US4246351A
Expired / Lapsed
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Protein adsorbent
Assignee: Asahi Kasei Corp
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Filing: 1979-10-29
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Granted: 1981-01-20
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Expires: 1999-10-29
Abstract
Abstract A novel protein adsorbent consisting essentially of a porous copolymer obtained by copolymerizing a monomer mixture comprising at least one cyano group-containing monomer and at least one cross-linkable monomer, said copolymer having an average pore diameter (d) of from 40 A to 9,000 A and a total pore volume of from 0.05√X ml to 1.5√X ml per gram of the copolymer in a dry state, in which X designates the weight proportion of said cross-linkable monomer expressed in terms of the percent by weight based on the total monomers. The present porous copolymer adsorbent has a remarkably high protein adsorbing capacity. The adorbent can advantageously be used for various purposes such as purification and separation of proteins, and removal of proteins. Copolymer-protein composites obtained by adsorption of proteins on the present porous copolymer also are industrially useful and have a wide variety of applications.
Inventors
- 👤 Tetsuya Miyake
- 👤 Kunihiko Takeda
- 👤 Akihiko Ikeda
- 👤 Masayuki Mizuno
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 11:41
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/22; C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C08; C08J; C08J9/00; C08J9/28; C08J9/286; C12; C12H; C12H1/00; C12H1/02; C12H1/04; C12H1/0416; C12H1/0424; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S210/00; Y10S210/902; Y10S210/903; Y10S210/905; Y10S435/00; Y10S435/814; Y10S435/815; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
228 words
A method for adsorbing a protein from an aqueous solution or dispersion of the protein which comprises contacting an aqueous solution or a dispersion of a protein with a porous copolymer obtained by copolymerizing a monomer mixture comprising 2 to 98% by weight of at least one cyano group-containing monomer represented by the following general formula (A): ##STR6## wherein R stands for a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, a halogen atom, a cyano group, a methoxy group, an acetoxyl group, or an unsubstituted or substituted phenyl group represented by the formula ##STR7## wherein R' stands for a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, a halogen atom, a cyano group, a methoxy group or an acetoxyl group), and 2 to 98% by weight of at least one cross-linkable monomer having a plurality of CH 2 ═C< groups, said copolymer having an average pore diameter (d) of from 40 A to 9,000 A and a total pore volume of from 0.05 √X ml to 1.5 √X ml per gram of the copolymer in a dry state, in which X designates the weight proportion of said cross-linkable monomer expressed in terms of the percent by weight based on the total monomers, and being employed in an amount of 0.02 to 300 g per liter of said solution or said dispersion.
Claim 2
Independent Claim
228 words
A method for adsorbing a protein from an aqueous solution or dispersion of the protein which comprises contacting an aqueous solution or a dispersion of a protein with a porous copolymer obtained by copolymerizing a monomer mixture comprising 2 to 98% by weight of at least one cyano group-containing monomer represented by the following general formula (A): ##STR6## wherein R stands for a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, a halogen atom, a cyano group, a methoxy group, an acetoxyl group, or an unsubstituted or substituted phenyl group represented by the formula ##STR7## wherein R' stands for a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, a halogen atom, a cyano group, a methoxy group or an acetoxyl group), and 2 to 98% by weight of at least one cross-linkable monomer having a plurality of CH 2 ═C< groups, said copolymer having an average pore diameter (d) of from 40 A to 9,000 A and a total pore volume of from 0.05 √X ml to 1.5 √X ml per gram of the copolymer in a dry state, in which X designates the weight proportion of said cross-linkable monomer expressed in terms of the percent by weight based on the total monomers, and being employed in an amount of 0.02 to 300 g per liter of said solution or said dispersion.
Claim 3
Dependent Claim
24 words
A method as set forth in claim 1 wherein the contacting of said solution or said dispersion with said porous copolymer is batchwise effected.
Claim 4
Dependent Claim
24 words
A method as set forth in claim 1 wherein the contacting of said solution or said dispersion with said porous copolymer is continuously effected.
Claim 5
Dependent Claim
36 words
A method as set forth in claim 1 wherein the contacting of said solution or said dispersion with said porous copolymer is effected at a temperature in the range of from 2° C. to 95° C.
Claim 6
Dependent Claim
23 words
A method as set forth in claim 2 wherein the contacting time is in the range of from 10 seconds to 500 hours.
Claim 7
Dependent Claim
33 words
A method as set forth in claim 3 wherein said solution or said dispersion is supplied at a space velocity in the range of from 0.01 hr -1 to 1,000 hr -1 .
Claim 8
Dependent Claim
22 words
A method as set forth in claim 1 wherein said solution or said dispersion has a pH of from 1 to 11.
Claim 9
Dependent Claim
205 words
A composite comprising a porous copolymer and a protein in an amount sufficient to exert activity of said protein adsorbed thereon, said porous copolymer being a product obtained by copolymerizing a monomer mixture comprising 2 to 98% by weight of at least one cyano group-containing monomer represented by the following general formula (A): ##STR8## wherein R stands for a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, a halogen atom, a cyano group, a methoxy group, an acetoxyl group, or an unsubstituted or substituted phenyl group represented by the formula ##STR9## (wherein R' stands for a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, a halogen atom, a cyano group, a methoxy group or an acetoxyl group), and 2 to 98% by weight of at least one cross-linkable monomer having a plurality of CH 2 ═C< groups, said copolymer having an average pore diameter (d) of from 40 A to 9,000 A and a total pore volume of from 0.05 √X ml to 1.5 √X ml per gram of the copolymer in a dry state, in which X designates the weight proportion of said crosslinkable monomer expressed in terms of the percent by weight based on the total monomers.
