Patent US4643968A successfully refreshed from Google Patents.
US4643968A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for determining metabolism and growth of cells under various conditions
Assignee: Massachusetts Institute of Technology
•
Filing: 1983-08-26
•
Granted: 1987-02-17
•
Expires: 2003-08-26
Abstract
Abstract The metabolism and growth of cells is measured by suspending the cells in a liquid medium capable of forming a gel. The resultant suspension is formed into small droplets which droplets are caused to gel. Samples of the gel microdroplets (GMDs) are treated to alter the metabolism or growth of the cells, and the amount of metabolites, or other cellular products or external reaction products within each GMD is measured. The process can be utilized to determine essentially simultaneously the enumeration or count and the antibiotic susceptibility, or susceptibility to other compounds, factors, biological agents or other cells, of the cells which are representative of a larger sample of said cells.
Inventors
- 👤 James C. Weaver
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 08:06
CPC Classifications
C; C12; C12Q; C12Q1/00; C12N; C12N1/00; C12P; C12P1/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
211 words
A process for determining the effects of at least one composition of matter upon a microbiologically active material selected from the group consisting of a macromolecule, a viroid, a virus, a bacterium, a yeast, a mold, a parasite, a plant cell and a mammalian cell in a sample comprising the steps of: forming a plurality of subsamples from the sample of microbiologically active material comprising at least one test subsample and at least one control subsample, each of said subsamples being made by: (i) forming a suspension of the microbiologically active material in a liquid diluent capable of forming a gel, each said suspension comprising a plurality of liquid micro-droplets, each of said liquid micro-droplets containing not substantially more than one microbiologically active entity: (ii) converting said liquid micro-droplets of each said suspension into gel micro-droplets having a diameter between about 0.2 and 1000 microns; combining said test subsamples with a composition of interest while maintaining said control subsamples as uncombined suspensions of micro-droplets; measuring a characteristic product of microbiological activity for each of said combined subsamples and each of said uncombined subsamples; and comparing said measured characteristic product from said combined subsamples to said measured characteristic product from said uncombined subsamples to determine the effect of the composition of interest.
Claim 2
Dependent Claim
211 words
A process for determining the effects of at least one composition of matter upon a microbiologically active material selected from the group consisting of a macromolecule, a viroid, a virus, a bacterium, a yeast, a mold, a parasite, a plant cell and a mammalian cell in a sample comprising the steps of: forming a plurality of subsamples from the sample of microbiologically active material comprising at least one test subsample and at least one control subsample, each of said subsamples being made by: (i) forming a suspension of the microbiologically active material in a liquid diluent capable of forming a gel, each said suspension comprising a plurality of liquid micro-droplets, each of said liquid micro-droplets containing not substantially more than one microbiologically active entity: (ii) converting said liquid micro-droplets of each said suspension into gel micro-droplets having a diameter between about 0.2 and 1000 microns; combining said test subsamples with a composition of interest while maintaining said control subsamples as uncombined suspensions of micro-droplets; measuring a characteristic product of microbiological activity for each of said combined subsamples and each of said uncombined subsamples; and comparing said measured characteristic product from said combined subsamples to said measured characteristic product from said uncombined subsamples to determine the effect of the composition of interest.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein said subsamples are formed prior to forming said gel micro-droplets.
Claim 4
Dependent Claim
16 words
The process of claim 1 wherein said subsamples are formed subsequent to forming said gel micro-droplets.
Claim 5
Dependent Claim
21 words
The process of claim 1 wherein said subsamples are combined with said composition of interest prior to forming said gel microdroplets.
Claim 6
Dependent Claim
21 words
The process of claim 1 wherein said subsamples are combined with said composition of interest subsequent to forming said gel micro-droplets.
Claim 7
Dependent Claim
31 words
The process of claim 1 wherein the microbiologically active material comprises at least one bacteria, and the composition of interest combined with said microbiologically active material comprises at least one antibiotic.
Claim 8
Dependent Claim
19 words
The process of claim 1 wherein said control subsamples comprise more than one medium for supporting metabolism and growth.
Claim 9
Dependent Claim
17 words
The process of claim 7 wherein the microbiologically active material is at least one type of bacteria.
Claim 10
Dependent Claim
33 words
The process of claim 7 wherein the microbiologically active material is at least one type of bacteria, and the composition of interest combined with said microbiologically active material is at least one antibiotic.
Claim 11
Dependent Claim
41 words
The process of any one of claims 1, 2, 3, 4, 5 and 7 wherein the composition of interest combined with said microbiologically active material comprises at least one cell which secretes a composition which biologically affects said microbiologically active material.
References Cited (Backward Citations)
US4061466A
Cited
JPS5799195A
Cited
US4242447A
Cited
US4391909A
Cited
US4401755A
Cited
EP0075215A1
Cited
WO1983003102A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-16 | 1990-08-18 | 1991-02-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-16 | 1994-08-18 | 1995-02-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-16 | 1998-08-18 | 1999-02-17 | $7700.00 | Window Closed / Lapsed |