US4650676A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antigens and monoclonal antibodies reactive against merozoites of Eimeria spp.
Assignee: Wyeth Holdings LLC
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Filing: 1984-03-19
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Granted: 1987-03-17
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Expires: 2004-03-19
Abstract
Abstract Monoclonal antibodies against merozoites and sporozoites of Eimeria tenella are obtained by use of hybridoma technology. Specific antigens for use as vaccines in the prevention and treatment of coccidiosis and hybridoma cultures producing monoclonal antibodies are described.
Inventors
- 👤 Robert H. Schenkel
- 👤 Pallaiah Thammana
- 👤 Rosie B. Wong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 08:19
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/20; C07K14/00; C07K14/435; C07K14/44; C07K14/455; A; A61; A61K; A61K39/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/947
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
57 words
An antigenic, immunogenic, proteinaceous vaccine for combatting Eimeria spp. infection in avians which comprises: an effective amount of Eimeria tenella merozoite antigen of 250-350 kd molecular weight which is reactive with anti-merozoite monoclonal antibodies secreted by hybridoma clone 1.90 (ATCC No. HB8336); and a pharmaceutically-acceptable carrier therefore; wherein said antigen is soluble in a detergent containing buffer.
Claim 2
Dependent Claim
57 words
An antigenic, immunogenic, proteinaceous vaccine for combatting Eimeria spp. infection in avians which comprises: an effective amount of Eimeria tenella merozoite antigen of 250-350 kd molecular weight which is reactive with anti-merozoite monoclonal antibodies secreted by hybridoma clone 1.90 (ATCC No. HB8336); and a pharmaceutically-acceptable carrier therefore; wherein said antigen is soluble in a detergent containing buffer.
Claim 3
Dependent Claim
15 words
A vaccine according to claim 1 additionally containing a stabilizer or pharmaceutically acceptable adjuvant therefore.
Claim 4
Dependent Claim
62 words
An antigenic, immunogenic, proteinaceous vaccine for combatting Eimeria spp. infection in avians which comprises: an effective amount of Eimeria tenella merozoite antigen of 110-150 kd molecular weight which is reactive with anti-merozoite monoclonal antibodies secreted by hybridoma clone 10.84 (ATCC No. HB8387) and 15.84 (ATCC No. HB8335), and a pharmaceutically-acceptable carrier therefore; wherein said antigen is soluble in a detergent containing buffer.
Claim 5
Dependent Claim
62 words
An antigenic, immunogenic, proteinaceous vaccine for combatting Eimeria spp. infection in avians which comprises: an effective amount of Eimeria tenella merozoite antigen of 110-150 kd molecular weight which is reactive with anti-merozoite monoclonal antibodies secreted by hybridoma clone 10.84 (ATCC No. HB8387) and 15.84 (ATCC No. HB8335), and a pharmaceutically-acceptable carrier therefore; wherein said antigen is soluble in a detergent containing buffer.
Claim 6
Dependent Claim
15 words
A vaccine accoring to claim 3 additionally containing a stabilizer or pharmaceutically acceptable adjuvant therefore.
Claim 7
Dependent Claim
66 words
An antigenic, immunogenic, proteinaceous vaccine for combatting Eimeria spp. infection in avians which comprises: an effective amount of Eimeria tenella merozoite antigen of 15-21 kd molecular weight which is reactive with anti-merozoite monoclonal antibodies secreted by hybridoma clones 8.03 (ATCC No. HB8388), 2.03 (ATCC No. HB8389) and 13.90 (ATCC No. HB8337); and a pharmaceutically-acceptable carrier therefore; wherein said antigen is soluble in a detergent containing buffer.
Claim 8
Dependent Claim
66 words
An antigenic, immunogenic, proteinaceous vaccine for combatting Eimeria spp. infection in avians which comprises: an effective amount of Eimeria tenella merozoite antigen of 15-21 kd molecular weight which is reactive with anti-merozoite monoclonal antibodies secreted by hybridoma clones 8.03 (ATCC No. HB8388), 2.03 (ATCC No. HB8389) and 13.90 (ATCC No. HB8337); and a pharmaceutically-acceptable carrier therefore; wherein said antigen is soluble in a detergent containing buffer.
Claim 9
Dependent Claim
15 words
A vaccine according to claim 5 additionally containing a stabilizer or pharmaceutically acceptable adjuvant therefore.
Claim 10
Independent Claim
62 words
A method of combatting Eimeria spp. infection in avians, said method comprising: administering intraperitoneally, orally or intramuscularly into domestic species, one or a combination of antigens reactive with anti-merozoite monoclonal antibodies secreted by hybridoma clone 1.90 (ATCC No. HB8336), 10.84 (ATCC No. HB8374), 15.84 (ATCC No. HB8335), 8.03 (ATCC No. HB8388), 2.03 (ATCC No. HB8389), 13.90 (ATCC No. HB8337) or mixtures thereof.
Claim 11
Independent Claim
62 words
A method of combatting Eimeria spp. infection in avians, said method comprising: administering intraperitoneally, orally or intramuscularly into domestic species, one or a combination of antigens reactive with anti-merozoite monoclonal antibodies secreted by hybridoma clone 1.90 (ATCC No. HB8336), 10.84 (ATCC No. HB8374), 15.84 (ATCC No. HB8335), 8.03 (ATCC No. HB8388), 2.03 (ATCC No. HB8389), 13.90 (ATCC No. HB8337) or mixtures thereof.
Claim 12
Dependent Claim
15 words
A vaccine according to claim 1 additionally containing a stabilizer and pharmaceutically acceptable adjuvant therefore.
Claim 13
Dependent Claim
15 words
A vaccine according to claim 3 additionally containing a stabilizer and pharmaceutically acceptable adjuvant therefore.
Claim 14
Dependent Claim
15 words
A vaccine according to claim 5 additionally containing a stabilizer and pharmaceutically acceptable adjuvant therefore.
References Cited (Backward Citations)
US3147186A
Cited
US4438097A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-03-16 | 1990-09-15 | 1991-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-16 | 1994-09-15 | 1995-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-16 | 1998-09-15 | 1999-03-17 | $7700.00 | Window Closed / Lapsed |