Patent US4656129A successfully refreshed from Google Patents.
US4656129A
Expired / Lapsed
US
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Assay for a ligand by use of supported binder and sac lysing agent
Assignee: Becton Dickinson and Co
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Filing: 1984-08-16
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Granted: 1987-04-07
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Expires: 2004-08-16
Abstract
Abstract In a homogeneous assay, binder supported on a solid support, having a sac lysing agent conjugated thereto, is contacted with analyte and tracer comprised of sacs containing a detectable marker. The sacs of the bound tracer portion are lysed to release marker to determine analyte in the sample.
Inventors
- 👤 Daniel B. Wagner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 03:56
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/5432; G01N33/58; G01N33/585; G01N33/586; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/975; Y10S436/00; Y10S436/829
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
103 words
An assay for an analyte in a sample, comprising: contacting a solid support with both tracer and analyte in a sample, said solid support prior to the contacting having supported thereon both a sac lysing agent and a binder for at least the analyte, said tracer comprising a ligand coupled to sacs having a detectable marker, said contacting producing a bound tracer phase and a free tracer phase, said bound tracer phase coming into contact with supported sac lysing agent to release detectable marker by lysing of sacs of bound tracer; and determining marker released from the sacs as a measure of analyte.
Claim 2
Dependent Claim
103 words
An assay for an analyte in a sample, comprising: contacting a solid support with both tracer and analyte in a sample, said solid support prior to the contacting having supported thereon both a sac lysing agent and a binder for at least the analyte, said tracer comprising a ligand coupled to sacs having a detectable marker, said contacting producing a bound tracer phase and a free tracer phase, said bound tracer phase coming into contact with supported sac lysing agent to release detectable marker by lysing of sacs of bound tracer; and determining marker released from the sacs as a measure of analyte.
Claim 3
Dependent Claim
20 words
The assay of claim 1 wherein the sac lysing agent and the binder are separately supported on the solid support.
Claim 4
Dependent Claim
22 words
The assay of claim 1 wherein the sac lysing agent is conjugated to the binder which is supported on the solid support.
Claim 5
Dependent Claim
12 words
The assay of claim 1 wherein the detectable marker is a chromogen.
Claim 6
Dependent Claim
11 words
The assay of claim 4 wherein the sac is a liposome.
Claim 7
Dependent Claim
11 words
The assay of claim 4 wherein the binder is an antibody.
Claim 8
Dependent Claim
20 words
The assay of claim 1 wherein the ligand portion of the tracer is bound by the binder for the analyte.
Claim 9
Dependent Claim
12 words
The assay of claim 1 wherein the lysing agent is an enzyme.
Claim 10
Dependent Claim
23 words
The assay of claim 1 wherein the marker released from the sacs is determined without separating the bound tracer portion from the sample.
Claim 11
Dependent Claim
10 words
The assay of claim 9 wherein the sample is serum.
Claim 12
Dependent Claim
12 words
The assay of claim 10 wherein the solid support is a tube.
Claim 13
Dependent Claim
25 words
The assay of claim 11 wherein the sac is a liposome containing a chromogen as a detectable marker and the lysing agent is an enzyme.
Claim 14
Dependent Claim
97 words
A reagent kit for use in an assay for analyte in a sample wherein the solid support is contacted with both tracer and analyte with the solid support prior to the contacting having supported thereon both the sac lysing agent and a binder for at least the analyte, comprising: a package, said package including a solid support having supported thereon a binder for at least the analyte and a sac lysing agent; and a tracer comprising a ligand coupled to sacs having a detectable marker, said sacs being capable of being lysed by said sac lysing agent.
Claim 15
Dependent Claim
97 words
A reagent kit for use in an assay for analyte in a sample wherein the solid support is contacted with both tracer and analyte with the solid support prior to the contacting having supported thereon both the sac lysing agent and a binder for at least the analyte, comprising: a package, said package including a solid support having supported thereon a binder for at least the analyte and a sac lysing agent; and a tracer comprising a ligand coupled to sacs having a detectable marker, said sacs being capable of being lysed by said sac lysing agent.
Claim 16
Dependent Claim
20 words
The kit of claim 13 wherein the sac lysing agent and the binder are separately supported on the solid support.
Claim 17
Dependent Claim
22 words
The kit of claim 13 wherein the sac lysing agent is conjugated to the binder which is supported on the solid support.
Claim 18
Dependent Claim
12 words
The kit of claim 13 wherein the detectable marker is a chromogen.
Claim 19
Dependent Claim
11 words
The kit of claim 16 wherein the sac is a liposome.
Claim 20
Dependent Claim
12 words
The kit of claim 16 wherein the lysing agent is an enzyme.
Claim 21
Dependent Claim
12 words
The kit of claim 13 wherein the solid support is a tube.
Claim 22
Dependent Claim
18 words
The kit of claim 13 wherein the sac is a liposome and the lysing agent is an enzyme.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-04-06 | 1990-10-06 | 1991-04-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-06 | 1994-10-06 | 1995-04-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-06 | 1998-10-06 | 1999-04-07 | $7700.00 | Window Closed / Lapsed |