Patent US4604364A successfully refreshed from Google Patents.
US4604364A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Bioluminescent tracer composition and method of use in immunoassays
Assignee: Individual
•
Filing: 1983-04-21
•
Granted: 1986-08-05
•
Expires: 2003-04-21
Abstract
Abstract A tracer composition is described whereby a nonradioactive photon emitter is coupled to a ligand, antigen or antibody for use in various immunoassay methods of analysis. The photon emitters employed are bioluminescent proteins such as firefly or bacterial luciferase as well as other luciferases from various species. To synthesize the new tracer, these photon emitters are coupled to antigens or antibodies using coupling agents such as glutaraldehyde, CNBr, carbodiimide and others. The coupling method may also include intermediate materials such as polysaccharides, polypeptides, polyacrylamides and others coupled between the photon emitter and the antigen or antibody. Methods are described for using this tracer in both competitive and noncompetitive immunoassays to detect proteins, hormones, drugs, viruses and the like. Detection is accomplished by activating the tracer in a bioluminescent reaction and measuring the emitted light by photometric instruments or by photographic film. This new tracer composition has the advantages of nonradioactivity, high sensitivity and less endogeneous background activity.
Inventors
- 👤 Kenneth M. Kosak
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 18:05
CPC Classifications
G; G01; G01T; G01T1/00; G01T1/16; G01T1/20; G01N; G01N21/00; G01N21/75; G01N21/76; G01N21/763; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S436/00; Y10S436/80; Y10T; Y10T436/00; Y10T436/11; Y10T436/115831; Y10T436/13
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
46 words
A tracer composition comprising a material to be labelled selected from the group consisting of ligands and ligators, covalently coupled to luciferase through functional groups that are capable of coupling with glutaraldehyde, such functional groups being selected from amine, amide, phenolic hydroxyl, carbonyl and sulfhydryl groups.
Claim 2
Dependent Claim
46 words
A tracer composition comprising a material to be labelled selected from the group consisting of ligands and ligators, covalently coupled to luciferase through functional groups that are capable of coupling with glutaraldehyde, such functional groups being selected from amine, amide, phenolic hydroxyl, carbonyl and sulfhydryl groups.
Claim 3
Dependent Claim
11 words
The composition of claim 1, wherein said ligator is an antibody.
Claim 4
Dependent Claim
11 words
The composition of claim 1, wherein said ligand is an antigen.
Claim 5
Dependent Claim
36 words
The composition of claim 1, wherein said tracer composition includes an intermediate soluble support material such as proteins, polypeptides, polysaccharides, polyacrylamides and polyaldehydes through which said material to be labelled and said luciferase are covalently coupled.
Claim 6
Dependent Claim
11 words
The composition of claim 1, wherein said ligator is protein A.
Claim 7
Dependent Claim
30 words
The composition of claim 1, wherein said luciferase is selected from the group consisting of bacterial luciferase, firefly luciferase, coelenterate luciferase, crustacean luciferase, annelid luciferase, molluscan luciferase and dinoflagellate luciferase.
Claim 8
Dependent Claim
48 words
The composition of claim 1, wherein said ligator is primarily protein material selected from the group consisting of proteins, enzymes, intrinsic factor, globulins, biological receptors, ribosomes, cytochromes and lectins and wherein said material, or a derivative thereof, has ligand binding properties and the necessary functional groups for coupling.
Claim 9
Dependent Claim
41 words
The composition of claim 1, wherein said ligator is primarily non-protein material selected from the group consisting of steroids, RNA, and DNA and wherein said material, or a derivative thereof, has ligand binding properties and the necessary functional groups for coupling.
Claim 10
Dependent Claim
46 words
The composition of claim 1, wherein said ligand is primarily protein material selected from the group consisting of proteins, complement, hormones, interferons, viruses, microorganisms and blood cells and wherein said material, or a derivative thereof, has ligator binding properties and the necessary functional groups for coupling.
Claim 11
Dependent Claim
47 words
The composition of claim 1, wherein said ligand is primarily non-protein material selected from the group consisting of haptens, coenzymes, carbohydrates, lipids, antibiotics, drugs, pesticides and nucleic acids and wherein said material, or a derivative thereof, has ligator binding properties and the necessary functional groups for coupling.
Claim 12
Dependent Claim
59 words
The composition of claim 1, wherein said ligand is a vitamin of the B group, such as pyridoxines, or of the D group, such as calciferols, or vitamin H, such as biotin, or of the K group such as naphthoquinones and wherein said vitamin or a derivative thereof, has ligator binding properties and the necessary functional groups for coupling.
Claim 13
Dependent Claim
20 words
A tracer composition comprising an antibody covalently coupled to luciferase through functional groups that are capable of coupling with glutaraldehyde.
