US4604182A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Perfluorosulfonic acid polymer-coated indicator electrodes
Assignee: Dade Behring Inc
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Filing: 1985-01-29
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Granted: 1986-08-05
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Expires: 2005-01-29
Abstract
Abstract A method is described for the amperometric determination of the concentration in a biological sample of a constituent which is a substrate for an oxidase enzyme by (1) catalyzing the substrate with the enzyme, thereby consuming O 2 and producing H 2 O 2 and (2) measuring the O 2 consumption or H 2 O 2 production by means of an inert metal indicator electrode intimately coated with a thin film of a perfluorosulfonic acid polymer. A reaction chamber is described which provides means for contacting the sample with the enzyme and means for making amperometric measurements utilizing inert metal indicator electrodes coated with a thin film of a perfluorosulfonic acid polymer.
Inventors
- 👤 James L. Seago
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 16:04
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12M; C12M21/00; C12M21/18; C12M41/00; C12M41/30; C12M41/32
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
150 words
A flow-through enzyme reactor for the amperometric determination of a constituent of interest contained in a liquid sample, comprising: a first and a second end member, each having an internal chamber communicable with a source and a sink, respectively, of said liquid sample, said first and second chambers each having disposed therein a noble metal wire having coated thereon a thin film of a perfluorosulfonic acid polymer, said wires being externally connectable to electronic circuitry, said end members being made from an insulating material; and a central member having an internal chamber adapted to contain an immobilized oxidase enzyme, said chamber in communication with said internal chambers of said end members, said central chamber being separated from each of said end chambers by a foraminous septum, said septum being capable of containing the immobilized enzyme within the central chamber, said central chamber being made from an inert, electrically conductive material.
Claim 2
Dependent Claim
150 words
A flow-through enzyme reactor for the amperometric determination of a constituent of interest contained in a liquid sample, comprising: a first and a second end member, each having an internal chamber communicable with a source and a sink, respectively, of said liquid sample, said first and second chambers each having disposed therein a noble metal wire having coated thereon a thin film of a perfluorosulfonic acid polymer, said wires being externally connectable to electronic circuitry, said end members being made from an insulating material; and a central member having an internal chamber adapted to contain an immobilized oxidase enzyme, said chamber in communication with said internal chambers of said end members, said central chamber being separated from each of said end chambers by a foraminous septum, said septum being capable of containing the immobilized enzyme within the central chamber, said central chamber being made from an inert, electrically conductive material.
Claim 3
Dependent Claim
20 words
The reactor of claim 1 wherein the noble metal wire is selected from the group consisting of platinum and gold.
Claim 4
Dependent Claim
12 words
The reactor of claim 2 wherein the noble metal wire is platinum.
Claim 5
Dependent Claim
15 words
The reactor of claim 1 wherein the thin film is less than about 0.005 inch.
Claim 6
Dependent Claim
13 words
The reactor of claim 1 wherein the thin film is about 0.001 inch.
Claim 7
Dependent Claim
19 words
The reactor of claim 1 wherein the perfluorosulfonic acid polymer has an equivalent weight of at least about 900.
Claim 8
Dependent Claim
17 words
The reactor of claim 6 wherein the perfluorosulfonic acid polymer has an equivalent weight of about 1100.
Claim 9
Dependent Claim
32 words
The reactor of claim 1 wherein the central member is made from an inert conductive material selected from the group consisting of graphite, wax-filled graphite, polymer-filled graphite, pyrolitic graphite and glassy carbon.
Claim 10
Dependent Claim
13 words
The reactor of claim 8 wherein the inert conductive material is glassy carbon.
Claim 11
Dependent Claim
14 words
The reactor of claim 1 wherein the noble metal wire is about 38 gauge.
Claim 12
Dependent Claim
13 words
The reactor of claim 1 wherein the foraminous septum is a nylon mesh.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-08-04 | 1990-02-03 | 1990-08-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-08-04 | 1994-02-03 | 1994-08-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-08-04 | 1998-02-03 | 1998-08-05 | $7700.00 | Window Closed / Lapsed |