Patent US4675283A successfully refreshed from Google Patents.
US4675283A
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Detection and isolation of homologous, repeated and amplified nucleic acid sequences
Assignee: Massachusetts Institute of Technology
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Filing: 1984-07-19
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Granted: 1987-06-23
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Expires: 2004-07-19
Abstract
Abstract A novel method for detecting and isolating DNA sequences commonly held by different DNA preparations or repeated or amplified within a complex genome has been provided. The DNA preparations of interest are digested with the same restriction enzyme and a portion of at least one preparation is labeled with 32 P. The labeled and unlabeled DNA preparations are combined and electrophoresed in an agarose gel. Following electrophoresis, the DNA is denatured in situ and allowed to reanneal within the gel so that homologous DNA sequences present within restriction fragments of the same size can reanneal. After reannealing, unhybridized single-stranded DNA is digested in situ followed by detection of the reannealed DNA by autoradiography. When labeled and unlabeled DNAs are derived from different DNA preparations, only the restriction fragments commonly held by these two preparations are detected. When a restriction digest of total eukaryotic DNA is reassociated in the gel by this procedure, repeated restriction fragments are selectively detected. This approach permits detection of selectively amplified DNA sequences and identification of the DNA sequences that have been commonly amplified in different cell populations. Localized of homologous, repeated or amplified DNA fragments of interest within the gel permits size-purification of such fragments.
Inventors
- 👤 Igor Roninson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 09:23
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6827; C12Q1/683; C12Q1/6809; C12Q1/6816; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
198 words
A method for detecting and isolating individual nucleic acid sequences commonly held by a plurality of different double stranded nucleic acids of interest comprising the steps of: obtaining a plurality of different double stranded nucleic acids of interest; converting said double stranded nucleic acids into a plurality of double stranded nucleic acid fragments with an endonuclease composition containing at least one endonuclease, said endonuclease composition being the same for each sample of nucleic acid of interest; introducing an identifiable label into one of the nucleic acids of interest; combining said labelled nucleic acid with at least one other unlabelled nucleic acid of interest to form a mixture; subjecting said mixture of double-stranded nucleic acid fragments to gel electrophoresis whereby a plurality of separated, double-stranded nucleic acid molecules are individually localized in the gel; denaturing in said gel said separated nucleic acid molecules to form single-stranded molecules; renaturing in said gel at least a portion of said single-stranded nucleic acid molecules to form reannealed doublestranded molecules; eliminating such single-stranded nucleic acid molecules as remains in said gel; and detecting said identifiable label within said reannealed double-stranded nucleic acid molecules whereby the individual nucleic acid sequences commonly held are isolated.
Claim 2
Independent Claim
198 words
A method for detecting and isolating individual nucleic acid sequences commonly held by a plurality of different double stranded nucleic acids of interest comprising the steps of: obtaining a plurality of different double stranded nucleic acids of interest; converting said double stranded nucleic acids into a plurality of double stranded nucleic acid fragments with an endonuclease composition containing at least one endonuclease, said endonuclease composition being the same for each sample of nucleic acid of interest; introducing an identifiable label into one of the nucleic acids of interest; combining said labelled nucleic acid with at least one other unlabelled nucleic acid of interest to form a mixture; subjecting said mixture of double-stranded nucleic acid fragments to gel electrophoresis whereby a plurality of separated, double-stranded nucleic acid molecules are individually localized in the gel; denaturing in said gel said separated nucleic acid molecules to form single-stranded molecules; renaturing in said gel at least a portion of said single-stranded nucleic acid molecules to form reannealed doublestranded molecules; eliminating such single-stranded nucleic acid molecules as remains in said gel; and detecting said identifiable label within said reannealed double-stranded nucleic acid molecules whereby the individual nucleic acid sequences commonly held are isolated.
Claim 3
Independent Claim
198 words
A method for detecting and isolating individual nucleic acid sequences repeated seriatim within a doublestranded nucleic acid of interest comprising the steps of: obtaining a double stranded nucleic acid of interest; converting said double-stranded nucleic acids into a plurality of double-stranded nucleic acid fragments with an endonuclease composition containing at least one endonuclease, introducing an identifiable label into at least a portion of said nucleic acid fragments said portion having the same composition of different nucleic acid fragments as the nucleic acid fragments obtained from said converting step; combining said labelled nucleic acid fragments of interest with unlabelled nucleic acid fragments of interest to form a mixture; subjecting said double-stranded nucleic acid fragments to gel electrophoresis whereby a plurality of separated, double-stranded nucleic acid molecules are individually localized in the gel; denaturing in said gel said separated nucleic acid molecules to form single-stranded nucleic acid molecules; renaturing in said gel at least a portion of said single-stranded nucleic acid molecules to form reannealed doublestranded molecules; eliminating such single-stranded nucleic acid molecules as remains in said gel; and detecting said identifiable label within said reannealed double-stranded nucleic acid molecules whereby the individual nucleic acid sequence repeated seriatum are isolated.