Claim 14
Dependent Claim
20 words
A tracer composition comprising an antibody covalently coupled to luciferase through functional groups that are capable of coupling with glutaraldehyde.
Claim 15
Dependent Claim
38 words
A tracer composition comprising a material to be labelled selected from the group consisting of ligands and ligators, covalently coupled to a bioluminescent photon emitter selected from the group consisting of photoproteins, aequorin, mnemiopsin, berovin, scintillons and lumisomes.
Claim 16
Dependent Claim
38 words
A tracer composition comprising a material to be labelled selected from the group consisting of ligands and ligators, covalently coupled to a bioluminescent photon emitter selected from the group consisting of photoproteins, aequorin, mnemiopsin, berovin, scintillons and lumisomes.
Claim 17
Dependent Claim
38 words
The composition of claim 13, wherein said tracer composition includes an intermediate soluble support material such as proteins, polypeptides, polysaccharides, polyacrylamides and polyaldehydes through which said material to be labelled and said bioluminescent photon emitter are covalently coupled.
Claim 18
Dependent Claim
11 words
The composition of claim 13 wherein said ligator is an antibody.
Claim 19
Dependent Claim
11 words
The composition of claim 13 wherein said ligand is an antigen.
Claim 20
Dependent Claim
130 words
In a nonradioactive immunoassay method for determining the presence of ligands and ligators in a competitive assay comprising (1) bringing together under conditions conducive to binding; (a) said ligand to be measured, with; (b) a ligator which has selective affinity for (a) and where the concentration of (b) is not sufficient to bind all of (a), with; (c) a labelled ligand which has selective affinity for (b) and competes with (a) for binding to (b); (2) and selectively determining the amount of unbound (c) and of (c) bound to (b); the improvement wherein said labelled ligand is a bioluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to luciferase and wherein step 2 includes the use of a bioluminescent reaction for detection.
Claim 21
Dependent Claim
130 words
In a nonradioactive immunoassay method for determining the presence of ligands and ligators in a competitive assay comprising (1) bringing together under conditions conducive to binding; (a) said ligand to be measured, with; (b) a ligator which has selective affinity for (a) and where the concentration of (b) is not sufficient to bind all of (a), with; (c) a labelled ligand which has selective affinity for (b) and competes with (a) for binding to (b); (2) and selectively determining the amount of unbound (c) and of (c) bound to (b); the improvement wherein said labelled ligand is a bioluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to luciferase and wherein step 2 includes the use of a bioluminescent reaction for detection.
Claim 22
Dependent Claim
127 words
In a nonradioactive immunoassay method for determining the presence of ligands and ligators in a noncompetitive "sandwich" assay comprising (1) bringing together under conditions conducive to binding; (a) said ligand to be measured, with; (b) an insolubilized ligator which has selective affinity for (a) and where the concentration of (b) is sufficient to bind all of (a) noncompetively, with; (c) a labelled ligator which has selective affinity for (a) but not (b) and; (2) selectively determining the amount of (c) bound to (a) and of unbound (c); the improvement wherein said labelled ligator is a bioluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to luciferase and wherein step 2 includes the use of a bioluminescent reaction for detection.
Claim 23
Dependent Claim
127 words
In a nonradioactive immunoassay method for determining the presence of ligands and ligators in a noncompetitive "sandwich" assay comprising (1) bringing together under conditions conducive to binding; (a) said ligand to be measured, with; (b) an insolubilized ligator which has selective affinity for (a) and where the concentration of (b) is sufficient to bind all of (a) noncompetively, with; (c) a labelled ligator which has selective affinity for (a) but not (b) and; (2) selectively determining the amount of (c) bound to (a) and of unbound (c); the improvement wherein said labelled ligator is a bioluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to luciferase and wherein step 2 includes the use of a bioluminescent reaction for detection.
Claim 24
Dependent Claim
26 words
The method of claim 17 or 18, wherein said selective determination of step 2 includes exposing photographic film to the light emitted from said bioluminescent reaction.
Claim 25
Dependent Claim
145 words
In a nonradioactive assay method for determining the presence of ligands and ligators in a competitive binding assay comprising, (1) bringing together under conditions conducive to binding; (a) said ligand to be measured with; (b) a ligator which has selective affinity for (a) and where the concentration of (b) is not sufficient to bind all of (a), with; (c) a labelled ligand which has selective affinity for (b) and competes for binding to (b); (2) and selectively determining the amount of unbound (c) and of (c) bound to (b); the improvement wherein said labelled ligand is a bioluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to a bioluminescent photon emitter selected from the group consisting of photoproteins, aequorin, mnemiopsin, berovin, scintillons and lumisomes and wherein step 2 includes the use of a bioluminescent reaction for detection.
