US4612288A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Oxirane resins for enzyme immobilization
Assignee: Rohm and Haas Co
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Filing: 1986-01-13
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Granted: 1986-09-16
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Expires: 2006-01-13
Abstract
Abstract Carriers for immobilizing enzymes may be prepared by suspension copolymerizing an oxirane-group-bearing, monovinyl monomer and a major amount of a trivinyl crosslinking monomer having a hydrophilic character, in the presence of a phase separator which does not react with the oxirane group. The resulting carriers, in bead form, have high porosity, high surface area, and pores of diameter sufficient for ready penetration by enzymes and substrates.
Inventors
- 👤 Michael P. Bigwood
- 👤 John O. Naples
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 23:50
CPC Classifications
C; C08; C08F; C08F216/00; C08F216/12; C08F216/14; C08F220/00; C08F220/02; C08F220/10; C08F220/26; C08F220/32; C08F220/325; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
128 words
An oxirane-group-bearing porous carrier bead for immobilized enzymes upon which an enzyme has been immobolized by chemically attaching the hydrogen bearing group or the proteinaceous portion of the enzyme to the porous carrier at the site of the oxirane group, the porous carrier being prepared by free-radical, suspension polymerizing an oxirane-group-bearing monovinyl monomer selected from the group consisting of glycidyl acrylate, glycidyl methacrylate, allyl glycidyl ether, and mixtures thereof, and a trivinyl crosslinking monomer having hydrophilic character, the ratio of monovinyl monomer to trivinyl monomer being from about 5:95 to about 50:50 by weight, in the presence of about 20% to about 90%, based on the weight of the organic phase, of a phase separator which does not react with the oxirane group, in an aqueous suspension medium.
Claim 2
Dependent Claim
128 words
An oxirane-group-bearing porous carrier bead for immobilized enzymes upon which an enzyme has been immobolized by chemically attaching the hydrogen bearing group or the proteinaceous portion of the enzyme to the porous carrier at the site of the oxirane group, the porous carrier being prepared by free-radical, suspension polymerizing an oxirane-group-bearing monovinyl monomer selected from the group consisting of glycidyl acrylate, glycidyl methacrylate, allyl glycidyl ether, and mixtures thereof, and a trivinyl crosslinking monomer having hydrophilic character, the ratio of monovinyl monomer to trivinyl monomer being from about 5:95 to about 50:50 by weight, in the presence of about 20% to about 90%, based on the weight of the organic phase, of a phase separator which does not react with the oxirane group, in an aqueous suspension medium.
Claim 3
Dependent Claim
33 words
The carrier bead of claim 1 wherein the enzyme is selected from the group consisting of penicillin G acylase, penicillin V acylase, glucose isomerase, glucoamylase, lactase, thermoase, cyanide hydrolase, cephalosporin hydrolase and esterase.
References Cited (Backward Citations)
US4070348A
Cited
US4246351A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-09-15 | 1990-03-17 | 1990-09-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-09-15 | 1994-03-17 | 1994-09-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-09-15 | 1998-03-17 | 1998-09-16 | $7700.00 | Window Closed / Lapsed |