Patent US4607007A successfully refreshed from Google Patents.
US4607007A
Expired / Lapsed
US
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Differentiation of natural killer cell subpopulations of cells
Assignee: Becton Dickinson and Co
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Filing: 1983-04-07
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Granted: 1986-08-19
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Expires: 2003-04-07
Abstract
Abstract A method for distinguishing multiple subpopulations of a cell sample whereby human natural killer cells subpopulations can be monitored. The method utilizes two monoclonal antibodies identified as anti-Leu-7 and anti-Leu-11.
Inventors
- 👤 Lewis Lanier
- 👤 Noel L. Warner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 05:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56966; G01N33/56972; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S435/973; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
124 words
A method for monitoring natural killer cell subpopulations in human blood comprising (a) providing a sample of cells containing a population of natural killer cells; (b) labelling selected cells of said sample with anti-Leu-11 monoclonal antibody coupled with a first fluorochrome label; (c) labelling selected cells of said sample with anti-Leu-7 monoclonal antibody coupled with a second fluorochrome label, said first fluorochrome label and said second fluorochrome label having a detectable emission difference; (d) providing excitation energy to excite said first fluorochrome label and said second fluorochrome label; (e) detecting the fluorescence emitted by the excited fluorochromes; and (f) distinguishing subpopulations of cells relative to the detected fluorescence characteristics thereof, whereby the relative proportion of natural killer cells in said subpopulations can be monitored.
Claim 2
Independent Claim
124 words
A method for monitoring natural killer cell subpopulations in human blood comprising (a) providing a sample of cells containing a population of natural killer cells; (b) labelling selected cells of said sample with anti-Leu-11 monoclonal antibody coupled with a first fluorochrome label; (c) labelling selected cells of said sample with anti-Leu-7 monoclonal antibody coupled with a second fluorochrome label, said first fluorochrome label and said second fluorochrome label having a detectable emission difference; (d) providing excitation energy to excite said first fluorochrome label and said second fluorochrome label; (e) detecting the fluorescence emitted by the excited fluorochromes; and (f) distinguishing subpopulations of cells relative to the detected fluorescence characteristics thereof, whereby the relative proportion of natural killer cells in said subpopulations can be monitored.
Claim 3
Dependent Claim
17 words
A method in accordance with claim 1 wherein said sample of cells is a peripheral blood sample.
Claim 4
Dependent Claim
38 words
A method in accordance with claim 2 wherein said blood sample is treated to provide a lymphocyte fraction comprising substantially all of the mononuclear cells including natural killer cells prior to the step of labelling said selected cells.
Claim 5
Dependent Claim
41 words
A method in accordance with claim 3 wherein said first fluorochrome label is selected from the group consisting of fluoroscein, rhodamine and a phycobiliprotein and said second fluorochrome label is selected from the group consisting of Texas Red and a phycobiliprotein.
Claim 6
Dependent Claim
20 words
A method in accordance with claim 3 wherein said first fluorochrome is fluorescein and said second fluorochrome is Texas Red.
Claim 7
Dependent Claim
19 words
A method in accordance with claim 3 wherein said first fluorochrome is fluorescein and said second fluorochrome is phycoerythrin.
Claim 8
Dependent Claim
40 words
A method in accordance with claim 1 wherein said labelled cells are passed, substantially one at a time, through an area of focused optical stimulation to provide excitation energy to excite said first fluorochrome label and said second fluorochrome label.
Claim 9
Dependent Claim
40 words
A method in accordance with claim 1 wherein said first fluorochrome label is selected from the group consisting of fluorescein, rhodamine and a phycobiliprotein and said second fluorochrome label is selected from the group consisting Texas Red and a phycobiliprotein.
Claim 10
Dependent Claim
20 words
A method in accordance with claim 1 wherein said first fluorochrome is fluorescein and said second fluorochrome is Texas Red.
Claim 11
Dependent Claim
19 words
A method in accordance with claim 1 wherein said first fluorochrome is fluorescein and said second fluorochrome is phycoerythrin.
Claim 12
Dependent Claim
19 words
A method in accordance with claim 1 wherein said fluorescence detection is accomplished by means of a fluorescence microscope.
Claim 13
Dependent Claim
13 words
A method in accordance with claim 1 wherein said fluorescence activated cell sorter.
References Cited (Backward Citations)
US4284412A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1989-08-18 | 1990-02-17 | 1990-08-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-08-18 | 1994-02-17 | 1994-08-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-08-18 | 1998-02-17 | 1998-08-19 | $7700.00 | Window Closed / Lapsed |