US4302438A
Expired / Lapsed
US
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Antigen, antiserum and immunoassay for theophylline
Assignee: Takeda GmbH
•
Filing: 1980-01-08
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Granted: 1981-11-24
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Expires: 2000-01-08
Abstract
Abstract By injecting a host animal with a member of a particular group of antigens [a theophylline-(7)-alkanecarboxylic acid covalently bonded to an immunogenic carrier (IGC) via the carboxyl group], antibodies, advantageously employed in determining small amounts of theophylline in biological liquids by immunoassay methods, are obtained.
Inventors
- 👤 Karl Zech
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 05:59
CPC Classifications
C; C07; C07D; C07D473/00; C07D473/02; C07D473/04; C07D473/06; C07D473/08; C07D311/00; C07D311/02; C07D311/04; C07D311/42; C07D311/44; C07D311/54; C07K; C07K16/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/94; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/975; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/815; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 41
Claim 1
Independent Claim
39 words
An antigen in the structure of which a haptene of the formula T--(CH.sub.2).sub.n --COOH wherein T is theophylline-(7) and n is an integer from 2 to 4, inclusive, is covalently bonded via the carbonyl group to an immunogenic carrier.
Claim 2
Dependent Claim
39 words
An antigen in the structure of which a haptene of the formula T--(CH.sub.2).sub.n --COOH wherein T is theophylline-(7) and n is an integer from 2 to 4, inclusive, is covalently bonded via the carbonyl group to an immunogenic carrier.
Claim 3
Dependent Claim
13 words
An antigen according to claim 1 wherein the immunogenic carrier is a protein.
Claim 4
Dependent Claim
34 words
An antigen according to claim 2 wherein the protein is a mammalian serum protein selected from the group consisting of human γ-globulin, human serum albumin, hare serum albumin, bovine γ-globulin and bovine serum albumin.
Claim 5
Dependent Claim
13 words
An antigen according to claim 1 wherein the immunogenic carrier is a polypeptide.
Claim 6
Dependent Claim
29 words
An antigen according to claim 4 wherein the polypeptide is a synthetic polypeptide selected from the group consisting of a polymer of lysine and a polymer of glutamic acid.
Claim 7
Dependent Claim
10 words
An antigen according to claim 1 wherein n is 3.
Claim 8
Dependent Claim
14 words
An antigen according to claim 1 wherein the immunogenic carrier is bovine serum albumin.
Claim 9
Dependent Claim
14 words
Antigen-stimulated animal-blood antiserum for which the antigen is an antigen according to claim 1.
Claim 10
Dependent Claim
17 words
A reagent for theophylline determination comprising an admixture of labelled theophylline and antiserum according to claim 8.
Claim 11
Dependent Claim
14 words
A theophylline/antibody complex wherein the antibody is that of antiserum according to claim 8.
Claim 12
Dependent Claim
12 words
A complex according to claim 10 wherein the theophylline is labelled theophylline.
Claim 13
Dependent Claim
14 words
A theophylline/antibody complex according to claim 11 wherein the labelled theophylline is radioactively labelled.
Claim 14
Dependent Claim
32 words
A complex according to claim 12 wherein the radioactively-labelled theophylline is that which is labelled with a radioisotope selected from the group consisting of tritium, carbon 14, iodine 125 and iodine 131.
Claim 15
Dependent Claim
18 words
A complex according to claim 12 wherein the radioactively-labelled theophylline is theophylline-(7)-butyric acid N-[2-(4-hydroxyphenyl)ethyl]amide iodinated with 125 iodine.
Claim 16
Dependent Claim
18 words
A complex according to claim 12 wherein the radioactively-labelled thoephylline is theophylline-(7)-butyric acid N-(2-imidazol-4-ylethyl)amide iodinated with 125 iodine.
Claim 17
Dependent Claim
22 words
A complex according to claim 12 wherein the radioactively-labelled theophylline is theophylline-(7)-butyryl-tyrosine, -tyrosine methylester or -tyrosine ethyl ester iodinated with 125 iodine.
Claim 18
Dependent Claim
14 words
A theophylline/antibody complex according to claim 11 wherein the labelled theophylline is enzyme labelled.
