have a size in the range of about 0.5 to 1.5 micron and wherein the antibodies are immunopurified.
Claim 10
Independent Claim
14 words
The method of claims 5 or 7 wherein the antibody is as goat antibody.
Claim 11
Dependent Claim
16 words
The method of claim 3 wherein the antibodies are homologous to the antigen to be detected.
References Cited (Backward Citations)
US4533630A
Cited
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