Patent US4668640A successfully refreshed from Google Patents.
US4668640A
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Fluorescence polarization immunoassay utilizing substituted carboxyfluoresceins
Assignee: Abbott Laboratories
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Filing: 1985-05-02
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Granted: 1987-05-26
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Expires: 2005-05-02
Abstract
Abstract This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescence polarization techniques to provide a means for determining the amount of a specific ligand present in a sample.
Inventors
- 👤 Chao-Huei J. Wang
- 👤 Stephen D. Stroupe
- 👤 Michael E. Jolley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 11:31
CPC Classifications
C; C07; C07D; C07D405/00; C07D405/02; C07D405/12; C07D311/00; C07D311/02; C07D311/78; C07D311/80; C07D311/82; C07D473/00; C07D473/02; C07D473/04; C07D473/06; C07D473/08; C07J; C07J41/00; C07J41/0005; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
103 words
A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula: ##STR33## wherein R is a ligand-analog having a single reaction primary or secondary amino group which is attached to a carbonyl carbon of a carboxyfluorescein moiety, wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody; and an antibody capable of specifically recognizing said ligand and said tracer; and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 2
Independent Claim
103 words
A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula: ##STR33## wherein R is a ligand-analog having a single reaction primary or secondary amino group which is attached to a carbonyl carbon of a carboxyfluorescein moiety, wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody; and an antibody capable of specifically recognizing said ligand and said tracer; and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 3
Dependent Claim
16 words
A method according to claim 1 wherein said ligand is a drug or a metabolite thereof.
Claim 4
Dependent Claim
27 words
A method according to claim 2 wherein the carbonyl group bonded to the R group is also bonded to the 4-or 5- position of the carboxyfluorescein moiety.
Claim 5
Dependent Claim
25 words
A method according to claim 3 wherein said drug is a steroid, hormone, antiasthamatic, antineoplastic, antiarrhythmic, anticonvulsant, antiarthritic, antidepressant, cardiac glycoside or a metabolite thereof.
Claim 6
Dependent Claim
19 words
A method according to claim 4 wherein R has a molecular weight within a range of 50 to 4000.
Claim 7
Dependent Claim
19 words
A method according to claim 5 wherein R has a molecular weight within a range of 100 to 2000.
Claim 8
Dependent Claim
12 words
A method according to claim 6 wherein said drug is an anticonvulsant.
Claim 9
Dependent Claim
12 words
A method according to claim 7 wherein said anticonvulsant drug is phenobarbital.
Claim 10
Dependent Claim
12 words
A method according to claim 7 wherein said anticonvulsant drug is phenytoin.
Claim 11
Dependent Claim
12 words
A method according to claim 7 wherein said anticonvulsant drug is primidone.
Claim 12
Dependent Claim
12 words
A method according to claim 6 wherein said drug is a steroid.
Claim 13
Dependent Claim
11 words
A method according to claim 10 wherein said steroid is digoxin.
Claim 14
Dependent Claim
12 words
A method according to claim 6 wherein said drug is an antiarrhythmic.
Claim 15
Dependent Claim
12 words
A method according to claim 13 wherein said antiarrhythmic drug is propranolol.
Claim 16
Dependent Claim
12 words
A method according to claim 6 wherein said drug is an antiasthmatic.
Claim 17
Dependent Claim
12 words
A method according to claim 15 wherein said antiasthmatic drug is theophylline.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-05-25 | 1990-11-24 | 1991-05-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-05-25 | 1994-11-24 | 1995-05-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-05-25 | 1998-11-24 | 1999-05-26 | $7700.00 | Window Closed / Lapsed |