US4608336A
Expired / Lapsed
US
⏱️ 0.0 years remaining
#3B theophylline immunoassay employing 9-theophylline reagents
Assignee: Bayer Corp
•
Filing: 1983-05-11
•
Granted: 1986-08-26
•
Expires: 2003-05-11
Abstract
Abstract Immunoassay method and reagent means for determining theophylline in biological fluids such as serum. The antibody employed in the method is prepared from an immunogen comprising theophylline coupled at its 9-position to an immunogenic carrier material.
Inventors
- 👤 Jeffrey L. Benovic
- 👤 Robert T. Buckler
- 👤 John F. Burd
- 👤 Thomas M. Li
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 05:44
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07D; C07D473/00; C07D473/02; C07D473/04; C07D473/06; C07D473/08; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; A; A61; A61K; A61K39/00; A61K2039/60; A61K2039/6031; A61K2039/6087; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/961; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/81; Y10S436/815; Y10S436/822; Y10S436/823
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
80 words
In an immunoassay method for determining theophylline in a test sample, wherein the test sample is combined with an antibody to theophylline and with a labeled form of theophylline or a binding analog of theophylline, and wherein the relative amount of the labeled reagent that becomes bound to the antibody is determined, the improvement which comprises employing as the antibody to theophylline an antibody prepared against an immunogen comprising theophylline covalently linked at its 9-position to an immunogenic carrier material.
Claim 2
Dependent Claim
80 words
In an immunoassay method for determining theophylline in a test sample, wherein the test sample is combined with an antibody to theophylline and with a labeled form of theophylline or a binding analog of theophylline, and wherein the relative amount of the labeled reagent that becomes bound to the antibody is determined, the improvement which comprises employing as the antibody to theophylline an antibody prepared against an immunogen comprising theophylline covalently linked at its 9-position to an immunogenic carrier material.
Claim 3
Dependent Claim
43 words
The method of claim 1 wherein said immunogen is of the formula: ##STR20## wherein R is a chain comprising between 1 and 20 atoms excluding hydrogen, Carrier is said immunogenic carrier material, and p is on the average from 1 to about 50.
Claim 4
Dependent Claim
14 words
The method of claim 2 wherein said carrier material is a protein or polypeptide.
Claim 5
Dependent Claim
21 words
The method of claim 1 wherein the labeled reagent has a detectable property which is altered when bound with said antibody.
Claim 6
Dependent Claim
53 words
In reagent means for determining theophylline by immunoassay comprising an antibody to theophylline and a labeled form of theophylline or a binding analog of theophylline, the improvement which comprises employing as the antibody to theophylline an antibody prepared against an immunogen comprising theophylline covalently linked at its 9-position to an immunogenic carrier material.
Claim 7
Dependent Claim
53 words
In reagent means for determining theophylline by immunoassay comprising an antibody to theophylline and a labeled form of theophylline or a binding analog of theophylline, the improvement which comprises employing as the antibody to theophylline an antibody prepared against an immunogen comprising theophylline covalently linked at its 9-position to an immunogenic carrier material.
Claim 8
Dependent Claim
44 words
The reagent means of claim 5 wherein said immunogen is of the formula: ##STR21## wherein R is a chain comprising between 1 and 20 atoms, excluding hydrogen, Carrier is said immunogenic carrier material, and p is on the average from 1 to about 50.
Claim 9
Dependent Claim
15 words
The reagent means of claim 6 wherein said carrier material is a protein or polypeptide.
Claim 10
Dependent Claim
22 words
The reagent means of claim 5 wherein the labeled reagent has a detectable property which is altered when bound with said antibody.
Claim 11
Dependent Claim
24 words
The reagent means of claim 5 in the form of a test kit wherein said antibody and labeled reagent are held in separate containers.
Claim 12
Dependent Claim
35 words
The reagent means of claim 8 in the form of a test device comprising a solid carrier member incorporated with said antibody to theophylline and said labeled form of theophylline or binding analog of theophylline.
Claim 13
Dependent Claim
27 words
The reagent means of claim 5 wherein said labeled reagent has the formula: ##STR22## wherein --CO)βGU is ##STR23## and n is an integer from 1 through 10.
Claim 14
Dependent Claim
38 words
The reagent means of claim 5 wherein said labeled reagent has the formula: ##STR24## wherein FAD is flavin adenine dinucleotide or a derivative thereof, Y is an amide group, and n is an integer from 1 through 10.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-08-25 | 1990-02-24 | 1990-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-08-25 | 1994-02-24 | 1994-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-08-25 | 1998-02-24 | 1998-08-26 | $7700.00 | Window Closed / Lapsed |