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Patent US4610963A successfully refreshed from Google Patents.
US4610963A
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Novel glutathione oxidase, its production and use
Assignee: Takara Shuzo Co Ltd
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Filing: 1984-12-05
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Granted: 1986-09-09
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Expires: 2004-12-05
Abstract
Abstract A novel glutathione oxidase which acts on reduced glutathione alone and not no other sulfhydryl compounds is obtained by culturing Calodon or Cortinarius of the class Basidiomycetes.
Inventors
- 👤 Susumu Matsui
- 👤 Satoko Uchida
- 👤 Tsutomu Taniguchi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 17:10
CPC Classifications
C; C12; C12Y; C12Y108/00; C12Y108/03; C12Y108/03003; C12N; C12N9/00; C12N9/0004; C12N9/0051; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
168 words
A novel glutathione oxidase T-1 having the following physicochemical properties: (1) Action: Acts on reduced glutathione in the presence of oxygen to produce oxidized glutathione and hydrogen peroxide; (2) Substrate specificity: Acts on reduced glutathione alone, not on other sulfhydryl compounds at all; (3) Optimum pH and pH stability: Has an optimum pH in the vicinity of 7.0, and is stable between pH 4.0 and pH 8.0 for 60-minute treatment at 37° C.; (4) Optimum temperature and thermal stability: Has an optimum temperature in the vicinity of 50° C., and is stable up to 60° C. for 10-minute treatment at a pH of 7.0; (5) Molecular weight: About 450000 by the gel filtration method, and about 55000 by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, from which said novel glutathione oxidase T-1 is composed of eight subunits; (6) Coenzyme: Contains flavine adenine dinucleotide (FAD), a coenzyme, of 4 molecules/molecule; (7) Isoelectric point: 5.65±0.05; (8) Interfering agent: Hg 2+ , p-chloromercuribenzoic acid, sodium L-ascorbate, etc; (9) Sugar content: About 40%.
Claim 2
Dependent Claim
168 words
A novel glutathione oxidase T-1 having the following physicochemical properties: (1) Action: Acts on reduced glutathione in the presence of oxygen to produce oxidized glutathione and hydrogen peroxide; (2) Substrate specificity: Acts on reduced glutathione alone, not on other sulfhydryl compounds at all; (3) Optimum pH and pH stability: Has an optimum pH in the vicinity of 7.0, and is stable between pH 4.0 and pH 8.0 for 60-minute treatment at 37° C.; (4) Optimum temperature and thermal stability: Has an optimum temperature in the vicinity of 50° C., and is stable up to 60° C. for 10-minute treatment at a pH of 7.0; (5) Molecular weight: About 450000 by the gel filtration method, and about 55000 by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, from which said novel glutathione oxidase T-1 is composed of eight subunits; (6) Coenzyme: Contains flavine adenine dinucleotide (FAD), a coenzyme, of 4 molecules/molecule; (7) Isoelectric point: 5.65±0.05; (8) Interfering agent: Hg 2+ , p-chloromercuribenzoic acid, sodium L-ascorbate, etc; (9) Sugar content: About 40%.
Claim 3
Dependent Claim
22 words
A reagent composition for reduced glutathione characterized in that said composition contains the novel glutathione oxidase T-1 as described in claim 1.
Claim 4
Independent Claim
52 words
A method for the quantitative determination of reduced glutathione characterized in that reduced glutathione in test samples is oxidized with said novel glutathione oxidase T-1 in the presence of oxygen, and the reduced glutathione is quantitatively determined based on the amount of consumed oxygen or formed hydrogen peroxide in the reaction solution.
Claim 5
Independent Claim
52 words
A method for the quantitative determination of reduced glutathione characterized in that reduced glutathione in test samples is oxidized with said novel glutathione oxidase T-1 in the presence of oxygen, and the reduced glutathione is quantitatively determined based on the amount of consumed oxygen or formed hydrogen peroxide in the reaction solution.
Claim 6
Independent Claim
42 words
A method for producing said novel glutathione oxidase T-1 characterized in that a novel glutathione oxidase T-1 producing strain belonging to the genus Calodon or Cortinarius is cultured and the novel glutathione oxidase T-1 produced is collected from the cultured broth obtained.
Claim 7
Independent Claim
42 words
A method for producing said novel glutathione oxidase T-1 characterized in that a novel glutathione oxidase T-1 producing strain belonging to the genus Calodon or Cortinarius is cultured and the novel glutathione oxidase T-1 produced is collected from the cultured broth obtained.
Claim 8
Dependent Claim
24 words
A method as described in claim 4, wherein said novel glutathione oxidase T-1 producing strain belonging to the genus Calodon is Calodon suaveolens K-1671.
Claim 9
Dependent Claim
24 words
A method as described in claim 4, wherein said novel glutathione oxidase T-1 producing strain belonging to the genus Cortinarius is Cortinarius bovinus K-946.
Claim 10
Dependent Claim
43 words
A novel glutathione oxidase T-1 according to claim 1 in which it has such physiochemical properties as in (1) that it acts on reduced glutathione in the presence of oxygen but not on L-cysteine, D-cysteine, N-acetyl-L-cysteine, L-cysteine methylester, cysteamine, thiophenol, 2-mercaptoethanol and dithiothreitol.
References Cited (Backward Citations)
JPS57132879A
Cited
JPS57146598A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-09-08 | 1990-03-10 | 1990-09-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-09-08 | 1994-03-10 | 1994-09-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-09-08 | 1998-03-10 | 1998-09-09 | $7700.00 | Window Closed / Lapsed |