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US4673635A
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Triphenyl methane derivatives and method of quantitatively measuring an oxidative substance
Assignee: Fujifilm Wako Pure Chemical Corp
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Filing: 1984-12-19
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Granted: 1987-06-16
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Expires: 2004-12-19
Abstract
Abstract Disclosed herein is a novel compound which is excellent in solubility to water around a neutral range, stable in an aqueous solution state for a long period of time, and high in coloring sensitivity with a stable coloring development, and is represented by the general formula (I): ##STR1## wherein R 1 , R 2 , R 3 and R 4 , which may be the same as or different from one another, represent lower alkyl groups, and X 1 and X 2 both represent --O(CH 2 ) n SO 3 M in which M is a hydrogen atom, an alkali metal ion or NH 4 + , and n is an integer of 2-4, or either of X 1 and X 2 represents --O(CH 2 ) n SO 3 M in which M and n are the same meanings as given above and the other represents a hydrogen atom. This reagent is hardly susceptible of the influence of the serum components such as hemoglobin, bilirubin and so on due to its color having absorption on a longer wavelength side. Disclosed is also a method of quantitatively measuring an oxidative substance, particularly, hydrogen peroxide, by using this color test reagent.
Inventors
- 👤 Kazuhiko Yamanishi
- 👤 Akinori Shintani
- 👤 Satoru Okajima
- 👤 Toshiro Hanada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 16:02
CPC Classifications
B; B41; B41M; B41M5/00; B41M5/124; B41M5/132; B41M5/136; B41M5/1366; C; C09; C09B; C09B11/00; C09B11/04; C09B11/10; C09B11/12; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/40; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/20; Y10T436/206664
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
125 words
A method for quantitatively measuring an oxidative substance by: measuring an oxidative substance using, as a coloring component, a triphenyl methane derivative represented by the formula (I): ##STR4## wherein R 1 , R 2 , R 3 and R 4 , independent of each other, represent lower alkyl groups having from 1-3 carbon atoms, and (i) X 1 and X 2 each represent --O(CH 2 ) n SO 3 M in which M is a hydrogen atom, an alkali metal ion or NH 4 , and n is an integer of 2-4, or (ii) either X 1 or X 2 represents --O(CH 2 ) n SO 3 M, M and n having the aforesaid meanings as given above, and the other represents a hydrogen atom.
Claim 2
Independent Claim
125 words
A method for quantitatively measuring an oxidative substance by: measuring an oxidative substance using, as a coloring component, a triphenyl methane derivative represented by the formula (I): ##STR4## wherein R 1 , R 2 , R 3 and R 4 , independent of each other, represent lower alkyl groups having from 1-3 carbon atoms, and (i) X 1 and X 2 each represent --O(CH 2 ) n SO 3 M in which M is a hydrogen atom, an alkali metal ion or NH 4 , and n is an integer of 2-4, or (ii) either X 1 or X 2 represents --O(CH 2 ) n SO 3 M, M and n having the aforesaid meanings as given above, and the other represents a hydrogen atom.
Claim 3
Dependent Claim
94 words
The method according to claim 1 wherein the method is conducted using a sample, said method further comprising: providing a color test liquid comprising a phosphate buffer solution, 3,4-disodium sulfopropoxybenzaldehyde, peroxidase, and at least one surfactant; admixing a selected aliquot of said color test liquid with a selected aliquot of said sample whereby a mixture is obtained and incubating said mixture; and determining the concentration of hydrogen peroxide in said sample by first measuring the light absorbance of said sample at a selected wavelength with reference to a hydrogen peroxide standard solution calibration curve.
Claim 4
Dependent Claim
28 words
The method according to claim 1, wherein the coloring component is oxidized and colored in the presence of a peroxidase, and the color thus developed is colorimetrically determined.
Claim 5
Dependent Claim
13 words
The method according to claim 3, wherein the oxidative substance is hydrogen peroxide.
Claim 6
Dependent Claim
33 words
The method according to claim 4, wherein in said method a sample from a living organism is provided and said quantitative measurement is of a component in said sample from a living organism.
Claim 7
Dependent Claim
50 words
The method according to claim 5, wherein said quantitative measurement is of a substrate or enzyme activity in said sample wherein an oxidation enzyme acts upon the substrate or a substance is produced through the enzyme reaction to generate hydrogen peroxide, and the thus generated hydrogen peroxide is quantitatively measured.
Claim 8
Dependent Claim
41 words
The method according to claim 5 wherein the component of said sample which is being analyzed is glucose, free cholesterol, whole cholesterol, high specific density liquid protein cholesterol, low specific density liquid protein cholesterol, trigylceride, phospholipid, uric acid or monoamine oxidase.
Claim 9
Dependent Claim
117 words
The method according to claim 5 wherein said sample is a serum sample and said component is monoamine oxidase, said method further comprising: (a) providing a serum sample; (b) preparing a substrate color test liquid comprising a substrate, uricase, 3,4-disodium sulfopropoxybenzaldehyde, a surfactant and a buffer solution; (c) adding a selected aliquot of the thus prepared substrate color test liquid to a selected aliquot of said serum sample to obtain a mixture and incubating said mixture; (d) mixing a reaction terminator liquid with the mixture of the aforesaid step; and colorimetrically determining the serum monoamine oxidase activity by measuring the absorbance characteristics of the mixture of step (d) at 620 nm against a monoamine oxidase reagent control.
Claim 10
Dependent Claim
113 words
The method according to claim 5 wherein said sample is a serum sample and said component is free cholesterol, said process further comprising: (a) providing a blood serum sample; (b) preparing a color test liquid comprising a phosphate buffer solution, 3,4-disodium sulfopropoxybenzaldehyde, uricase, cholesterol oxidase, peroxidase and at least one surfactant; (c) admixing a specific aliquot of said color test liquid with a selected aliquot of said serum sample whereby a mixture is obtained and incubating said mixture; (d) colorimetrically determining the concentration of the cholesterol in said serum sample by determining the absorbance from said mixture of step (c) at 620 nm with respect to a cholesterol standard reagent as a control.
References Cited (Backward Citations)
US4066408A
Cited
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