US4487830A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme/immunofluorescent assay for autoantibodies
Assignee: Siemens Healthcare Diagnostics GmbH Germany
•
Filing: 1982-05-14
•
Granted: 1984-12-11
•
Expires: 2002-05-14
Abstract
Abstract A method for the determination of autoantibody in a test sample comprises contacting a substrate for the autoantibody with sample; treating the contacted substrate with labeled antihuman antibody selected from (1) a mixture comprising enzyme labeled antihuman antibody and fluorescent labeled antihuman antibody, and (2) antihuman antibody labeled with an enzyme and a fluorescent label; determining the enzyme activity of the treated substrate; and determining the immunofluorescent patterns in substrates exhibiting enzyme activity. The method is useful for the rapid screening and characterization of autoantibodies.
Inventors
- 👤 Stephen R. Coates
- 👤 Walter L. Binder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 20:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/531; G01N33/532; G01N33/564; G01N33/566; G01N33/567; G01N33/58; G01N33/581; G01N33/582; G01N2474/00; G01N2474/20; G01N2800/00; G01N2800/24
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
85 words
A method for the determination of autoantibody in a test sample, comprising: providing a substrate for said autoantibody; contacting said substrate with test sample; treating said contacted substrate with labeled antihuman antibody, said labeled antihuman antibody selected from the group consisting of: (1) a mixture comprising enzyme labeled antihuman antibody and fluorescent labeled antihuman antibody; and (2) antihuman antibody labeled with an enzyme and a fluorescent label; determining the enzyme activity of the treated substrate; and determining the immunofluorescent pattern in substrates exhibiting enzyme activity.
Claim 2
Independent Claim
85 words
A method for the determination of autoantibody in a test sample, comprising: providing a substrate for said autoantibody; contacting said substrate with test sample; treating said contacted substrate with labeled antihuman antibody, said labeled antihuman antibody selected from the group consisting of: (1) a mixture comprising enzyme labeled antihuman antibody and fluorescent labeled antihuman antibody; and (2) antihuman antibody labeled with an enzyme and a fluorescent label; determining the enzyme activity of the treated substrate; and determining the immunofluorescent pattern in substrates exhibiting enzyme activity.
Claim 3
Dependent Claim
24 words
A method according to claim 1 wherein said labeled antihuman antibody is a mixture comprising enzyme labeled antihuman antibody and fluorescent labeled antihuman antibody.
Claim 4
Dependent Claim
22 words
A method according to claim 1 wherein said labeled antihuman antibody is antihuman antibody labeled with an enzyme and a fluorescent label.
Claim 5
Dependent Claim
14 words
A method according to claim 2 or 3 wherein said enzyme is horseradish peroxidase.
Claim 6
Dependent Claim
14 words
A method according to claim 2 or 3 wherein said fluorescent label is fluorescein.
Claim 7
Dependent Claim
20 words
A method according to claim 2 or 3 wherein said enzyme is horseradish peroxidase and said fluorescent label is fluorescein.
Claim 8
Dependent Claim
12 words
A method according to claim 6 wherein said autoantibody comprises antinuclear antibodies.
Claim 9
Dependent Claim
13 words
A method according to claim 7 wherein said antihuman antibody is antihuman IgG.
Claim 10
Dependent Claim
12 words
A method according to claim 8 wherein said substrate comprises Hep-2 cells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-11 | 1988-06-11 | 1988-12-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-11 | 1992-06-11 | 1992-12-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-11 | 1996-06-11 | 1996-12-11 | $7700.00 | Window Closed / Lapsed |