US4642411A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Tryptophan overproducer mutants of cereal crops
Assignee: DeKalb Genetics Corp
•
Filing: 1985-02-21
•
Granted: 1987-02-10
•
Expires: 2005-02-21
Abstract
Abstract This invention is direction to the production of novel plant genotypes or lines which produce increased free tryptophan levels and are resistant to inhibition by analogs which normally inhibit the activity of the enzyme anthranilate synthase before alteration. This invention further relates to genes encoding such exzymes, and to processes for utilizing these novel genes and enzymes. Further products of the invention include plants, plant tissues and seeds which exhibit resistance to such analogs and overproduce tryptophan resulting from expression of genes encoding analog resistant anthranilate synthase enzyme.
Inventors
- 👤 Kenneth A. Hibberd
- 👤 Paul C. Anderson
- 👤 Melanie Barker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 15:58
CPC Classifications
A; A01; A01H; A01H5/00; A01H5/10; A01H1/00; A01H6/00; A01H6/46; C; C12; C12N; C12N15/00; C12N15/01; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/8251; C12N15/8254; C12N5/00; C12N5/04; C12N9/00; C12N9/88; Y; Y10; Y10S; Y10S47/00; Y10S47/01
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
47 words
A monocotyledonous seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 2
Dependent Claim
47 words
A monocotyledonous seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 3
Dependent Claim
48 words
A monocotyledonous seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 4
Dependent Claim
48 words
A monocotyledonous seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 5
Dependent Claim
49 words
A hybrid mnocotyledonous seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 6
Dependent Claim
49 words
A hybrid mnocotyledonous seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 7
Dependent Claim
49 words
A hybrid monocotyledonous seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 8
Dependent Claim
49 words
A hybrid monocotyledonous seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 9
Dependent Claim
18 words
The monocotyledonous seed according to claim 1, 2, 3, or 4, wherein the monocotyledon is a cereal crop.
Claim 10
Dependent Claim
20 words
The monocotyledonous seed according to claim 5, wherein the cereal crop is rice, wheat, barley, sorghum, oats, rye or millet.
Claim 11
Dependent Claim
55 words
A monocotyledonous plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 12
Dependent Claim
55 words
A monocotyledonous plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 13
Dependent Claim
54 words
A monocotyledonous plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least one milligram per gram dry seed weight.
Claim 14
Dependent Claim
54 words
A monocotyledonous plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least one milligram per gram dry seed weight.
Claim 15
Dependent Claim
56 words
A hybrid monocotyledonous plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 16
Dependent Claim
56 words
A hybrid monocotyledonous plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 17
Dependent Claim
56 words
A hybrid monocotyledonous plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 18
Dependent Claim
56 words
A hybrid monocotyledonous plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 19
Dependent Claim
18 words
The monocotyledonous plant according to claim 7, 8, 9, or 10, wherein the monocotyledon is a cereal crop.
Claim 20
Dependent Claim
20 words
The monocotyledonous plant according to claim 11, wherein the cereal crop is rice, wheat, barley, sorghum, oats, rye or millet.
Claim 21
Dependent Claim
61 words
A monocotyledonous tissue culture capable of generating a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 22
Dependent Claim
61 words
A monocotyledonous tissue culture capable of generating a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 23
Dependent Claim
59 words
A monocotyledonous tissue culture capable of generating a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram dry seed weight.
Claim 24
Dependent Claim
59 words
A monocotyledonous tissue culture capable of generating a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram dry seed weight.
Claim 25
Dependent Claim
17 words
The monocotyledonous tissue culture according to claim 13 or 14, wherein the monocotyledon is a cereal crop.
Claim 26
Dependent Claim
21 words
The monocotyledonous tissue culture according to claim 15, wherein the cereal crop is rice, wheat, barley, sorghum, oats, rye or millet.
Claim 27
Independent Claim
135 words
A method for producing a tryptophan overproducing monocotyledonous plant, comprising the steps of: (a) isolating an appropriate donor tissue from a monocotyledonous plant and growing said tissue in an initiation/maintenance medium to initiate and maintain the growth of plant tissue cultures; (b) growing said plant tissue cultures in the presence of a tryptophan analog at a level which inhibits from about 60% to about 80% of growth during the first four weeks; (c) repeatedly subculturing the plant tissue cultures of step (b) in the presence of the tryptophan analog; (d) identifying a stable analog resistant plant cell line from step (c) which grows at a rate comparable to that of a plant tissue culture grown in the absence of a tryptophan analog; and (e) regenerating a plant from said stable analog resistant plant cell line.
Claim 28
Independent Claim
135 words
A method for producing a tryptophan overproducing monocotyledonous plant, comprising the steps of: (a) isolating an appropriate donor tissue from a monocotyledonous plant and growing said tissue in an initiation/maintenance medium to initiate and maintain the growth of plant tissue cultures; (b) growing said plant tissue cultures in the presence of a tryptophan analog at a level which inhibits from about 60% to about 80% of growth during the first four weeks; (c) repeatedly subculturing the plant tissue cultures of step (b) in the presence of the tryptophan analog; (d) identifying a stable analog resistant plant cell line from step (c) which grows at a rate comparable to that of a plant tissue culture grown in the absence of a tryptophan analog; and (e) regenerating a plant from said stable analog resistant plant cell line.
Claim 29
Dependent Claim
13 words
The method according to claim 17, wherein the monocotyledon is a cereal crop.
Claim 30
Dependent Claim
19 words
The method according to claim 18, wherein the cereal crop is rice, wheat, barley, sorghum, oats, rye or millet.
Claim 31
Independent Claim
36 words
A method for producing hybrid monocotyledonous seed, comprising crossing a first line capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, and another line.
Claim 32
Independent Claim
36 words
A method for producing hybrid monocotyledonous seed, comprising crossing a first line capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, and another line.
Claim 33
Independent Claim
36 words
A method for producing hybrid monocotyledonous seed, comprising crossing a first line capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, and another line.
Claim 34
Independent Claim
36 words
A method for producing hybrid monocotyledonous seed, comprising crossing a first line capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, and another line.
Claim 35
Dependent Claim
15 words
The method according to claim 20 or 21, wherein the monocotyledon is a cereal crop.
Claim 36
Dependent Claim
19 words
The method according to claim 22, wherein the cereal crop is rice, wheat, barley, sorghum, oats, rye or millet.
References Cited (Backward Citations)
US4443971A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-09 | 1990-08-11 | 1991-02-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-09 | 1994-08-11 | 1995-02-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-09 | 1998-08-11 | 1999-02-10 | $7700.00 | Window Closed / Lapsed |