US4670394A
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US
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Isolation and culture of adrenal medullary endothelial cells producing blood clotting factor VIII:C
Assignee: UNITED SATEA OF AMERICA HEALTH AND HUMAN SERVICES THE, Secretary of, Department of
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Filing: 1984-11-16
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Granted: 1987-06-02
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Expires: 2004-11-16
Abstract
Abstract The present invention discloses a new line of endothelial cell of adrenal medullary origin capable of producing blood clotting Factor VIII:C. A method of isolating and culturing said cell line has also been disclosed. Factor VIII:C is useful in treating hemophilia.
Inventors
- 👤 Harvey B. Pollard
- 👤 Richard Ornberg
- 👤 Dipak Banerjee
- 👤 Moussa Youdim
- 👤 Peter Lelkes
- 👤 Eli Heldman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 21:44
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/069; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
17 words
A biologically pure culture of a profusely and continuously growing endothelial cell line of adrenal medullary origin.
Claim 2
Dependent Claim
17 words
A biologically pure culture of a profusely and continuously growing endothelial cell line of adrenal medullary origin.
Claim 3
Dependent Claim
18 words
The cell line of claim 1 having the properties of American Type Culture Collection accession number CRL 8659.
Claim 4
Dependent Claim
12 words
The cell line of claim 1 being of bovine adrenal medullary origin.
Claim 5
Dependent Claim
15 words
The cell line of claim 1 which produces blood clotting Factor VIII:C in recoverable amounts.
Claim 6
Dependent Claim
22 words
The cell line of claim 1 profusely and continuously growing on plastic surface in a nutrient culture medium unsupplemented with growth promoters.
Claim 7
Independent Claim
58 words
A method of in vitro culturing of adrenal medullary endothelial cells comprising the steps of dissociating adrenal medullary cells from an adrenal gland, and allowing said cells to proliferate in a medium and environment suitable for the growth of said cells to form recoverable amounts of continuously growing adrenal medullary cells, and then isolating therefrom said endothelial cells.
Claim 8
Independent Claim
58 words
A method of in vitro culturing of adrenal medullary endothelial cells comprising the steps of dissociating adrenal medullary cells from an adrenal gland, and allowing said cells to proliferate in a medium and environment suitable for the growth of said cells to form recoverable amounts of continuously growing adrenal medullary cells, and then isolating therefrom said endothelial cells.
Claim 9
Dependent Claim
14 words
The method of claim 6 wherein dissociating comprises treating said adrenal gland with collagenase.
Claim 10
Dependent Claim
35 words
The method of claim 6 wherein said medium comprises minimal essential medium supplemented with about 10% heat inactivated fetal calf serum, about 2mM glutamine and suitable amount of antibiotic to prevent growth of undesirable organisms.
Claim 11
Dependent Claim
21 words
The method of claim 8 wherein said cells are incubated at about 37° C. for about 3 hours in said medium.
Claim 12
Dependent Claim
16 words
The method of claim 6 wherein said endothelial cells produce blood-clotting Factor VIII:C in recoverable amounts.
References Cited (Backward Citations)
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US4446134A
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US4534972A
Cited
US4540573A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-01 | 1990-12-01 | 1991-06-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-01 | 1994-12-01 | 1995-06-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-01 | 1998-12-01 | 1999-06-02 | $7700.00 | Window Closed / Lapsed |