Claim 26
Dependent Claim
145 words
In a nonradioactive assay method for determining the presence of ligands and ligators in a competitive binding assay comprising, (1) bringing together under conditions conducive to binding; (a) said ligand to be measured with; (b) a ligator which has selective affinity for (a) and where the concentration of (b) is not sufficient to bind all of (a), with; (c) a labelled ligand which has selective affinity for (b) and competes for binding to (b); (2) and selectively determining the amount of unbound (c) and of (c) bound to (b); the improvement wherein said labelled ligand is a bioluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to a bioluminescent photon emitter selected from the group consisting of photoproteins, aequorin, mnemiopsin, berovin, scintillons and lumisomes and wherein step 2 includes the use of a bioluminescent reaction for detection.
Claim 27
Dependent Claim
23 words
The method of claim 20, wherein selective determination of step 2 includes exposing photographic film to the light emitted from said bioluminescent reaction.
Claim 28
Dependent Claim
143 words
In a nonradioactive assay method for determining the presence of ligands and ligators in a noncompetitive "sandwich" assay comprising, (1) bringing together under conditions conductive to binding; (a) said ligand to be measured with; (b) an insolubilized ligator which has selective affinity for (a) and where the concentration of (b) is sufficient to bind all of (a) noncompetitively, with; (c) a labelled ligator which has selective affinity for (a) but not (b) and (2) selectively determining the amount of (c) bound to (a) and of unbound (c); the improvement wherein said labelled ligator is a bioluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to a bioluminescent photon emitter selected from the group consisting of photoproteins, aequorin, mnemiopsin, berovin, scintillons and lumisomes and wherein step 2 includes the use of a bioluminescent reaction for detection.
Claim 29
Dependent Claim
143 words
In a nonradioactive assay method for determining the presence of ligands and ligators in a noncompetitive "sandwich" assay comprising, (1) bringing together under conditions conductive to binding; (a) said ligand to be measured with; (b) an insolubilized ligator which has selective affinity for (a) and where the concentration of (b) is sufficient to bind all of (a) noncompetitively, with; (c) a labelled ligator which has selective affinity for (a) but not (b) and (2) selectively determining the amount of (c) bound to (a) and of unbound (c); the improvement wherein said labelled ligator is a bioluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to a bioluminescent photon emitter selected from the group consisting of photoproteins, aequorin, mnemiopsin, berovin, scintillons and lumisomes and wherein step 2 includes the use of a bioluminescent reaction for detection.
Claim 30
Dependent Claim
23 words
The method of claim 22, wherein selective determination of step 2 includes exposing photographic film to the light emitted from said bioluminescent reaction.
Claim 31
Dependent Claim
146 words
In a nonradioactive assay method for determining the presence of ligands and ligators in a noncompetitive "sandwich" assay comprising, (1) bringing together under conditions conducive to binding; (a) said ligand to be measured with; (b) an insolubilized ligator which has selective affinity for (a) and where the concentration of (b) is sufficient to bind all of (a) noncompetitively, with; (c) a labelled ligator which has selective affinity for (a) but not (b) and (2) selectively determining the amount of (c) bound to (a) and of unbound (c); the improvement wherein said labelled ligator is a chemiluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to a chemiluminescent material selected from the group consisting of luminol, lucigenin, cyclic hydrazides, peroxyoxalates, dioxetanes, diazaquinone and their respective derivatives and wherein step 2 includes the use of a chemiluminescent reaction for detection.
Claim 32
Dependent Claim
146 words
In a nonradioactive assay method for determining the presence of ligands and ligators in a noncompetitive "sandwich" assay comprising, (1) bringing together under conditions conducive to binding; (a) said ligand to be measured with; (b) an insolubilized ligator which has selective affinity for (a) and where the concentration of (b) is sufficient to bind all of (a) noncompetitively, with; (c) a labelled ligator which has selective affinity for (a) but not (b) and (2) selectively determining the amount of (c) bound to (a) and of unbound (c); the improvement wherein said labelled ligator is a chemiluminescent tracer composition comprising a material selected from the group consisting of ligands and ligators covalently coupled to a chemiluminescent material selected from the group consisting of luminol, lucigenin, cyclic hydrazides, peroxyoxalates, dioxetanes, diazaquinone and their respective derivatives and wherein step 2 includes the use of a chemiluminescent reaction for detection.
Claim 33
Dependent Claim
23 words
The method of claim 24, wherein selective determination of step 2 includes exposing photographic film to the light emitted from said chemiluminescent reaction.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-08-04 | 1990-02-03 | 1990-08-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-08-04 | 1994-02-03 | 1994-08-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-08-04 | 1998-02-03 | 1998-08-05 | $7700.00 | Window Closed / Lapsed |