Claim 4
Independent Claim
198 words
A method for detecting and isolating individual nucleic acid sequences repeated seriatim within a doublestranded nucleic acid of interest comprising the steps of: obtaining a double stranded nucleic acid of interest; converting said double-stranded nucleic acids into a plurality of double-stranded nucleic acid fragments with an endonuclease composition containing at least one endonuclease, introducing an identifiable label into at least a portion of said nucleic acid fragments said portion having the same composition of different nucleic acid fragments as the nucleic acid fragments obtained from said converting step; combining said labelled nucleic acid fragments of interest with unlabelled nucleic acid fragments of interest to form a mixture; subjecting said double-stranded nucleic acid fragments to gel electrophoresis whereby a plurality of separated, double-stranded nucleic acid molecules are individually localized in the gel; denaturing in said gel said separated nucleic acid molecules to form single-stranded nucleic acid molecules; renaturing in said gel at least a portion of said single-stranded nucleic acid molecules to form reannealed doublestranded molecules; eliminating such single-stranded nucleic acid molecules as remains in said gel; and detecting said identifiable label within said reannealed double-stranded nucleic acid molecules whereby the individual nucleic acid sequence repeated seriatum are isolated.
Claim 5
Dependent Claim
70 words
The method as recited in claim 12 or 13 further comprising: excising that portion of said gel containing individually localized, double-stranded nucleic acid molecules; denaturing said separated nucleic acids within said excised portion of said gel into single-stranded molecules; renaturing in said excised gel at least a portion of said single-stranded nucleic acid molecules into double-stranded molecules; and disrupting such single-stranded nucleic acid molecules as remains in said excised gel.
Claim 6
Dependent Claim
80 words
The method as recited in claim 12 or 13 further comprising: excising that portion of said gel containing an individually localized, separated, double-stranded nucleic acid molecules; denaturing the nucleic acids within said excised portion of said gel into single-stranded nucleic acid molecules; renaturing at least a portion of said single-stranded nucleic acid molecules into double-stranded nucleic acid molecules; and eliminating such single-stranded nucleic acid molecules as remains in said excised gel by selectively cloning said reannealed double-stranded nucleic acid molecules.
Claim 7
Dependent Claim
62 words
The method of claim 1 or 2 which includes the additional steps of: purifying said reannealed double-stranded molecules by excising the portion of said gel containing such reannealed molecules and eluting such reannealed molecules from said gel; cloning said purified molecules as recombinant DNA clones; and testing individual recombinant DNA clones for the presence of the isolated nucleic acid sequences of interest.
Claim 8
Dependent Claim
17 words
The method as recited in claim 1 or 2 wherein the nucleic acid sequences comprise deoxyribonucleic acids.
Claim 9
Dependent Claim
17 words
The method as recited in claim 1 or 2 wherein the nucleic acid sequences comprise ribonucleic acids.
Claim 10
Dependent Claim
16 words
The method as recited in claim 1 or 2 wherein said identifiable label is a radionuclide.
Claim 11
Dependent Claim
19 words
The method as recited in claim 1 or 2 wherein said nucleic acid molecules are denatured prior to separation.
Claim 12
Dependent Claim
28 words
The method as recited in claim 1 or 2 wherein said eliminating step comprises combining said reannealed double-stranded molecules with a nuclease specific for single-stranded nucleic acid sequences.
Claim 13
Dependent Claim
37 words
The method as recited in claim 1 or 2 wherein said steps of denaturation, renaturation and elimination of single-stranded nucleic acid sequences are repeated prior to said detection of the identifiable label within said reannealed double-stranded molecules.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-22 | 1990-12-22 | 1991-06-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-22 | 1994-12-22 | 1995-06-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-22 | 1998-12-22 | 1999-06-23 | $7700.00 | Window Closed / Lapsed |