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US4642334A
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Hybrid DNA prepared binding composition
Assignee: Merck Sharp & Dohme LLC
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Filing: 1983-12-05
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Granted: 1987-02-10
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Expires: 2003-12-05
Abstract
Abstract Proteinaceous binding compositions are prepared employing hybrid DNA technology, where the variable region polypeptides of immunoglobulins are substantially reproduced to provide relatively small protein molecules having binding specificity and lacking the undesirable aspects of the heavy regions of immunoglobulins. The compositions find a wide range of use, particularly for physiological purposes for diagnosis and therapy. The binding compositions may be modified by labeling with radioisotopes, fluorescers, and toxins for specific applications in diagnosis or therapy.
Inventors
- 👤 Kevin W. Moore
- 👤 Alejandro Zaffaroni
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 13:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; A; A61; A61P; A61P37/00; C; C07; C07K; C07K16/00; C12; C12N; C12N15/00; C12N15/09; C12N15/10; A61K; A61K38/00; C07K2317/00; C07K2317/60; C07K2317/62; C07K2317/622; Y; Y10; Y10S; Y10S424/00; Y10S424/801; Y10S530/00; Y10S530/808; Y10S530/809; Y10S530/866; Y10S530/867; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
100 words
A specific binding composition ("rFv") comprising two polypeptide chains having substantially the same amino acid sequence of at least a portion of the variable region, without constant region amino acids, of a mammalian immunoglobulin, said immunoglobulin having binding specificity to a predetermined ligand, wherein said polypeptide chains are prepared by expression of a DNA sequence coding for the variable region, said expression occurring in the absence of expression of a DNA sequence coding for natively associated constant region, and wherein said two polypeptide chains combine to form the rFv which has a high affinity and specificity for said predetermined ligand.
Claim 2
Dependent Claim
100 words
A specific binding composition ("rFv") comprising two polypeptide chains having substantially the same amino acid sequence of at least a portion of the variable region, without constant region amino acids, of a mammalian immunoglobulin, said immunoglobulin having binding specificity to a predetermined ligand, wherein said polypeptide chains are prepared by expression of a DNA sequence coding for the variable region, said expression occurring in the absence of expression of a DNA sequence coding for natively associated constant region, and wherein said two polypeptide chains combine to form the rFv which has a high affinity and specificity for said predetermined ligand.
Claim 3
Dependent Claim
43 words
A composition according to claim 1, wherein said two polypeptide chains are the light chain of from about 95 to 115 amino acids and the heavy chain of from about 110 to 125 amino acids, wherein said heavy chain includes the D region.
Claim 4
Dependent Claim
37 words
A composition according to any one of claims 1 or 2, wherein each of said chains includes at least two cysteines separated by from about 60 to 70 amino acids and joined together through a disulfide link.
Claim 5
Dependent Claim
25 words
A composition according to any one of claims 1 or 2 wherein said rFv is labeled with a functionality capable of producing a detectable signal.
Claim 6
Dependent Claim
20 words
A composition according to any one of claims 1 or 2 wherein said rFv is labeled with a cytotoxic agent.
Claim 7
Dependent Claim
13 words
A composition according to claim 5, wherein said cytotoxic agent is a radionuclide.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-09 | 1990-08-11 | 1991-02-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-09 | 1994-08-11 | 1995-02-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-09 | 1998-08-11 | 1999-02-10 | $7700.00 | Window Closed / Lapsed |