US4607008A
Expired / Lapsed
US
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Enzyme/immunofluorescent assay for anti-Epstein-Barr Virus antibodies
Assignee: CNA Holdings LLC
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Filing: 1984-03-05
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Granted: 1986-08-19
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Expires: 2004-03-05
Abstract
Abstract A method for the determination of anti-Epstein-Barr Virus antibodies in a test sample comprises contacting a substrate for the anti-Epstein-Barr Virus antibodies with sample; treating the contacted substrate with labeled antihuman Ig antibody selected from (a) a mixture comprising enzyme labeled antihuman Ig antibody and fluorescent labeled antihuman Ig antibody, (b) antihuman Ig antibodies labeled with an enzyme and a fluorescent label, (c) fluorescent labeled antihuman Ig antibody to which enzyme labeled antibody against the animal species from which the antibody used in the fluorescent labeled antibody was derived is subsequently added, and (d) enzyme labeled antihuman Ig antibody to which fluorescent labeled antibody against the animal species from which the antibody used in the enzyme labeled antibody was derived is subsequently added; determining the enzyme activity of the treated substrate; and determining the immunofluorescent patterns in substrates exhibiting enzyme activity. The method is useful for the rapid screening for anti-Epstein-Barr Virus antibodies for the diagnosis of Infectious Mononucleosis.
Inventors
- 👤 Stephen R. Coates
- 👤 Walter L. Binder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 10:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/581; G01N33/53; G01N33/569; G01N33/56983; G01N33/582; G01N2474/00; G01N2474/20; Y; Y10; Y10S; Y10S435/00; Y10S435/968
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
157 words
A method for the determination of anti-Epstein-Barr Virus antibody in a test sample, comprising: providing a substrate for the anti-Epstein-Barr Virus antibody; contacting with a test sample; treating said contacted substrate with labeled antihuman immunoglobulin antibody, said labeled antihuman immunoglobulin antibody selected from the group consisting of: (a) a mixture comprising enzyme labeled antihuman immunoglobulin antibody and fluorescent labeled antihuman immunoglobulin antibody; (b) antihuman immunoglobulin antibody labeled with an enzyme and a fluorescent label; (c) fluorescent labeled antihuman immunoglobulin antibody to which enzyme labeled antibody against the animal species from which the antibody used in the fluorescent labeled antibody was derived is subsequently added; and (d) enzyme labeled antihuman immunoglobulin antibody to which fluorescent labeled antibody against the animal species from which the antibody used in the enzyme labeled antibody was derived is subsequently added; analyzing the treated substrate to determine whether it has enzyme activity; and determining the immunofluorescent pattern in a substrate exhibiting enzyme activity.
Claim 2
Independent Claim
157 words
A method for the determination of anti-Epstein-Barr Virus antibody in a test sample, comprising: providing a substrate for the anti-Epstein-Barr Virus antibody; contacting with a test sample; treating said contacted substrate with labeled antihuman immunoglobulin antibody, said labeled antihuman immunoglobulin antibody selected from the group consisting of: (a) a mixture comprising enzyme labeled antihuman immunoglobulin antibody and fluorescent labeled antihuman immunoglobulin antibody; (b) antihuman immunoglobulin antibody labeled with an enzyme and a fluorescent label; (c) fluorescent labeled antihuman immunoglobulin antibody to which enzyme labeled antibody against the animal species from which the antibody used in the fluorescent labeled antibody was derived is subsequently added; and (d) enzyme labeled antihuman immunoglobulin antibody to which fluorescent labeled antibody against the animal species from which the antibody used in the enzyme labeled antibody was derived is subsequently added; analyzing the treated substrate to determine whether it has enzyme activity; and determining the immunofluorescent pattern in a substrate exhibiting enzyme activity.
Claim 3
Dependent Claim
13 words
The method according to claim 1 wherein said labeled antihuman antibody comprises (a).
Claim 4
Dependent Claim
13 words
The method according to claim 1 wherein said labeled antihuman antibody comprises (b).
Claim 5
Dependent Claim
13 words
The method according to claim 1 wherein said labeled antihuman antibody comprises (c).
Claim 6
Dependent Claim
13 words
The method according to claim 1 wherein said labeled antihuman antibody comprises (d).
Claim 7
Dependent Claim
12 words
The method according to claim 2 wherein said enzyme is horseradish peroxidase.
Claim 8
Dependent Claim
12 words
The method according to claim 3 wherein said enzyme is horseradish peroxidase.
Claim 9
Dependent Claim
12 words
The method according to claim 4 wherein said enzyme is horseradish peroxidase.
Claim 10
Dependent Claim
12 words
The method according to claim 2 wherein said fluorescent label is fluorescein.
Claim 11
Dependent Claim
12 words
The method acording to claim 3 wherein said fluorescent label is fluorescein.
Claim 12
Dependent Claim
12 words
The method according to claim 4 wherein said fluorescent label is fluorescein.
Claim 13
Dependent Claim
18 words
The method according to claim 2 wherein said enzyme is horseradish peroxidase and said fluorescent label is fluorescein.
Claim 14
Dependent Claim
18 words
The method according to claim 3 wherein said enyzme is horseradish peroxidase and said fluorescent label is fluorescein.
Claim 15
Dependent Claim
18 words
The method according to claim 4 wherein said enzyme is horseradish peroxidase and said fluorescent label is fluorescein.
Claim 16
Dependent Claim
13 words
The method according to claim 1 wherein said antihuman antibody is antihuman IgG.
Claim 17
Dependent Claim
16 words
The method according to claim 1 wherein said substrate comprises cell cultures infected with Epstein-Barr Virus.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-08-18 | 1990-02-17 | 1990-08-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-08-18 | 1994-02-17 | 1994-08-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-08-18 | 1998-02-17 | 1998-08-19 | $7700.00 | Window Closed / Lapsed |