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US4620930A Expired / Lapsed US ⏱️ 0.0 years remaining

Process for rapidly determining biological toxicity of wastewater

Assignee: Polybac Corp
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Filing: 1985-12-16
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Granted: 1986-11-04
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Expires: 2005-12-16
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Abstract

Abstract This invention pertains to a method for analyzing the biochemical decomposibility of aqueous substrates by microorganisms and particularly to a method for analyzing the quality or biological toxicity of incoming sewage to a sewage disposal plant. The process comprises: placing a preselected amount of bacterial culture, in the form of a dried powder, in contact with a preselected amount of aqueous waste containing greater than 5 mg/l dissolved oxygen, the bacterial culture being capable of effecting aerobic biodegradation of organic waste; measuring the dissolved oxygen content of the aqueous waste during rehydration and respiration development of the microorganisms as a function of time; determining the rate of dissolved oxygen decline at a preselected time; and then comparing the rate of dissolved oxygen decline to the rate of dissolved oxygen for a preselected baseline standard substrate. By comparing the ratio of dissolved oxygen decline at a preselected time with respect to a test sample and a standard substrate one can calculate the relative toxicity or inhibition of the incoming sewage to aerobic respiration and growth.

Inventors

  • 👤 Curtis S. McDowell

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-08 21:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/18; G01N33/1806; C; C02; C02F; C02F3/00; C02F3/006; Y; Y02; Y02W; Y02W10/00; Y02W10/10; Y10; Y10T; Y10T436/00; Y10T436/20; Y10T436/207497; Y10T436/209163

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 184 words
In a process for determining the quality of a wastewater solution or a solution of a specific chemical which comprises mixing microorganisms with a test solution, measuring the dissolved oxygen content in the test solution, and correlating dissolved oxygen content of the mixture with a standard solution, the improvement for enhancing the sensitivity of the test while reducing the amount of time necessary to conduct a test which comprises: a. utilizing a bacterial culture in the form of a dried powder, the bacterial culture being capable of aerobic biodegradation of organic wastes; b. placing a preselected amount of said bacterial culture into a test vessel containing a preselected amount of aqueous waste which has been saturated with oxygen; c. measuring the dissolved oxygen content in the test sample as a function of time; d. determining the rate of dissolved oxygen decline at a preselected time, and, e. determining the ratio between the rate of dissolved oxygen decline at said preselected time and the rate of decline for a preselected baseline standard solution, and thereby determining the degree of inhibition of bacterial respiration or growth.
Claim 2
Dependent Claim 184 words
In a process for determining the quality of a wastewater solution or a solution of a specific chemical which comprises mixing microorganisms with a test solution, measuring the dissolved oxygen content in the test solution, and correlating dissolved oxygen content of the mixture with a standard solution, the improvement for enhancing the sensitivity of the test while reducing the amount of time necessary to conduct a test which comprises: a. utilizing a bacterial culture in the form of a dried powder, the bacterial culture being capable of aerobic biodegradation of organic wastes; b. placing a preselected amount of said bacterial culture into a test vessel containing a preselected amount of aqueous waste which has been saturated with oxygen; c. measuring the dissolved oxygen content in the test sample as a function of time; d. determining the rate of dissolved oxygen decline at a preselected time, and, e. determining the ratio between the rate of dissolved oxygen decline at said preselected time and the rate of decline for a preselected baseline standard solution, and thereby determining the degree of inhibition of bacterial respiration or growth.
Claim 3
Dependent Claim 23 words
The process of claim 1 wherein the bacterial culture contains an organism or organisms of pseudomonad, bacillus, aerobacter, azotobacter, arthrobacter, micrococcus, and nocardia.
Claim 4
Dependent Claim 37 words
The process of claim 2 wherein said dissolved oxygen content is measured at a preselected time within a range of 10 to 30 minutes or before the residual dissolved oxygen drops below 0.5 mg/l whichever is first.
Claim 5
Dependent Claim 37 words
The process of claim 3 wherein said microorganisms are combined with sufficient buffers to maintain a desired pH range during the procedure and said process is used to determine the quality of sewage containing phenol or formaldehyde.
Claim 6
Dependent Claim 24 words
The process of claim 1 wherein the quantity of preselected cultures employed is from 5 to 15 grams per 500 ml of sample solution.
Claim 7
Dependent Claim 16 words
The process of claim 5 wherein the bacterial culture employed is a mutant bacteria containing pseudomonads.

References Cited (Backward Citations)

US3510407A Cited
US4162195A Cited
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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1989-11-03 1990-05-05 1990-11-04 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1993-11-03 1994-05-05 1994-11-04 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1997-11-03 1998-05-05 1998-11-04 $7700.00 Window Closed / Lapsed

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