US4656139A
Expired / Lapsed
US
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Method for preparing cells for blood analysis
Assignee: Sysmex Corp
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Filing: 1986-06-30
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Granted: 1987-04-07
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Expires: 2006-06-30
Abstract
Abstract A reagent for blood analysis comprises, as a lysing reagent for leukocyte count, tetradecyltrimethylammonium bromide and/or hexadecyltrimethylammonium chloride, dodecyltrimethylammonium chloride, and citric acid. This reagent is characterized by showing three-peaks fractionation of leukocytes in measuring by means of an automatic blood-analysis instrument. The lysing reagent is used together with a diluent comprising boric acid buffer solution, ethylenediaminetetraacetic acid and (2-pyridylthio-1-oxide) sodium.
Inventors
- 👤 Noriaki Matsuda
- 👤 Etsuro Shinkai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 18:11
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; G01N33/53; G01N33/569; G01N33/56966; G01N33/56972; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
59 words
A method for preparing cells for blood analysis comprising the stpes of: diluting a blood sample with a diluent comprising boric acid buffer solution, ethylenediaminetetraacetic acid and (2-pyridylthio-1-oxide) sodium, and treating the diluted blood with a lysing reagent comprising dodecyltrimethylammonium chloride; citric acid; and at least one member selected from the group consisting of tetradecyltrimethylammonium bromide and hexadecyltrimethylammonium chloride.
Claim 2
Independent Claim
59 words
A method for preparing cells for blood analysis comprising the stpes of: diluting a blood sample with a diluent comprising boric acid buffer solution, ethylenediaminetetraacetic acid and (2-pyridylthio-1-oxide) sodium, and treating the diluted blood with a lysing reagent comprising dodecyltrimethylammonium chloride; citric acid; and at least one member selected from the group consisting of tetradecyltrimethylammonium bromide and hexadecyltrimethylammonium chloride.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-04-06 | 1990-10-06 | 1991-04-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-06 | 1994-10-06 | 1995-04-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-06 | 1998-10-06 | 1999-04-07 | $7700.00 | Window Closed / Lapsed |