Stable coccidiosis immunization

Claim 1

THE METHOD WHICH COMPRISES THE STEPS OF: INFECTING A HEALTHY, DISEASE-FREE CHICKEN ONE DAY TO TWELVE WEEKS OF AGE BY INOCULATION WITH AN ORAL DOSE OF VIABLE SPORULATED OOCYSTS OF EIMERIA TENELLA IN WHICH THE NUMBER OF SAID VIABLE SPORULATED OOCYSTS IS APPROXIMATELY 100-300 FOR A CHICKEN ONE DAY OLD AND IS INCREASED WITH INCREASING AGE AND WEIGHT OF THE CHICKEN TO A MAXIMUM OF APPROXIMATELY 40,000 FOR A CHICKEN TWELVE WEEKS OLD AND WEIGHING APPROXIMATELY FIVE POUNDS; HARVESTING DURING THE PERIOD FROM 144 HOURS TO 288 HOURS AFTER THE INOCULATION THE OOCYSTS OF EIMERIA TENELLA PRODUCED BY THE INFECTION BY PERIODICALLY COLLECTING THE MOIST FRESH CECAL DROPPINGS FROM THE INFECTED CHICKEN OVER A PERIOD OF UP TO 96 HOURS BEGINNING 144-192 HOURS AFTER THE INOCULATION; MIXING THE HARVESTED OOCYSTS, WITHIN APPROXIMATELY FOUR HOURS AFTER COLLECTION THEREOF, WITH A ONE OF FOUR PERCENT AQUEOUS POTASSIUM DICHROMATE SOLUTION AND REMOVING COARSE INSOLUBLE PARTICLES OF DEBRIS FROM THE SUSPENSIONS OF OOCYSTS THUS OBTAINED; AERATING THE OOCYSTS, BEGINNING WITHIN APPROXIMATELY EIGHTEEN HOURS AFTER THEIR COLLECTION, IN A ONE TO FOUR PERCENT AQUEOUS POTASSIUM DICHROMATE SOLUTION AT A TEMPERATURE IN THE APPROXIMATE RANGE 19-37*C., THEREBY CAUSING SPORULATION OF THE OOCYSTS; WASHING THE SPORULATED OOCYSTS WITH ONE TO FOUR PERCENT AQUEOUS POTASSIUM DICHROMATE SOLUTION; AND RECOVERING THE SPORULATED OOCYSTS OF EIMERIA TENELLA THUS PRODUCED.

Claim 2

THE METHOD WHICH COMPRISES THE STEPS OF: INFECTING A HEALTHY, DISEASE-FREE CHICKEN ONE DAY TO TWELVE WEEKS OF AGE BY INOCULATION WITH AN ORAL DOSE OF VIABLE SPORULATED OOCYSTS OF EIMERIA TENELLA IN WHICH THE NUMBER OF SAID VIABLE SPORULATED OOCYSTS IS APPROXIMATELY 100-300 FOR A CHICKEN ONE DAY OLD AND IS INCREASED WITH INCREASING AGE AND WEIGHT OF THE CHICKEN TO A MAXIMUM OF APPROXIMATELY 40,000 FOR A CHICKEN TWELVE WEEKS OLD AND WEIGHING APPROXIMATELY FIVE POUNDS; HARVESTING DURING THE PERIOD FROM 144 HOURS TO 288 HOURS AFTER THE INOCULATION THE OOCYSTS OF EIMERIA TENELLA PRODUCED BY THE INFECTION BY PERIODICALLY COLLECTING THE MOIST FRESH CECAL DROPPINGS FROM THE INFECTED CHICKEN OVER A PERIOD OF UP TO 96 HOURS BEGINNING 144-192 HOURS AFTER THE INOCULATION; MIXING THE HARVESTED OOCYSTS, WITHIN APPROXIMATELY FOUR HOURS AFTER COLLECTION THEREOF, WITH A ONE OF FOUR PERCENT AQUEOUS POTASSIUM DICHROMATE SOLUTION AND REMOVING COARSE INSOLUBLE PARTICLES OF DEBRIS FROM THE SUSPENSIONS OF OOCYSTS THUS OBTAINED; AERATING THE OOCYSTS, BEGINNING WITHIN APPROXIMATELY EIGHTEEN HOURS AFTER THEIR COLLECTION, IN A ONE TO FOUR PERCENT AQUEOUS POTASSIUM DICHROMATE SOLUTION AT A TEMPERATURE IN THE APPROXIMATE RANGE 19-37*C., THEREBY CAUSING SPORULATION OF THE OOCYSTS; WASHING THE SPORULATED OOCYSTS WITH ONE TO FOUR PERCENT AQUEOUS POTASSIUM DICHROMATE SOLUTION; AND RECOVERING THE SPORULATED OOCYSTS OF EIMERIA TENELLA THUS PRODUCED.