Claim 10
Dependent Claim
205 words
A composite comprising a porous copolymer and a protein in an amount sufficient to exert activity of said protein adsorbed thereon, said porous copolymer being a product obtained by copolymerizing a monomer mixture comprising 2 to 98% by weight of at least one cyano group-containing monomer represented by the following general formula (A): ##STR8## wherein R stands for a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, a halogen atom, a cyano group, a methoxy group, an acetoxyl group, or an unsubstituted or substituted phenyl group represented by the formula ##STR9## (wherein R' stands for a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, a halogen atom, a cyano group, a methoxy group or an acetoxyl group), and 2 to 98% by weight of at least one cross-linkable monomer having a plurality of CH 2 ═C< groups, said copolymer having an average pore diameter (d) of from 40 A to 9,000 A and a total pore volume of from 0.05 √X ml to 1.5 √X ml per gram of the copolymer in a dry state, in which X designates the weight proportion of said crosslinkable monomer expressed in terms of the percent by weight based on the total monomers.
Claim 11
Dependent Claim
28 words
A composite as set forth in claim 8 wherein the amount of the protein is at least 10 mg per gram of the copolymer in a dry state.
Claim 12
Dependent Claim
28 words
A composite as set forth in claim 9 wherein the amount of the protein is at least 20 mg per gram of the copolymer in a dry state.
Claim 13
Dependent Claim
14 words
A composite as set forth in claim 8, wherein the protein is an enzyme.
Claim 14
Dependent Claim
22 words
A method as set forth in claim 1 wherein the content of the cyano group-containing monomer is 4 to 95% by weight.
Claim 15
Dependent Claim
22 words
A method as set forth in claim 1 wherein the content of the cyano group-containing monomer is 6 to 90% by weight.
Claim 16
Dependent Claim
27 words
A method as set forth in claim 1 wherein the cyano group-containing monomer is a member selected from the group consisting of acrylonitrile, methacrylonitrile and mixtures thereof.
Claim 17
Dependent Claim
21 words
A method as set forth in claim 1 wherein the cross-linkable monomer is a monomer having 2 to 4 vinyl groups.
Claim 18
Dependent Claim
14 words
A method as set forth in claim 1 wherein the cross-linkable monomer is divinylbenzene.
Claim 19
Dependent Claim
38 words
A method as set forth in claim 1 wherein the cross-linkable monomer is of the formula ##STR10## wherein R 1 and R 2 each independently is hydrogen or methyl, and n is an integer from 1 to 30.
Claim 20
Dependent Claim
22 words
A method as set forth in claim 1 wherein the weight proportion of the cross-linkable monomer is 8 to 80% by weight.
Claim 21
Dependent Claim
29 words
A method as set forth in claim 1 wherein the average pore diameter (d) of said porous copolymer is in the range of from 50 A to 5,000 A.
Claim 22
Dependent Claim
36 words
A method as set forth in claim 1 wherein the volume of pores of said porous copolymer having a diameter of 0.5 d to 2 d is not more than 60% of the total pore volume.
Claim 23
Dependent Claim
39 words
A method as set forth in claim 1 wherein the total pore volume of said porous copolymer is in the range of from 0.2 √X ml to 1.3 √X ml per gram of the copolymer in a dry state.
Claim 24
Dependent Claim
20 words
A method as set forth in claim 1 wherein the protein has a molecular weight of from 1,000 to 1,000,000.
Claim 25
Dependent Claim
16 words
A method as set forth in claim 1 wherein the protein is albumin of human serum.
Claim 26
Dependent Claim
13 words
A method as set forth in claim 1 wherein the protein is α-chymotrypsin.
Claim 27
Dependent Claim
13 words
A method as set forth in claim 1 wherein the protein is lipase.
Claim 28
Dependent Claim
14 words
A method as set forth in claim 1 wherein the protein is an enzyme.
Claim 29
Dependent Claim
13 words
A method as set forth in claim 1 wherein the protein is trypsin.
Claim 30
Dependent Claim
13 words
A method as set forth in claim 1 wherein the protein is chymotrypsin.
Claim 31
Dependent Claim
14 words
A method as set forth in claim 1 wherein the protein is an antigen.
Claim 32
Dependent Claim
14 words
A method as set forth in claim 1 wherein the protein is an antibody.
Claim 33
Dependent Claim
16 words
A method as set forth in claim 1 wherein said porous copolymer has a spherical shape.
Claim 34
Dependent Claim
34 words
A method as set forth in claim 31 wherein said porous copolymer is a product obtained by suspension polymerization which is carried out in an aqueous solution in the presence of a radical initiator.
Claim 35
Dependent Claim
29 words
A composite produced by the method of claim 1 which comprises the porous copolymer and the protein in an amount sufficient to exert activity of said protein adsorbed thereon.
Claim 36
Dependent Claim
28 words
A composite as set forth in claim 33 wherein the amount of said protein is at least 10 mg per gram of the copolymer in a dry state.
Claim 37
Dependent Claim
28 words
A composite as set forth in claim 34 wherein the amount of said protein is at least 20 mg per gram of the copolymer in a dry state.
Claim 38
Dependent Claim
14 words
A composite as set forth in claim 33 wherein the protein is an enzyme.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-20 | 1984-07-21 | 1985-01-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-20 | 1988-07-21 | 1989-01-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-20 | 1992-07-21 | 1993-01-20 | $7700.00 | Window Closed / Lapsed |