Patent US4672034A successfully refreshed from Google Patents.
US4672034A
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Oxidative enzyme-catalyzed crosslinking of beet pectins
Assignee: Institut National de la Recherche Agronomique
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Filing: 1984-04-24
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Granted: 1987-06-09
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Expires: 2004-04-24
Abstract
Abstract Beet pectin is crosslinked with an oxidizing system containing an oxidizing agent and an enzyme such as peroxidase that uses the oxidizing agent as a substrate. The crosslinked beet pectin is useful as a thickener or gelling agent in food, cosmetic and pharmaceutical products.
Inventors
- 👤 Franciscus M. Rombouts
- 👤 Jean-Francois Thibault
- 👤 Christiane Mercier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:48
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/0006; C08B37/0045; C12; C12P; C12P19/00; C12P19/04
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
73 words
A process for modifying beet pectins comprising reacting a beet pectin starting solution in a reaction medium with an oxidizing system, said oxidizing system comprising at least one oxidizing agent and a peroxidase which has said oxidizing agent as a substrate wherein the oxidizing system is employed in an amount effective and for a time sufficient to crosslink the pectin to form a modified beet pectin solution of increased viscosity or a gel.
Claim 2
Dependent Claim
73 words
A process for modifying beet pectins comprising reacting a beet pectin starting solution in a reaction medium with an oxidizing system, said oxidizing system comprising at least one oxidizing agent and a peroxidase which has said oxidizing agent as a substrate wherein the oxidizing system is employed in an amount effective and for a time sufficient to crosslink the pectin to form a modified beet pectin solution of increased viscosity or a gel.
Claim 3
Dependent Claim
14 words
A process as claimed in claim 1, wherein the oxidizing agent is hydrogen peroxide.
Claim 4
Dependent Claim
73 words
A process as claimed in claim 2, wherein said starting solution is an aqueous pectin solution, and said process is carried out by ( 1) adding to the aqueous pectin solution an amount of peroxidase of from 0.01 mg to 1 mg per 1 g of pectin; and (2) thereafter adding an aqueous hydrogen peroxide solution containing from 10 μmoles to 1 mmole of H 2 O 2 per 1 g of pectin.
Claim 5
Dependent Claim
45 words
A process as claimed in claim 3, wherein after crosslinking, the modified pectin is precipitated by adding to the reaction medium an agent selected from the group consisting of a solvent and a salting-out agent, and recovering and drying the precipitate to form a powder.
Claim 6
Dependent Claim
32 words
A process as claimed in claim 3, wherein about 0.1 mg of catalase per about 1 mmole of H 2 O 2 is added to decompose unreacted H 2 O 2 .
Claim 7
Dependent Claim
17 words
A process according to claim 1 wherein a modified beet pectin solution of increased viscosity is formed.
Claim 8
Dependent Claim
17 words
A process according to claim 2 wherein a modified beet pectin solution of increased viscosity is formed.
Claim 9
Dependent Claim
49 words
A process according to claim 3, wherein the pectin starting solution has a pectin concentration of less than 1.5 g/100 ml and the oxidizing system comprises from 0.3 to 0.6 mg of peroxidase and from 13 to 36 μmoles of H 2 O 2 per 1 g of pectin.
Claim 10
Dependent Claim
49 words
A process according to claim 4, wherein the pectin starting solution has a pectin concentration of less than 1.5 g/100 ml and the oxidizing system comprises from 0.3 to 0.6 mg of peroxidase and from 13 to 36 μmoles of H 2 O 2 per 1 g of pectin.
Claim 11
Dependent Claim
11 words
A process according to claim 1, wherein a gel is formed.
Claim 12
Dependent Claim
49 words
A process according to claim 2, wherein the pectin starting solution has a pectin concentration of greater than 2 g/100 ml and the oxidizing system comprises from 0.3 to 0.6 mg of peroxidase and from 13 to 36 μmoles of H 2 O 2 per 1 g of pectin.
Claim 13
Dependent Claim
49 words
A process according to claim 2 wherein the pectin starting solution has a pectin concentration of greater than 0.3 g/100 ml and the oxidizing system comprises from 0.03 to 0.1 mg of peroxidase and from 0.25 to 0.1 mmole of H 2 O 2 per 1 g of pectin.
Claim 14
Dependent Claim
18 words
A process according to claim 4 wherein the powder is mixed with water to form an aqueous gel.
Claim 15
Dependent Claim
14 words
A water-soluble modified beet pectin prepared by the process as claimed in claim 6.
Claim 16
Dependent Claim
14 words
A water-soluble modified beet pectin prepared by the process as claimed in claim 7.
Claim 17
Dependent Claim
13 words
A water-soluble modified pectin prepared by the process as claimed in claim 8.
Claim 18
Dependent Claim
13 words
A modified pectin powder prepared by the process as claimed in claim 9.
Claim 19
Dependent Claim
21 words
A modified beet pectin capable of causing gelation of an aqueous medium produced by the process as claimed in claim 10.
Claim 20
Dependent Claim
21 words
A modified beet pectin capable of causing gelation of an aqueous medium, produced by the process as claimed in claim 11.
Claim 21
Dependent Claim
20 words
A modified beet pectin capable of causing gelation of aqueous medium produced by the process as claimed in claim 12.
Claim 22
Dependent Claim
10 words
An aqueous gel produced by the process of claim 13.
Claim 23
Dependent Claim
93 words
A process for thickening comprising the steps of reacting a beet pectin starting solution in a reaction medium with an oxidizing system, said oxidizing system comprising at least one oxidizing agent and a peroxidase which has the said oxidizing agent as a substrate wherein the oxidizing system is employed in an amount effective and for a time sufficient to crosslink the pectin to form an ungelled water-soluble modified beet pectin solution of increased viscosity, and adding the water-soluble modified beet pectin as a thickening agent to foodstuff products, cosmetics products or pharmaceutical products.
Claim 24
Dependent Claim
93 words
A process for thickening comprising the steps of reacting a beet pectin starting solution in a reaction medium with an oxidizing system, said oxidizing system comprising at least one oxidizing agent and a peroxidase which has the said oxidizing agent as a substrate wherein the oxidizing system is employed in an amount effective and for a time sufficient to crosslink the pectin to form an ungelled water-soluble modified beet pectin solution of increased viscosity, and adding the water-soluble modified beet pectin as a thickening agent to foodstuff products, cosmetics products or pharmaceutical products.
Claim 25
Dependent Claim
91 words
A process for gelling comprising the steps of reacting a beet pectin starting solution in a reaction medium with an oxidizing system, said oxidizing system comprising at least one oxidizing agent and a peroxidase which has the said oxidizing agent as a substrate wherein the oxidizing system is employed in an amount effective and for a time sufficient to crosslink the pectin to form a modified beet pectin and gel the reaction medium, and adding the modified beet pectin as a gelling agent to foodstuff products, cosmetics products or pharmaceutical products.
Claim 26
Dependent Claim
91 words
A process for gelling comprising the steps of reacting a beet pectin starting solution in a reaction medium with an oxidizing system, said oxidizing system comprising at least one oxidizing agent and a peroxidase which has the said oxidizing agent as a substrate wherein the oxidizing system is employed in an amount effective and for a time sufficient to crosslink the pectin to form a modified beet pectin and gel the reaction medium, and adding the modified beet pectin as a gelling agent to foodstuff products, cosmetics products or pharmaceutical products.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-08 | 1990-12-08 | 1991-06-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-08 | 1994-12-08 | 1995-06-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-08 | 1998-12-08 | 1999-06-09 | $7700.00 | Window Closed / Lapsed |