US4156081A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Theophylline antigens and antibodies
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1977-04-15
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Granted: 1979-05-22
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Expires: 1997-04-15
Abstract
Abstract Theophylline derivatives are provided for preparing reagents for use in competitive protein binding assays and for use as reagents in competitive protein binding assays. Theophylline is substituted at the 3 position and conjugated to antigens for production of antibodies which specifically recognize theophylline as distinct from structurally similar analogs such as caffeine. Enzyme conjugates are provided which find use for measuring the amount of theophylline in a sample suspected of containing theophylline. A method is provided employing the reagents for the determination of theophylline.
Inventors
- 👤 Prithipal Singh
- 👤 Mae W. Hu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 00:32
CPC Classifications
C; C07; C07D; C07D473/00; C07D473/02; C07D473/04; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/964; Y10S530/00; Y10S530/807
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
92 words
A compound of the formula ##STR5## wherein: R 1 is a aliphatic linking group having from 0 to 1 site of ethylenic unsaturation and of from 1 to 6 atoms other than hydrogen which are carbon, oxygen and not more than one nitrogen, wherein said oxygen is present as the carbonyl of amido or non-terminal ether and said nitrogen is present as the nitrogen of amido or tert-amino; and Z is aldehydo, carboxy, p-nitrophenyl or N-succinimidyloxy carboxyester, alkoxyimido, wherein the alkoxy group is of from 1 to 3 carbon atoms, or cyano.
Claim 2
Dependent Claim
92 words
A compound of the formula ##STR5## wherein: R 1 is a aliphatic linking group having from 0 to 1 site of ethylenic unsaturation and of from 1 to 6 atoms other than hydrogen which are carbon, oxygen and not more than one nitrogen, wherein said oxygen is present as the carbonyl of amido or non-terminal ether and said nitrogen is present as the nitrogen of amido or tert-amino; and Z is aldehydo, carboxy, p-nitrophenyl or N-succinimidyloxy carboxyester, alkoxyimido, wherein the alkoxy group is of from 1 to 3 carbon atoms, or cyano.
Claim 3
Dependent Claim
17 words
A compound according to claim 1, wherein R 1 is alkylene and Z is a carboxylic acid.
Claim 4
Dependent Claim
12 words
A compound according to claim 1, wherein Z is a carboxylic acid.
Claim 5
Dependent Claim
15 words
A compound according to claim 1, wherein R 1 is alkylene and Z is cyano.
Claim 6
Dependent Claim
6 words
A compound of the formula: ##STR6##
Claim 7
Dependent Claim
6 words
A compound of the formula: ##STR6##
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-05-21 | 1982-11-20 | 1983-05-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-05-21 | 1986-11-20 | 1987-05-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-05-21 | 1990-11-20 | 1991-05-22 | $7700.00 | Window Closed / Lapsed |