Claim 19
Dependent Claim
17 words
A complex according to claim 17 wherein the enzyme-labelled theophylline is that which is labelled with glucose-6-phosphate-dehydrogenase.
Claim 20
Dependent Claim
14 words
A theophylline/antibody complex according to claim 11 wherein the labelled theophylline is fluorescent labelled.
Claim 21
Dependent Claim
17 words
A complex according to claim 19 wherein the fluorescent-labelled theophylline is that which is labelled with 7-methoxy-4-methylcoumarin.
Claim 22
Dependent Claim
17 words
A complex according to claim 19 wherein the fluorescent-labelled theophylline is that which is labelled with fluorescein.
Claim 23
Dependent Claim
16 words
A theophylline/antibody complex according to claim 10 wherein the theophylline comprises both labelled and unlabelled form.
Claim 24
Dependent Claim
8 words
An antibody of antiserum according to claim 8.
Claim 25
Dependent Claim
31 words
In a process of producing antiserum useful for theophylline determination which comprises injecting a host animal with an antigen the improvement wherein the antigen is an antigen according to claim 1.
Claim 26
Dependent Claim
40 words
A theophylline determination method which comprises forming a theophylline/antibody complex by admixing a sample having an unknown theophylline content with a known amount of labelled theophylline and an antibody according to claim 23 and detecting the amount of labelled theophylline.
Claim 27
Dependent Claim
41 words
A method according to claim 25 which further comprises measuring the degree of binding of the labelled theophylline and comparing same against a standard curve obtained by adding known amounts of theophylline to a mixture of labelled theophylline and the antibody.
Claim 28
Dependent Claim
13 words
A method according to claim 26 wherein the labelled theophylline is radio-labelled theophylline.
Claim 29
Dependent Claim
13 words
A method according to claim 26 wherein the labelled theophylline is enzyme-labelled theophylline.
Claim 30
Dependent Claim
13 words
A method according to claim 26 wherein the labelled theophylline is fluorescence-labelled theophylline.
Claim 31
Dependent Claim
42 words
In an immunoassay reagent composition in which antiserum is in admixture with a labelled compound, the improvement wherein the antiserum is mammal-blood antiserum according to claim 8 and the labelled compound is one which forms a complex with antibodies of said antiserum.
Claim 32
Dependent Claim
26 words
An immunoassay reagent composition according to claim 30 for theophylline determination and in which the labelled compound is labelled theophylline or a labelled derivative of theophylline.
Claim 33
Dependent Claim
13 words
A reagent according to claim 31 wherein the labelled compound is radioactively labelled.
Claim 34
Dependent Claim
18 words
A reagent according to claim 32 wherein the radioactively-labelled compound is theophylline-(7)-butyric acid N-[2-(4-hydroxyphenyl)ethyl]amide iodinated with 125 iodine.
Claim 35
Dependent Claim
18 words
A reagent according to claim 32 wherein the radioactively-labelled compound is theophylline-(7)-butyric acid N-(2-imidazol-4-ylethyl)amide iodinated with 125 iodine.
Claim 36
Dependent Claim
22 words
A reagent according to claim 32 wherein the radioactively-labelled compound is theophylline-(7)-butyryl-tyrosin, -tyrosin methylester or -tyrosin ethyl ester iodinated with 125 iodine.
Claim 37
Dependent Claim
13 words
A reagent according to claim 31 wherein the labelled compound is enzyme labelled.
Claim 38
Dependent Claim
14 words
A reagent according to claim 36 wherein the enzyme-labelled compound is labelled with glucose-6-phosphate-dehydrogenase.
Claim 39
Dependent Claim
13 words
A reagent according to claim 31 wherein the labelled compound is fluorescent labelled.
Claim 40
Dependent Claim
14 words
A reagent according to claim 38 wherein the fluorescent-labelled compound is labelled with 7-methoxy-4-methylcoumarin.
Claim 41
Dependent Claim
14 words
A reagent according to claim 38 wherein the fluorescent-labelled compound is labelled with fluorescein.
References Cited (Backward Citations)
US4156081A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-23 | 1985-05-25 | 1985-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-23 | 1989-05-25 | 1989-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-23 | 1993-05-25 | 1993-11-24 | $7700.00 | Window Closed / Lapsed |