Patent US1974884A successfully refreshed from Google Patents.
Patent US4622291A successfully refreshed from Google Patents.
US4622291A
Expired / Lapsed
US
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Method and device for quantitative end point determination in immunofluorescence using microfluorophotometry
Assignee: United States Department of Health and Human Services
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Filing: 1984-06-11
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Granted: 1986-11-11
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Expires: 2004-06-11
Abstract
Abstract The present invention discloses a device and a process for quantitation of end point in the formation of fluorescent reaction product in microfluorophotometry. The process comprises: (a) incorporating a protective agent in a suitable mounting medium in an amount sufficient to reduce fading of fluorescent reaction product less than 25% of initial fluorescent intensity; (b) calibrating photometer used in said microscopy with a stable emitter; and (c) recording the intensity of fluorescence of said fluorescent reaction product by means for measuring light intensity. The invention also includes a device for calibration of the photometer and a kit comprising separate containers for suitable mounting medium, buffer, suitable immunofluorescent reagents, fading retardant means, a photometer calibrating device and the like and optional instructions.
Inventors
- 👤 Grace L. Picciolo
- 👤 David S. Kaplan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 22:37
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N21/6456; G01N21/6458; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; G01N21/6428; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; G01N33/543; G01N33/54313; G01N2201/00; G01N2201/12; G01N2201/122; G01N2201/1222; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/807; Y10S436/826; Y10T; Y10T436/00; Y10T436/11; Y10T436/112499
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
76 words
A method for quantitative determination of fluorescent endpoint of a fluorescent reaction in photometric microscopy comprising: (a) incorporating a protective agent in a mounting medium for fluorescent reaction in an amount sufficient to reduce fading of fluorescent reaction product less than 25% of initial fluorescent intensity; (b) calibrating the photometer used in said microscopy with a stable emitter; and (c) recording the intensity of fluorescence of said fluorescent reaction product by means for measuring light intensity.
Claim 2
Independent Claim
76 words
A method for quantitative determination of fluorescent endpoint of a fluorescent reaction in photometric microscopy comprising: (a) incorporating a protective agent in a mounting medium for fluorescent reaction in an amount sufficient to reduce fading of fluorescent reaction product less than 25% of initial fluorescent intensity; (b) calibrating the photometer used in said microscopy with a stable emitter; and (c) recording the intensity of fluorescence of said fluorescent reaction product by means for measuring light intensity.
Claim 3
Dependent Claim
22 words
The method of claim 1, wherein said protective agent is selected from the group consisting of sodium dithionite, dithioerythritol, dithiothreitol and triethylenediamine.
Claim 4
Dependent Claim
11 words
The method of claim 2 wherein said protective agent is dithioerythritol.
Claim 5
Dependent Claim
26 words
The method of claim 2 wherein the amount of said protective agent in said mounting medium is in the range of about 0.01M to about 0.3M.
Claim 6
Dependent Claim
25 words
The method of claim 4 wherein said mounting medium comprises a suitable buffered medium having a pH value ranging from about 6.6 to about 9.4.
Claim 7
Dependent Claim
16 words
The method of claim 5 wherein said pH value range from about 7.5 to about 8.4.
Claim 8
Dependent Claim
16 words
The method of claim 6 wherein said buffered medium comprises about 0.05M glycine or trizma base.
Claim 9
Dependent Claim
14 words
The method of claim 7 wherein said buffered medium comprises about 0.05M trizma base.
Claim 10
Dependent Claim
30 words
The method of claim 1 wherein said stable emitter is selected from the group consisting of organic and inorganic particles coated or impregnated with any material that are fluorescence emitters.
Claim 11
Dependent Claim
26 words
The method of claim 9 wherein said emitter is uranium impregnated glass, fluorescently labelled polystyrene spheres, fluorescently labelled latex spheres, inorganic ions and inorganic phosphor particles.
Claim 12
Dependent Claim
12 words
The method of claim 10 wherein said emitter is uranyl glass plate.
Claim 13
Dependent Claim
20 words
The method of claim 1 wherein said means for measuring light intensity is a computer controlled microscope voltage output photometer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-11-10 | 1990-05-12 | 1990-11-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-11-10 | 1994-05-12 | 1994-11-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-11-10 | 1998-05-12 | 1998-11-11 | $7700.00 | Window Closed / Lapsed |